State analyses of a cell-free protein synthesis system using ribosome profiling
| Project/Area Number |
26640134
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
System genome science
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Shimizu Yoshihiro 国立研究開発法人理化学研究所, 生命システム研究センター, ユニットリーダー (90401231)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 無細胞タンパク質合成 / リボソーム / mRNA / 次世代シーケンサー |
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| Outline of Final Research Achievements |
We aimed to establish a technique to analyze how the internal state of the PURE system, a reconstituted cell-free protein synthesis system, changes according to the changes in mRNA sequence by using deep sequencing. By preparing an mRNA where 12 bases downstream of the start codon are randomized and translating it in the PURE system to obtain an initiation complex or elongation complex, only the mRNA sequence interacting with the ribosome is specifically extracted. It became possible to quantitatively evaluate the influence of mRNA sequence by performing deep sequence analysis.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Mammalian Reverse Genetics without Crossing Reveals Nr3a as a Short-Sleeper Gene.2016
Author(s)
Sunagawa GA, Sumiyama K, Ukai-Tadenuma M, Perrin D, Fujishima H, Ukai H, Nishimura O, Shi S, Ohno R, Narumi R, Shimizu Y, Tone D, Ode KL, Kuraku S, Ueda HR.
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Journal Title
Cell Rep.
Volume: 14
Issue: 3
Pages: 662-677
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis.2014
Author(s)
Etsuo A. Susaki, Kazuki Tainaka, Dimitri Perrin, Fumiaki Kishino, Takehiro Tawara, Tomonobu M. Watanabe, Chihiro Yokoyama, Hirotaka Onoe, Megumi Eguchi, Shun Yamaguchi, Takaya Abe, Hiroshi Kiyonari, Yoshihiro Shimizu, Atsushi Miyawaki, Hideo Yokota, Hiroki R. Ueda
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Journal Title
Cell
Volume: 157
Issue: 3
Pages: 726-739
DOI
Related Report
Peer Reviewed
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