| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Proteins function only when the polypeptide chain folds into its correct shape. Proteins from animals and plants need eukaryote-specific chaperonins for the correct folding, which lack in Escherichia coli. For future establishment of technologies for synthesizing eukaryotic proteins in E. coli, related technologies and some theory were studied. The first achievement was a plasmid construction method that could be useful for introduction of a dozen genes simultaneously in E. coli. The second was a method for optimization of gene sequences, with which researchers could change the sequences manually if necessary. The third achievement was a theory on relative expression levels of proteins in growing bacteria.
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