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Functions of nuclear actin filament and its mechanism of the formation

Research Project

Project/Area Number 26650007
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Molecular biology
Research InstitutionOsaka University

Principal Investigator

Shinohara Akira  大阪大学, たんぱく質研究所, 教授 (00252578)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords減数分裂 / 染色体 / 核膜 / 細胞骨格 / アクチン繊維 / 核 / 核骨格
Outline of Final Research Achievements

During meiosis, telomeres are attached to the nuclear envelope (NE) and move to the vicinity of the centrosome. The movement of telomeres, thus chromosomes, is driven by forces, which is transmitted through NE from cytoplasmic cytoskeltons. Dynamic repositioning of telomeres is a unique feature of meiotic prophase I. Telomere clustering is thought to promote recognition, meiotic recombination, and stable pairing between the homologous chromosomes. However, the molecular basis of telomere attachment and movement as well as its regulation is largely unknown. Previous works suggest that the nuclear envelope (NE) plays a critical role in telomere attachment/movement during meiotic prophase I. We found that telomere clustering and movement depend on two cell cycle kinases, cyclin- dependent kinase (CDK) and Dbf4-dependent Cdc7 kinase (DDK). Particularly, the impairment of the two kinases during meiosis affects the localization and movement of a component of the SPB of S. cerevisiae, Mps3.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (21 results)

All 2016 2015 2014 Other

All Int'l Joint Research (3 results) Journal Article (8 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 8 results,  Acknowledgement Compliant: 7 results,  Open Access: 6 results) Presentation (8 results) (of which Int'l Joint Research: 3 results,  Invited: 6 results) Remarks (2 results)

  • [Int'l Joint Research] New York University(米国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Chung Ang University(韓国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2015 Annual Research Report
  • [Journal Article] The Paf1 Complex Shapes the Landscape of Double-strand Breaks along Meiotic Chromosomes in Saccharomyces cerevisiae2016

    • Author(s)
      Santosh, G. K., Patel, K.J., Colletti M.M., Sasanuma, H., Shinohara, M., Hochwagen, A., and A. Shinohara
    • Journal Title

      Genetics

      Volume: 202 Issue: 2 Pages: 497-512

    • DOI

      10.1534/genetics.115.177287

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Chromosome synapsis alleviates Mek1-dependent suppression of meiotic DNA repair.2016

    • Author(s)
      Subramanian, V.V., MacQueen, A. J., Vader, G., Shinohara, M., Borde, V., Shinohara, A. and A. Hochwagen
    • Journal Title

      Current Biology

      Volume: 14 Issue: 2 Pages: e1002369-e1002369

    • DOI

      10.1371/journal.pbio.1002369

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Rad61/Wpl1(Wapl), a cohesin regulator, controls chromosome compaction during meiosis.2016

    • Author(s)
      Challa, KK., Lee, MS., Shinohara, M., Kim, K.m and A. Shinohara.
    • Journal Title

      Nucleic Acids Research

      Volume: in press Issue: 7 Pages: 3190-3203

    • DOI

      10.1093/nar/gkw034

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] DNA damage response clamp contributes to chromosomal assembly of ZMM-SIC pro-crossover factors during meiosis2015

    • Author(s)
      Shinohara, M, Hayashihara, K., Grubb, J.T., Bishop, D.K., and A. Shinohara.
    • Journal Title

      J. Cell Science

      Volume: 128 Pages: 1494-1494

    • DOI

      10.1242/jcs.161554

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] ATM and SIRT6/SNF2H mediate transient H2AX stabilization when DSBs form by blocking HUWE1 to allow efficient γH2AX foci formation.2015

    • Author(s)
      Atsumi, Y., Minakawa, Y., Ono, M., Dobashi, S., Snohe, K., Shinohara, A., Takeda, S., Takagi, M., Takamatsu, N., Nakagama, H., Teraoka, H., and K.-I. Yoshioka
    • Journal Title

      Cell Reports

      Volume: 13 Issue: 12 Pages: 2728-2740

    • DOI

      10.1016/j.celrep.2015.11.054

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Dot1-dependent histone H3K79 methylation promotes the formation of meiotic double-strand breaks in the absence of histone H3K4 methylation in budding yeast.2014

    • Author(s)
      Bani Ismail, M., Shinohara M. and A. Shinohara.
    • Journal Title

      PLoS One

      Volume: 9 Issue: 5 Pages: e96648-e96648

    • DOI

      10.1371/journal.pone.0096648

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Canonical Non-homologous End Joining in Mitosis Induces Genome Instability and Is Suppressed by M-phase Specific Phosphorylation of XRCC4 via CDKs.2014

    • Author(s)
      Terasawa, M., Shinohara A., and M. Shinohara.
    • Journal Title

      PLoS Genetics

      Volume: 10 Issue: 8 Pages: e1004563-e1004563

    • DOI

      10.1371/journal.pgen.1004563

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Double-strand break repair-adox: restoration of suppressed double-strand break repair during mitosis induces genomic instability.2014

    • Author(s)
      Terasawa, M., Shinohara. A., and *M. Shinohara.
    • Journal Title

      Cancer Science

      Volume: 105 Issue: 12 Pages: 1519-1525

    • DOI

      10.1111/cas.12551

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Prophase pathway for cleavage-independent removal of cohesin from chromosome arms during late prophase-I in meiosis.2015

    • Author(s)
      Challa, K., Shinohara, M. A. Shinohara.
    • Organizer
      FAOBMB meeting
    • Place of Presentation
      Hyderabad, India
    • Year and Date
      2015-11-27
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Prophase pathway for cleavage-independent removal of cohesin from chromosome arms during late prophase-I in meiosis.2015

    • Author(s)
      Challa, K., Shinohara, M. A. Shinohara.
    • Organizer
      International RecA and chromosome dynamics meeting
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Prophase pathway for cleavage-independent removal of cohesin from chromosome arms during late prophase-I in meiosis.2015

    • Author(s)
      Challa, K., Shinohara, M. A. Shinohara.
    • Organizer
      EMBO workshop on meiosis
    • Place of Presentation
      Oxford, UK
    • Year and Date
      2015-09-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Prophase pathway for cleavage-independent removal of cohesin from chromosome arms during late prophase-I in meiosis.2015

    • Author(s)
      A. Shinohara
    • Organizer
      Meiosis meeting in Spain
    • Place of Presentation
      Salamanca, Spain
    • Year and Date
      2015-06-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Control mechanism of chromosome motion during meiosis2014

    • Author(s)
      Shinohara, A.
    • Organizer
      International conference on Chromosome Stability
    • Place of Presentation
      Bangalore, India
    • Year and Date
      2014-12-14 – 2014-12-17
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Control of Rad51 filament formation2014

    • Author(s)
      Shinohara, A.
    • Organizer
      Gordon Research Conference on Genome Instability
    • Place of Presentation
      Hong, Kong, China
    • Year and Date
      2014-07-06 – 2014-07-11
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Control mechanism of chromosome motion during meiosis2014

    • Author(s)
      Shinohara, A.
    • Organizer
      Gordon Research Conference on Meiosis
    • Place of Presentation
      New London, NH, USA
    • Year and Date
      2014-06-06 – 2014-06-11
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Control of Rad51 filament formation2014

    • Author(s)
      Shinohara, A.
    • Organizer
      Abacam meeting on Mechanism of recombination
    • Place of Presentation
      Alicante Spain
    • Year and Date
      2014-05-19 – 2014-05-23
    • Related Report
      2014 Research-status Report
    • Invited
  • [Remarks] 大阪大学蛋白質研究所ゲノム~染色体機能研究室ホームページ

    • URL

      http://www.protein.osaka-u.ac.jp/genome/Shinohara-HP-index.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 大阪大学 蛋白質研究所 ゲノムー染色体機能研究室HP

    • URL

      http://www.protein.osaka-u.ac.jp/genome/index.html

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2022-01-31  

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