Development of a novel protein purification/regulation methodology by using a temperature-sensitive monoclonal antibody.
| Project/Area Number |
26650024
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | モノクローナル抗体 / タンパク質相互作用 / 一本鎖抗体 / サーモジェネティクス / タンパク質間相互作用 |
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| Outline of Final Research Achievements |
We recently developed a monoclonal antibody that shows strong temperature dependence in the binding to the epitope peptide, a carboxyl-terminal sequence of chicken CRY4.
In this study, we tested the possibility to use this epitope peptide as a tag for temperature-dependent affinity purification of the tagged-proteins. We found the binding of the antibody with epitope-tagged glutathione S-transferase (GST) and yellow fluorescent protein (YFP) in a temperature-dependent manner. We also succeeded in the temperature-dependent purification of those proteins.
This technology is the establishment of a new tag and purification methods of proteins, and also it would be applicable in a various fields to the temperature-dependent control of protein localization and function.
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Report
(3 results)
Research Products
(18 results)
