Constitutive understanding of the mechanism to generate DNA methylation pattern: a challenge toward synthetic epigenomics
| Project/Area Number |
26640131
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
System genome science
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| Research Institution | Kyushu University |
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Principal Investigator |
Ito Takashi 九州大学, 医学(系)研究科(研究院), 教授 (90201326)
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| Research Collaborator |
OKADA Satoshi 九州大学, 大学院医学研究院, 助教 (30734488)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | エピジェネティクス / ヒストン修飾 / DNAメチル化 / CRISPR/Cas9 / dCas9 / ヒストンアセチル化 / ヒストンメチル化 |
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| Outline of Final Research Achievements |
The pattern of epigenetic modifications including histone modifications and DNA methylation regulates the function of eukaryotic genome. Accordingly, if one can artificially generate a specific pattern, one may not only make the genome exert an intended function but also prove the mechanism underlying the formation of the pattern. To pursue the possibility of such synthetic epigenomics approaches, we exploited the CRISPR/Cas9 system to modify histone modification patterns at a specific locus in the yeast genome to confer an intended phenotype on the cells.
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Report
(3 results)
Research Products
(5 results)
