Identification of non-coding RNAs for heterochromatin formation on autosomes
Project/Area Number |
26650002
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Molecular biology
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Research Institution | Hokkaido University |
Principal Investigator |
Obuse Chikashi 北海道大学, 先端生命科学研究院, 教授 (00273855)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | クロマチン / エピジェネティクス / 非コードRNA / RNA / 非コード / ゲノム / 不活性X染色体 / XIST / エピゲノム |
Outline of Final Research Achievements |
The female inactive X chromosome, which has heterochromtic condensed structure throughout the whole chromosome mediated by noncoding RNA, XIST, is a good model for heterochromatin formation. In this study, we searched for non-coding RNAs that act like XIST on autosomes. As a result, proteins that are responsible for heterochromatinization with XIST, were enriched in the Prader-Willi syndrome region, suggesting that these proteins contribute to the condensation in this region. In addition, by the RNA FISH approach, several probes in this region showed positive signals, suggesting the possibility that non-coding RNAs like XIST transcribe from this region. These results will contribute for future study; how non-coding RNA is involved in functional higher order structure of chromatin on autosomes.
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Report
(4 results)
Research Products
(59 results)
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[Journal Article] BRCA1 Directs the Repair Pathway to Homologous Recombination by Promoting 53BP1 Dephosphorylation.2017
Author(s)
Isono M, Niimi A, Oike T, Hagiwara Y, Sato H, Sekine R, Yoshida Y, Isobe SY, Obuse C, Nishi R, Petricci E, Nakada S, Nakano T, Shibata A.
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Journal Title
Cell Reports
Volume: 18
Pages: 520-532
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Clinical, muscle pathological, and genetic features of Japanese facioscapulohumeral muscular dystrophy 2 (FSHD2) patients with SMCHD1 mutations.2016
Author(s)
Hamanaka K, Goto K, Arai M, Nagao K, Obuse C, Noguchi S, Hayashi YK, Mitsuhashi S, Nishino I.
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Journal Title
Neuromuscul Disorder
Volume: 26
Pages: 300-308
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Histone H4 lysine 20 acetylation is associated with gene repression in human cells.2016
Author(s)
Kaimori JY, Maehara K, Hayashi-Takanaka Y, Harada A, Fukuda M, Yamamoto S, Ichimaru N, Umehara T, Yokoyama S, Matsuda R, Ikura T, Nagao K, Obuse C, Nozaki N, Takahara S, Takao T, Ohkawa Y, Kimura H, Isaka Y.
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Journal Title
Scientific Reports
Volume: 6
Pages: 24318
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] HP1結合因子によるクロマチン制御2017
Author(s)
小布施力史
Organizer
ワークショップ「染色体研究の最前線」
Place of Presentation
大阪大学生命機能研究科(大阪府吹田市)
Year and Date
2017-01-16 – 2017-01-17
Related Report
Invited
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[Presentation] “Two Nup133-homologs Separately Function at the Cytoplasmic or Nuclear Side in Nuclear Pore Complex in Schizosaccharomyces Pombe“2015
Author(s)
Haruhiko Asakawa, Hui-Ju Yang, Tomoko Kojidani, Chizuru Ohtsuiki, Hiroko Osakada, Koji Nagao, Chikashi Obuse, Yasushi Hiraoka, Tokuko Haraguchi
Organizer
International Symposium on Chromatin Structure, Dynamics, and Function
Place of Presentation
淡路夢舞台(兵庫県・淡路市)
Year and Date
2015-08-23 – 2015-08-26
Related Report
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[Presentation] “Function of ORC-binding protein ORCBP1 in Human Cells“2015
Author(s)
YoshiNobu Ohkubo, Masahiro Yamaguchi, Takashi Seki, Ryu-suke Nozawa, Shinya Isobe, Syohei Ishimoto, Koji Nagao, Chikashi Obuse
Organizer
International Symposium on Chromatin Structure, Dynamics, and Function
Place of Presentation
淡路夢舞台(兵庫県・淡路市)
Year and Date
2015-08-23 – 2015-08-26
Related Report
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