Investigation for the oscillatory mechanism of circadian rhythms independent of transcriptional-translational feedback loops using erhythrocyte progenitor cells
| Project/Area Number |
26650033
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
Ohkawa Taeko 名古屋大学, 生命農学研究科, 准教授 (30432230)
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| Research Collaborator |
FURUKAWA Yuko
ISHIGURO Masateru
KOBAYASHI Akane
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 概日リズム / レドックス制御 / ケミカルバイオロジー |
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| Outline of Final Research Achievements |
It has been thought that transcription/translation of clock genes is indispensable for the circadian oscillation. Recently, however, several rhythms were reported to be independent of these processes. In this study, we tried to clarify the mechanism of such oscillations and the relationship between circadian rhythm and intracellular redox regulation. We differentiated erythroid-progenitor cells into enucleated cells, prepared time-series total protein samples, and checked the oxidation state of anti-oxidant protein peroxiredoxin by western analysis. However, the oxidation rhythm, previously reported for human erythrocyte, has not yet been reproduced. To elucidate the relationship between circadian oscillation and intracellular redox regulation, we screened a chemical library containing compounds with prooxidant/ antioxidant activity. We obtained a hit that regulates the circadian phase. Further study will reveal the role of redox regulation in the circadian clock system.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] C-H Activation Generates Period-Shortening Moleculaes That Target Cryptochrome in the Mammalian Circadian Clock.2015
Author(s)
Oshima T, Yamanaka I, Kumar A, Yamaguchi J, Nishiwaki-Okawa T, Muto K, Kawamura R, Hirota T, Yagita K, Irie S, Kay SA, Yoshimura T, Itami K.
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Journal Title
Angew Chem Int Ed
Volume: 54
Issue: 24
Pages: 7193-7197
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] C-H activation generates period shortening molecules targeting Cryptochrome in the mammalian circadian clock2015
Author(s)
Tsuyoshi Oshima, Iori Yamanaka, Anupriya Kumar, Junichiro Yamaguchi, Taeko Nishiwaki-Ohkawa, Kei Muto, Rika Kawamura, Tsuyoshi Hirota, Kazuhiro Yagita, Stephan Irle, Steve A. Kay, Takashi Yoshimura, Kenichiro Itami
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Journal Title
Angewandte Chemie International Edition
Volume: 未定
Related Report
Peer Reviewed
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[Journal Article] Tissue-specific post-translational modification allows functional targeting of thyrotropin2014
Author(s)
Ikegami K, Liao XH, Hoshino Y, Ono H, Ota W, Ito Y, Nishiwaki-Ohkawa T, Sato C, Kitajima K, Iigo M, Shigeyoshi Y, Yamada M, Murata Y, Refetoff S, Yoshimura T
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Journal Title
Cell Reports
Volume: 9
Issue: 3
Pages: 801-809
DOI
Related Report
Peer Reviewed / Open Access
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