| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
We aimed to establish a technique to analyze how the internal state of the PURE system, a reconstituted cell-free protein synthesis system, changes according to the changes in mRNA sequence by using deep sequencing. By preparing an mRNA where 12 bases downstream of the start codon are randomized and translating it in the PURE system to obtain an initiation complex or elongation complex, only the mRNA sequence interacting with the ribosome is specifically extracted. It became possible to quantitatively evaluate the influence of mRNA sequence by performing deep sequence analysis.
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