| Project/Area Number |
26650006
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
Kuraoka Isao 大阪大学, 基礎工学研究科, 准教授 (60335396)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
Iwai Shigenori 大阪大学, 大学院基礎工学研究科, 教授 (10168544)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | RNA / 損傷 / 核酸 / イノシン化RNA |
|---|
| Outline of Final Research Achievements |
Deamination of adenine occurs in DNA, RNA, and their precursors. The generated deaminated products are potentially mutagenic because of their structural similarity to natural bases. We found that human endonuclease V (i-RNase) recognizes and cleaves inosine-containing RNA.
In this study, to figure out the characters of the endonuclease activity, a series of RNA/DNA substrates was employed. Our data indicated that this enzyme is involved in RNA repair.
|
