| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
I picked up tRNA, a long-lived class of RNAs, to examine whether eukaryotic cells performing asymmetric cell division deliver 'wounded' tRNA molecules asymmetrically like aggregated proteins, and how modified nucleotides derived from degraded tRNA are processed, and tried to establish experimental systems in yeast. I found that 'old' yeast cells from the view of the replicative life span do not show significant difference from young cells in their tRNA localization. I also isolated temperature-sensitive tRNA mutants that lead transient increase of modified nucleotides in amount by degradation of the tRNA molecules and allow analysis of dynamics of the modified nucleotides. Finally, I analyzed molecular mechanism of nuclear import of tRNA, which strongly affects intracellular localization of tRNA, and found that one of the yeast cytosolic Hsp70s, Ssa2p, is a transport carrier of tRNA in the nuclear import.
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