| Project/Area Number |
26640138
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservation of biological resources
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| Research Institution | Toyo University |
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Principal Investigator |
Miura Takeshi 東洋大学, 生命科学部, 准教授 (20416001)
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| Research Collaborator |
WATANABE Akihiro
NAKANO Shiori
KOBAYASHI Tohru
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | イオン強度 / 新規スクリーニング法 / マリアナ海溝底泥コアサンプル / スクリーニング法 / マリアナ海溝底泥サンプル / 有用酵素生産菌 |
|---|
| Outline of Final Research Achievements |
We obtained 30 cm of sediment cores from Mariana Trenth at a water depth of 10,900 m. A column (1×1 cm) of powder filter paper was washed with 100 mM sodium acetate buffer (pH4), Tris-HCl (pH7), CHES buffer (pH9). The core sediments suspension with sterilized buffer was applied to the column. The column was eluted with a continuous linear gradient of 0-6 M NaCl in sodium acetate buffer (pH4), Tris-HCl (pH7), CHES buffer (pH9). A dilution series of all fractions with sterilized beffer was spread onto a Marine Agar 2216 (pH7) plate, and they were incubated at 25℃ for 2 weeks. In the relationship between cultivable cell numbers and pH, the cultivable cell numbers were lower at pH4 in any agar plates than at pH7 and pH9, while the cultivable cell numbers decreased against the increase of NaCl concentration (Ionic strength). With this result, many new microorganisms by utilizing the difference in ionization degree showed the potential to be isolated.
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