| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The human-specific NBPF ncRNA, which is localized in nuclear bodies, is derived from the intronic regions of the human specific NBPF repeat cluster. By the experiment using a splicing inhibitor, we showed that NBPF ncRNAs are the excised introns which were created upon splicing of the precursor transcripts. Next generation RNA sequencing of the RNAs prepared from the cells in which NBPF ncRNA was specifically knocked down revealed that the expression of NOTCH2NL gene which is located adjacent to the NBPF cluster is activated by NBPF ncRNA. We also discovered that NBPF ncRNA exhibits semi-extractable feature in the process of the standard RNA preparation method, which is common to that of NEAT1 ncRNA localized in another nuclear body.
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