Functions of nuclear actin filament and its mechanism of the formation

• Project/Area Number: 26650007
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Osaka University
• Principal Investigator: Shinohara Akira 大阪大学, たんぱく質研究所, 教授 (00252578)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: 減数分裂 / 染色体 / 核膜 / 細胞骨格 / アクチン繊維 / 核 / 核骨格

Outline of Final Research Achievements

During meiosis, telomeres are attached to the nuclear envelope (NE) and move to the vicinity of the centrosome. The movement of telomeres, thus chromosomes, is driven by forces, which is transmitted through NE from cytoplasmic cytoskeltons. Dynamic repositioning of telomeres is a unique feature of meiotic prophase I. Telomere clustering is thought to promote recognition, meiotic recombination, and stable pairing between the homologous chromosomes. However, the molecular basis of telomere attachment and movement as well as its regulation is largely unknown. Previous works suggest that the nuclear envelope (NE) plays a critical role in telomere attachment/movement during meiotic prophase I. We found that telomere clustering and movement depend on two cell cycle kinases, cyclin- dependent kinase (CDK) and Dbf4-dependent Cdc7 kinase (DDK). Particularly, the impairment of the two kinases during meiosis affects the localization and movement of a component of the SPB of S. cerevisiae, Mps3.

Research Products

• [Int'l Joint Research] New York University(米国)
• [Int'l Joint Research] Chung Ang University(韓国)
• [Int'l Joint Research]
• [Journal Article] The Paf1 Complex Shapes the Landscape of Double-strand Breaks along Meiotic Chromosomes in Saccharomyces cerevisiae (2016)
  • Author(s): Santosh, G. K., Patel, K.J., Colletti M.M., Sasanuma, H., Shinohara, M., Hochwagen, A., and A. Shinohara
  • Journal Title: Genetics Volume: 202 Issue: 2 Pages: 497-512
  • DOI: 10.1534/genetics.115.177287
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Chromosome synapsis alleviates Mek1-dependent suppression of meiotic DNA repair. (2016)
  • Author(s): Subramanian, V.V., MacQueen, A. J., Vader, G., Shinohara, M., Borde, V., Shinohara, A. and A. Hochwagen
  • Journal Title: Current Biology Volume: 14 Issue: 2 Pages: e1002369-e1002369
  • DOI: 10.1371/journal.pbio.1002369
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Rad61/Wpl1(Wapl), a cohesin regulator, controls chromosome compaction during meiosis. (2016)
  • Author(s): Challa, KK., Lee, MS., Shinohara, M., Kim, K.m and A. Shinohara.
  • Journal Title: Nucleic Acids Research Volume: in press Issue: 7 Pages: 3190-3203
  • DOI: 10.1093/nar/gkw034
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] DNA damage response clamp contributes to chromosomal assembly of ZMM-SIC pro-crossover factors during meiosis (2015)
  • Author(s): Shinohara, M, Hayashihara, K., Grubb, J.T., Bishop, D.K., and A. Shinohara.
  • Journal Title: J. Cell Science Volume: 128 Pages: 1494-1494
  • DOI: 10.1242/jcs.161554
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] ATM and SIRT6/SNF2H mediate transient H2AX stabilization when DSBs form by blocking HUWE1 to allow efficient γH2AX foci formation. (2015)
  • Author(s): Atsumi, Y., Minakawa, Y., Ono, M., Dobashi, S., Snohe, K., Shinohara, A., Takeda, S., Takagi, M., Takamatsu, N., Nakagama, H., Teraoka, H., and K.-I. Yoshioka
  • Journal Title: Cell Reports Volume: 13 Issue: 12 Pages: 2728-2740
  • DOI: 10.1016/j.celrep.2015.11.054
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dot1-dependent histone H3K79 methylation promotes the formation of meiotic double-strand breaks in the absence of histone H3K4 methylation in budding yeast. (2014)
  • Author(s): Bani Ismail, M., Shinohara M. and A. Shinohara.
  • Journal Title: PLoS One Volume: 9 Issue: 5 Pages: e96648-e96648
  • DOI: 10.1371/journal.pone.0096648
  • Peer Reviewed / Open Access
• [Journal Article] Canonical Non-homologous End Joining in Mitosis Induces Genome Instability and Is Suppressed by M-phase Specific Phosphorylation of XRCC4 via CDKs. (2014)
  • Author(s): Terasawa, M., Shinohara A., and M. Shinohara.
  • Journal Title: PLoS Genetics Volume: 10 Issue: 8 Pages: e1004563-e1004563
  • DOI: 10.1371/journal.pgen.1004563
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Double-strand break repair-adox: restoration of suppressed double-strand break repair during mitosis induces genomic instability. (2014)
  • Author(s): Terasawa, M., Shinohara. A., and *M. Shinohara.
  • Journal Title: Cancer Science Volume: 105 Issue: 12 Pages: 1519-1525
  • DOI: 10.1111/cas.12551
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Prophase pathway for cleavage-independent removal of cohesin from chromosome arms during late prophase-I in meiosis. (2015)
  • Author(s): Challa, K., Shinohara, M. A. Shinohara.
  • Organizer: FAOBMB meeting
  • Place of Presentation: Hyderabad, India
  • Year and Date: 2015-11-27
  • Invited
• [Presentation] Prophase pathway for cleavage-independent removal of cohesin from chromosome arms during late prophase-I in meiosis. (2015)
  • Author(s): Challa, K., Shinohara, M. A. Shinohara.
  • Organizer: International RecA and chromosome dynamics meeting
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2015-09-16
  • Int'l Joint Research / Invited
• [Presentation] Prophase pathway for cleavage-independent removal of cohesin from chromosome arms during late prophase-I in meiosis. (2015)
  • Author(s): Challa, K., Shinohara, M. A. Shinohara.
  • Organizer: EMBO workshop on meiosis
  • Place of Presentation: Oxford, UK
  • Year and Date: 2015-09-01
  • Int'l Joint Research
• [Presentation] Prophase pathway for cleavage-independent removal of cohesin from chromosome arms during late prophase-I in meiosis. (2015)
  • Author(s): A. Shinohara
  • Organizer: Meiosis meeting in Spain
  • Place of Presentation: Salamanca, Spain
  • Year and Date: 2015-06-04
  • Int'l Joint Research
• [Presentation] Control mechanism of chromosome motion during meiosis (2014)
  • Author(s): Shinohara, A.
  • Organizer: International conference on Chromosome Stability
  • Place of Presentation: Bangalore, India
  • Year and Date: 2014-12-14 – 2014-12-17
  • Invited
• [Presentation] Control of Rad51 filament formation (2014)
  • Author(s): Shinohara, A.
  • Organizer: Gordon Research Conference on Genome Instability
  • Place of Presentation: Hong, Kong, China
  • Year and Date: 2014-07-06 – 2014-07-11
  • Invited
• [Presentation] Control mechanism of chromosome motion during meiosis (2014)
  • Author(s): Shinohara, A.
  • Organizer: Gordon Research Conference on Meiosis
  • Place of Presentation: New London, NH, USA
  • Year and Date: 2014-06-06 – 2014-06-11
  • Invited
• [Presentation] Control of Rad51 filament formation (2014)
  • Author(s): Shinohara, A.
  • Organizer: Abacam meeting on Mechanism of recombination
  • Place of Presentation: Alicante Spain
  • Year and Date: 2014-05-19 – 2014-05-23
  • Invited
• [Remarks] 大阪大学蛋白質研究所ゲノム～染色体機能研究室ホームページ
  • URL: http://www.protein.osaka-u.ac.jp/genome/Shinohara-HP-index.html
• [Remarks] 大阪大学 蛋白質研究所 ゲノムー染色体機能研究室HP
  • URL: http://www.protein.osaka-u.ac.jp/genome/index.html