Physiological role of Ufm1 system

• Project/Area Number: 26650012
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Niigata University
• Principal Investigator: Komatsu Masaaki 新潟大学, 医歯学系, 教授 (90356254)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: タンパク質修飾 / Ufm1 / Uba5 / ノックアウトマウス / 遺伝性発達障害 / ユビキチン様修飾システム / 発達障害 / ユビキチン / ユビキチン様分子 / オートファジー / 遺伝子改変マウス

Outline of Final Research Achievements

1.We identified UBA5 as a causative gene for a hereditary developmental disease. 2.We generated knock-in mice harboring the disease-related Uba5 mutation. 3.We developed an experiment system that we can easily detect Ufm1-conjugated proteins, and we identified several novel targets for Ufm1 using this system.

Research Products

• [Int'l Joint Research] University of Helsinki/University of Oulu(Finland)
• [Int'l Joint Research] Wellcome Trust Sanger Institute(英国)
• [Int'l Joint Research] Merck KGaA/Goethe University(Germany)
• [Journal Article] Structural and functional analysis of a novel interaction motif within UFM1-activating enzyme 5 (UBA5) required for binding to ubiquitin-like proteins and ufmylation. (2016)
  • Author(s): Habisov S, Huber J, Ichimura Y, Akutsu M, Rogova N, Loehr F, McEwan DG, Johansen T, Dikic I, Doetsch V, Komatsu M, Rogov VV, Kirkin V.
  • Journal Title: J Biol Chem. Volume: - Issue: 17 Pages: 9025-9041
  • DOI: 10.1074/jbc.m116.715474
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Modification of ASC1 by UFM1 is crucial for ERα transactivation and breast cancer development. (2014)
  • Author(s): Yoo, HM., Kang, SH., Kim, JY., Lee, JE., Seong, MW., Lee, SW., Ka, SH., Sou,YS., Komatsu, M., Tanaka, K., Lee, ST., Noh, DY., Baek, SH., Jeon, YJ., *Chung, CH.
  • Journal Title: Mol. Cell Volume: 56 Issue: 2 Pages: 261-274
  • DOI: 10.1016/j.molcel.2014.08.007
  • Peer Reviewed
• [Presentation] 神経系におけるユビキチン様修飾システムUfm1システムの役割 (2015)
  • Author(s): 石村 亮輔、植村 武文、和栗 聡、田中 啓二、小松 雅明
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-11-30
• [Remarks]
  • URL: http://www.med.niigata-u.ac.jp/bc1/welcome.html
• [Remarks]
  • URL: http://www.helsinki.fi/neurosci/groups/lehesjoki_group.html
• [Remarks] 新潟大学大学院医歯学総合研究科 分子遺伝学講座
  • URL: http://www.med.niigata-u.ac.jp/bc1/welcome.html