| Project/Area Number |
26640139
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservation of biological resources
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| Research Institution | Fukuyama University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 毛根 / 哺乳動物 / 細胞培養 / 凍結保存 / 初期化 / エピソーマルベクター / 分化誘導 / ノアの箱舟 / ヒト / アカネズミ / マウス / ハムスター / 培養細胞 / マイクロアレイ解析 / ひげ / まゆげ / 添加物 / 培養基質 / 酵素処理 |
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| Outline of Final Research Achievements |
Using the eyebrow hair roots that could be easily removed by 7 adult volunteers themselves under informed consent, fibroblast-like normal cells were obtained with regardless of age or gender. By the same method, normal cells of other 5 species were cultured and stocked. To obtain normal cells as bio-resource from whisker or body hair of other mammals, we examined mouse, djungarian hamster and Japanese field mouse (Apodemus speciosus), syrian hamster and four toed hedgehog as model animals, then most of cultured cells were fibroblastic shape, but some morphological variation were observed. We also clarified the most suitable condition to obtain normal cells of these mammals. We cultured and freeze-stocked normal cells by a simple and minimally invasive way from the hair root of 6 species of mammals. These stocked normal cells will be deposited to the cell bank as valuable bio-resources.
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