• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of new cell field structure technology to elucidate the basic mechanism of eukaryotes

Research Project

Project/Area Number 26650016
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionInstitute of Physical and Chemical Research (2015-2016)
Osaka University (2014)

Principal Investigator

Iwane Atsuko  国立研究開発法人理化学研究所, 生命システム研究センター, ユニットリーダー (30252638)

Research Collaborator ICHINOSE Takako  大阪大学, 生命機能研究科, 招へい研究員 (40776902)
NAGAI Rina  国立研究開発法人理化学研究所, 生命システム研究センター, テクニカルスタッフ (60392049)
OHTA Keisuke  国立研究開発法人理化学研究所, 生命システム研究センター, 客員主管研究員 (00258401)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
KeywordsFIB-SEM / 超微細構造 / 細胞分裂 / シゾン / 三次元再構築 / ダイナミクス / 電子顕微鏡 / 超微細構造解析 / 3次元再構築 / 3次元立体構造 / イメージング
Outline of Final Research Achievements

Microstructural analysis in cells and tissues has been pursued by TEM observation of ultrathin sections prepared by chemical fixation-staining. In recent years As FIB-SEM contributing to the surface processing of metals and ceramics has been used in recent years, we have developed new technologies for application for a 3D-structural analysis of biological sample using its system. We chose " Cyanidioschyzon merolae " which has a minimal simple structure as a eukaryote as a model organism, representative exists cell organelle one by one, and according to the organelles and target molecules linked to the cell division process. We clarified the ultrastructural changes of during the cell mitosis at a whole cell level. By referring to the dynamics by optical microscope, we created a 3D structural model adapted to the time series of cell division and clarified the interaction and shape change among organelles.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (26 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (2 results) (of which Open Access: 2 results) Presentation (21 results) (of which Int'l Joint Research: 5 results,  Invited: 6 results) Book (1 results)

  • [Int'l Joint Research] Marine Biological Laboratory(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Marine Biological Laboratory(米国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] 3D-Visualization of the Precise Location of Symbiotic Organelle Crosstalk Throughout Mitosis in the Primitive Unicellular Eukaryotic Cell, C. Merolae2017

    • Author(s)
      Takako Ichinose, Keisuke Ohta, Atsuko H. Iwane
    • Journal Title

      Biophysical Journal

      Volume: 112 Issue: 3 Pages: 576a-576a

    • DOI

      10.1016/j.bpj.2016.11.3103

    • Related Report
      2016 Annual Research Report
    • Open Access
  • [Journal Article] 3D Microstructural Visualization of the Simplest of Eukaryotic Cell (Cyanidioschyzon Merolae) during Mitosis Process using Several New Microscopic Techniques2016

    • Author(s)
      Atsuko H. Iwane and Keisuke Ohta
    • Journal Title

      Biophysical Journal

      Volume: 110 Issue: 3 Pages: 155-155

    • DOI

      10.1016/j.bpj.2015.11.870

    • Related Report
      2016 Annual Research Report
    • Open Access
  • [Presentation] 先端電子顕微鏡/光学顕微鏡それぞれの特性を生かして細胞分裂過程を三次元微細構造解析する2017

    • Author(s)
      一ノ瀬孝子、岩根敦子
    • Organizer
      生体運動班会議2017
    • Place of Presentation
      神戸国際会議場 (兵庫県神戸市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Construction of the imaging system to elucidate the process by which cytoskeletal proteins acquire function using primitive eukaryotic cell.2017

    • Author(s)
      Takako M. Ichinose and Atsuko H. Iwane
    • Organizer
      Biophysical Society 61st Annual Meeting
    • Place of Presentation
      New Orleans Morial Convention Center (New Orleans, USA)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] D visualization of the precise location of symbiotic organelle crosstalk throughout mitosis in the primitive unicellular eukaryotic cell, C. merolae.2017

    • Author(s)
      Takako M. Ichinose, Keisuke Ohta and Atsuko H. Iwane
    • Organizer
      Biophysical Society 61st Annual Meeting
    • Place of Presentation
      New Orleans Morial Convention Center (New Orleans, USA)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 先端電子顕微鏡により細胞分裂過程を一細胞丸ごとレベルで可視化する2016

    • Author(s)
      岩根敦子
    • Organizer
      医学・生物学系共同利用研究報告会
    • Place of Presentation
      大阪大学ナノバイオロジー棟3Fセミナー室(吹田市)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 3D Microstructual visualization of the simplest of eukaryotic cell(Cyanidioschyzon merolae)during mitosis process using several new microscopic techniques2016

    • Author(s)
      Atsuko H. Iwane and Keisuke Ohta
    • Organizer
      Biophysical Society 60th annual meeting
    • Place of Presentation
      Convention center, Los Angels,CA United States
    • Year and Date
      2016-02-28
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 原始真核生物シゾンの有糸分裂過程を先端顕微鏡と3次元構造解析法で可視化する2016

    • Author(s)
      岩根敦子
    • Organizer
      日本顕微鏡学会第72回学術講演会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 3D-microstructural visualization of the simplest eukaryotic cell during mitosis process using several cutting-edge microscopic techniques.2016

    • Author(s)
      Atsuko H. Iwane
    • Organizer
      1st Cyanidioschyzon merolae Symposium
    • Place of Presentation
      東京大学 理学部2号館(東京都文京区)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] New obvious information obtained from cell organelle 3D-structural models of primitive eukaryote.2016

    • Author(s)
      Atsuko H. Iwane, Rina Nagai, Hikari Mori and Takako M. Ichinose
    • Organizer
      第54回日本生物物理学会大会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 先端電子顕微鏡/光学顕微鏡それぞれの特性を活かして細胞丸ごとレベルでの三次元微細構造解析をおこなうことで明らかになったこと2016

    • Author(s)
      岩根敦子
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜会議センター (神奈川県横浜市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 先端電子顕微鏡/光学顕微鏡それぞれの特性を活かして細胞丸ごとレベルでの三次元微細構造解析をおこなうことで明らかになったこと2016

    • Author(s)
      永井里奈、森ひかり、一ノ瀬孝子、岩根敦子
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜展示ホール (神奈川県横浜市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 宿主細胞内における内部共生した細胞小器官が主導する機能を明らかにする試み2016

    • Author(s)
      一ノ瀬孝子、岩根敦子
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜展示ホール (神奈川県横浜市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新顕微鏡技術の特性を活かし単細胞紅藻シゾンの有糸分裂過程を三次元微細構造解析する2015

    • Author(s)
      岩根敦子
    • Organizer
      第38回日本分子生物学会年会 第88回日本生化学会大会(BMB2015)
    • Place of Presentation
      神戸国際展示場(神戸市)
    • Year and Date
      2015-12-02
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 単細胞紅藻の有糸分裂過程を新顕微鏡技術を活かし三次元微細構造解析する2015

    • Author(s)
      岩根敦子
    • Organizer
      SSSEM研究部会&生理研研究会 合同ワークショップ
    • Place of Presentation
      自然科学研究機構岡崎コンファレンスセンター (岡崎市)
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
  • [Presentation] New obvious information obtained from the cell size and shape during mitosis cycle.2015

    • Author(s)
      Rina Nagai, Keisuke Ohta, Takako M. Ichinose, Hikari Mori and Atsuko H. Iwane
    • Organizer
      日本生物物理学会 第53回年会 (BSJ2015)
    • Place of Presentation
      金沢大学角間キャンパス自然科学本館 (金沢市)
    • Year and Date
      2015-09-14
    • Related Report
      2015 Research-status Report
  • [Presentation] 3D microstructural visualization of mitosis using Focused Ion Beam-Scanning Electron Microscope and 3MV Ultra-High Voltage Electron Microscope Tomography with nanoscale resolution at whole cell level.2015

    • Author(s)
      Atsuko H. Iwane and Keisuke Ohta
    • Organizer
      Gordon Research Conferences-Three Dimensional Electron Microscopy
    • Place of Presentation
      Colby-Sawyer College in New London NH United States
    • Year and Date
      2015-06-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 有糸分裂の三次元微細構造変化を一細胞丸ごと・ナノスケールの分解能で可視化する2015

    • Author(s)
      岩根敦子
    • Organizer
      日本顕微鏡学会第71回学術講演会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      2015-05-15
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Three-dimensional microstructural visualization of mitosis using Focused Ion Beam-Scanning Electron Microscope (FIB-SEM) and 3MV Ultra-High Voltage Electron Microscope (UHVEM) Tomography with nanoscale resolution at whole cell level.2015

    • Author(s)
      Atsuko H. Iwane, Keisuke Ohta
    • Organizer
      59th Annual Meeting Biophysical Society
    • Place of Presentation
      Baltimore Convention Center, Baltimore, Maryland, USA
    • Year and Date
      2015-02-11
    • Related Report
      2014 Research-status Report
  • [Presentation] Single Cell 3D structural analysis2014

    • Author(s)
      岩根敦子
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 集束イオンビーム加工-走査電子顕微鏡を用いて有糸分裂の三次元微細構造変化を一細胞丸ごと・ナノスケールの分解能で可視化する2014

    • Author(s)
      Rina Nagai, Keisuke Ohta, Takako M. Ichinose, Akinobu Togo, Atsuko H. Iwane
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Visualization of cell cycle by three-dimensional FIB-SEM with nanoscale resolution at whole cell level2014

    • Author(s)
      Rina Nagai, Keisuke Ohta, Takako M. Ichinose, Akinobu Togo and Atsuko H. Iwane
    • Organizer
      第52回日本生物物理学会年会(BSJ2014)
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Visualization of cell cycle by three-dimensional FIB-SEM with nanoscale resolution at whole cell level.2014

    • Author(s)
      Atsuko H. Iwane, Keisuke Ohta
    • Organizer
      第37回内藤コンファレンス バイオイメージングがめざすもの
    • Place of Presentation
      ヒルトンニセコビレッジ(北海道ニセコ町)
    • Year and Date
      2014-07-16 – 2014-07-17
    • Related Report
      2014 Research-status Report
  • [Book] Cyanidioschyzon merolae: A New Model Eukaryote for Cell and Organelle Biology2017

    • Author(s)
      Takako M. Ichinose and Atsuko H. Iwane
    • Publisher
      Springer
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2022-02-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi