| Project/Area Number |
26650022
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
HAKOSHIMA TOSHIO 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (00164773)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | タンパク質 / 構造変化 / 張力 / 構造転移 / 細胞骨格 / 細胞接着 / タンパク質工学 / 蛍光 / 構造生物学 / 生化学 / 細胞生物学 / 分子生物学 / 生物物理学 / 相互作用 / メカノバイオロジー / 分子構造変化 / エネルギー移動 |
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| Outline of Final Research Achievements |
Cells are able to sense mechanical forces. In this study, we focus on α-catenin, which has been elucidated to function as a mechanosensor at adherens junctions of cells. Together with other proteins that are expected to interact with α-catenin in a force-dependent manner, we have analyzed the force-sensing actions of α-catenin and tried to create fusion proteins that are able to show force-dependent luminescence. We have succeeded to show a relationship between the force-sensing properties of α-catenin and conformational changes occurred in the protein molecule under tension. We have also provided molecular basis for further analysis of the force-sensing properties of other protein molecules.
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