Development of a highly sensitive RNA virus detection method by artificial nucleoproteins
| Project/Area Number |
26640125
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical genome science
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 遺伝子診断 / 人工核蛋白質 / ゲノム目視検出 / 人工ヌクレオタンパク質 / 擬核蛋白質 |
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| Outline of Final Research Achievements |
As RNA viruses infect cells, the viral RNA and proteins are replicated within the cells to make its progeny viruses. During the infection process, the viral RNA makes a ribonucleoprotein complex with viral nucleoprotein to prevents it degradation by RNases in cells.
In this study, we synthesized a series of artificial peptide those were inspired from influenza viral RNA binding ribonucleoprotein domain sequence.Their artificial peptides were modified with gold-nanoparticle to detect influenza viral RNA.
As a result, we could succeeded to enhance the detection sensitivity of influenza virus RNA genome with gold-nanparticle conjugated artificial peptides.
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Report
(3 results)
Research Products
(3 results)
