| Project/Area Number |
26650027
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
KASAI Takuma 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (70446516)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 蛋白質 / NMR / アミノ酸選択的安定同位体標識 / 符号化標識法 / ピークフィッティング / レプリカ交換モンテカルロ法 / タンパク質 / アミノ酸選択的安定同位体標識法 |
|---|
| Outline of Final Research Achievements |
Amino-acid selective stable isotope labeling is a powerful method for signal assignment processes for protein analyses with NMR. This method relies on expression systems with suppressed amino-acid scrambling, such as cell-free protein synthesis. The basic idea of this study is to estimate change of isotope labeling ratio derived from the amino-acid scrambling as unknown parameters from NMR spectra. Such approach requires a method for searching huge parameter space. Therefore we developed a parameter-optimization method for analyses of NMR spectra of amino-acid selectively isotope labeled proteins, using replica exchange Monte Carlo. This method also enabled analyses of low signal-to-noise-ratio spectra.
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