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A cell-free system of Xenopus eggs that induces transcriptional reprogramming

Research Project

Project/Area Number 26650005
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Molecular biology
Research InstitutionNagoya University

Principal Investigator

IWABICHI MARI  名古屋大学, 理学(系)研究科(研究院), 講師 (40275350)

Co-Investigator(Renkei-kenkyūsha) OHSUMI Keita  名古屋大学, 理学研究科, 教授 (20221822)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords核アクチン / 卵無細胞系 / 核抽出液
Outline of Final Research Achievements

Understanding the regulatory mechanism of the cellular potency for differentiation is one of important goals in biological sciences. Based on a recent report by the Gurdon’s group that nuclear F-actin is involved in transcriptional reprogramming induced in the frog oocyte nucleus, we tried to prepare nucleoplasmic extract by the use of frog egg extracts that are able to reproduce intracellular actin dynamics. We have not been successful in its preparation because of technical difficulties but instead have found that in eggs and blastula cells, the early embryonic subtype of an actin-binding protein promotes actin polymerization in the nucleus.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2016 2015 2014

All Presentation (6 results) (of which Invited: 1 results)

  • [Presentation] 胞胚型核システムの分子基盤2016

    • Author(s)
      岩渕万里、小野田衣里、小田春佳、大隅圭太
    • Organizer
      第33回染色体ワークショップ・第14回核ダイナミクス研究会
    • Place of Presentation
      松島一の坊
    • Year and Date
      2016-01-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] 胞胚細胞のF-actinによるクロマチン-核膜結合の役割2016

    • Author(s)
      小田春佳、白井菜月、大隅圭太、岩渕万里
    • Organizer
      第33回染色体ワークショップ・第14回核ダイナミクス研究会
    • Place of Presentation
      松島一の坊
    • Year and Date
      2016-01-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] カエル胞胚細胞の核システム2015

    • Author(s)
      岩渕万里、小野田衣里、小田春佳、大隅圭太
    • Organizer
      日本動物学会第86回大会
    • Place of Presentation
      朱鷺メッセ
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] カエル初期胚における未分化細胞の核システム2014

    • Author(s)
      岩渕万里、小田春佳、白井菜月、浦菜緒子、大隅圭太
    • Organizer
      第32回染色体ワークショップ・第13回核ダイナミクス研究会
    • Place of Presentation
      安芸グランドホテル
    • Year and Date
      2014-12-16
    • Related Report
      2014 Research-status Report
  • [Presentation] アフリカツメガエル初期胚における核アクチンの解析2014

    • Author(s)
      白井菜月、小田春佳、浦菜緒子、大隅圭太、岩渕万里
    • Organizer
      第37回日本分子生物学会年会 ワークショップ
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25
    • Related Report
      2014 Research-status Report
  • [Presentation] 核アクチンフィラメントの動態を再現する新規ツメガエル卵無細胞系を用いたクロマチン-核膜結合制御機構の解析2014

    • Author(s)
      岩渕万里、浦菜緒子、小田春佳、白井菜月、大隅圭太
    • Organizer
      第87回日本生化学会 シンポジウム
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2014-10-18
    • Related Report
      2014 Research-status Report
    • Invited

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Published: 2014-04-04   Modified: 2017-05-10  

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