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Protein NMR method developed for macromolecular complex

Research Project

Project/Area Number 26650018
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionOsaka University

Principal Investigator

Kojima Chojiro  大阪大学, たんぱく質研究所, 准教授 (50333563)

Co-Investigator(Renkei-kenkyūsha) FUJIWARA Toshimichi  大阪大学, 蛋白質研究所, 教授 (20242381)
MATSUKI Yoh  大阪大学, 蛋白質研究所, 助教 (70551498)
SUGIKI Toshihiko  大阪大学, 蛋白質研究所, 助教 (70635698)
FURUITA Kyoko  大阪大学, 蛋白質研究所, 研究員 (30727665)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsNMR / 蛋白質 / 立体構造
Outline of Final Research Achievements

Protein structure can be determined by solution NMR precisely, however, molecular weight is limited to below 100kDa. In this study, NMR method has been developed for protein structure analysis, especially for very big protein beyond 100kDa. Here, double-acquisition method was developed to acquire twice data within 1 scan. This double-acquisition method was combined with 13C direct detection method, and optimized for macromolecular complex. Using these advanced methods, about 70% signals of florigen receptor are assigned.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (15 results)

All 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 8 results,  Open Access: 1 results,  Acknowledgement Compliant: 5 results) Presentation (6 results) (of which Int'l Joint Research: 5 results,  Invited: 6 results)

  • [Int'l Joint Research] IOCB AS CR(チェコ)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Direct Detection of the Mercury-Nitrogen Bond in the Thymine-HgII-Thymine Base-pair with 199Hg NMR Spectroscopy2015

    • Author(s)
      Takenori Dairaku, Kyoko Furuita, Hajime Sato, Jakub Sebera, Daichi Yamanaka, Hiroyuki Otaki, Shoko Kikkawa, Yoshinori Kondo, Ritsuko Katahira, F. Matthias Bickelhaupt, Celia Fonseca Guerra, Akira Ono, Vladimir Sychrovsky, Chojiro Kojima and Yoshiyuki Tanaka
    • Journal Title

      Chemical Communications

      Volume: 51 Issue: 40 Pages: 8488-8491

    • DOI

      10.1039/c5cc02423d

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Physicochemical Nature of Interfaces Controlling Ferredoxin NADP+ Reductase Activity through Its Interprotein Interactions with Ferredoxin2015

    • Author(s)
      Kinoshita Misaki, Kim Ju Yaen, Kume Satoshi, Sakakibara Yukiko, Sugiki Toshihiko, Kojima Chojiro, Kurisu Genji, Ikegami Takahisa, Hase Toshiharu, Kimata-Ariga Yoko, Lee Young-Ho
    • Journal Title

      Biochimica et Biophysica Acta-Bioenergetics

      Volume: 1847 Issue: 10 Pages: 1200-1211

    • DOI

      10.1016/j.bbabio.2015.05.023

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Exploring a DNA sequence for the three-dimensional structure determination of a silver(I)-mediated C-C base pair in a DNA duplex by 1H NMR spectroscopy2015

    • Author(s)
      Takenori Dairaku, Kyoko Furuita, Hajime Sato, Yoshinori Kondo, Chojiro Kojima, Akira Ono and Yoshiyuki Tanaka
    • Journal Title

      Nucleosides, Nucleotides & Nucleic Acids

      Volume: 34 Issue: 12 Pages: 877-900

    • DOI

      10.1080/15257770.2015.1088160

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The crystal structure of the plant small GTPase OsRacl reveals its mode of binding to NADPH oxidase.2015

    • Author(s)
      Kosami KI, Ohki I, Nagano M, Furuita F, Sugiki T, Kawano Y, Kawasaki T, Fujiwara T, Nakagawa A, Shimamoto K, Kojima C
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 289 Issue: 41 Pages: 28569-78

    • DOI

      10.1074/jbc.m114.603282

    • Description
      (in press)(印刷中)
    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Utilization of paramagnetic relaxation enhancements for high-resolution NMR structure determination of a soluble loop-rich protein with sparse NOE distance restraints2015

    • Author(s)
      Kyoko Furuita, Saori Kataoka, Toshihiko Sugiki, Yoshikazu Hattori, Naohiro Kobayashi, Takahisa Ikegami, Kazuhiro Shiozaki, Toshimichi Fujiwara and Chojiro Kojima
    • Journal Title

      J. Biomol. NMR

      Volume: 61 Issue: 1 Pages: 55-64

    • DOI

      10.1007/s10858-014-9882-7

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] ^<1>H, ^<15>N and ^<13>C resonance assignments of the conserved region in the middle domain of S. pombe Sin1 protein.2014

    • Author(s)
      Kataoka S, Furuita K Hattori Y, Kobayashi N, Ikegami T, Shiozaki K, Fujiwara T, Kojima C
    • Journal Title

      Biomolecular NMR Assignments

      Volume: (印刷中) Issue: 1 Pages: 89-92

    • DOI

      10.1007/s12104-014-9550-6

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bacterial effector modulation of host E3 ligase activity suppresses PAMP-triggered immunity in rice.2014

    • Author(s)
      Ishikawa, K., Yamaguchi, K., Sakamoto, K., Yoshimura, S., Inoue, K., Tsuge, S., Kojima, C., Kawasaki, T.
    • Journal Title

      Nature Communications

      Volume: 5 Issue: 1 Pages: 5430-5430

    • DOI

      10.1038/ncomms6430

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Latest approaches for efficient protein production in drug discovery2014

    • Author(s)
      Toshihiko Sugiki, Toshimichi Fujiwara and Chojiro Kojima
    • Journal Title

      Expert Opin. Drug Discov.

      Volume: 9 Issue: 10 Pages: 1189-1204

    • DOI

      10.1517/17460441.2014.941801

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] PRE and PCS techniques utilized for high-resolution protein structure determination2015

    • Author(s)
      Chojiro Kojima
    • Organizer
      The 2015 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Sheraton Waikiki (Honolulu)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] NMR tools developed for drug discovery2015

    • Author(s)
      Chojiro Kojima
    • Organizer
      International Symposium on Structure and Folding of Disease Related Proteins
    • Place of Presentation
      Seoul National University (Seoul)
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Utilization of Paramagnetic NMR techniques for protein structure determination2015

    • Author(s)
      Chojiro Kojima
    • Organizer
      The 6th Asia-Pacific NMR Symposium
    • Place of Presentation
      The Hong Kong University of Science and Technology (Hong Kong)
    • Year and Date
      2015-08-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Paramagnetic NMR techniques utilized for protein structure analysis2015

    • Author(s)
      Chojiro Kojima
    • Organizer
      The 3rd Awaji International Workshop on “Electron Spin Science & Technology: Biological and Materials Science Oriented Applications”
    • Place of Presentation
      The Awaji Yumebutai International Conference Center (Awaji)
    • Year and Date
      2015-06-14
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Drug screening using NMR to regulate protein function2015

    • Author(s)
      Chojiro Kojima
    • Organizer
      The First Trilateral Workshop For Frontier Protein Studies
    • Place of Presentation
      Peking University (Beijing)
    • Year and Date
      2015-04-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Paramagnetic NMR utilized for high-resolution protein structure determination2014

    • Author(s)
      Chojiro Kojima
    • Organizer
      The 5th Japan-Taiwan bilateral NMR symposium
    • Place of Presentation
      Hokkaido University (Sapporo)
    • Year and Date
      2014-09-29 – 2014-09-30
    • Related Report
      2014 Research-status Report
    • Invited

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Published: 2014-04-04   Modified: 2022-02-01  

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