| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Autophagy is a bulk degradation system which is mediated by a unique organelle "autophagosome". It has been few reports on the lipid component of the autophagosome membrane. We have previously developed the unique method for the isolation of autophagosomes using latex beads. Thus, we set out to purify the autophagosomes to clarify the lipid component. We found the suitable condition to collect the high amounts and high purity of autophagosomes. Under the condition, we tried MS analysis, but unfortunately we could not detect any peak of lipids in MS. Hereafter, we will find the more suitable condition, and perform the analysis of lipid component of autophagosomes.
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