| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In buddin yeast, oligosaccharyltransferase generates free oligosaccharides (fOSs) by degrading dolichol-linked oligosaccharides in the lumen of the endoplasmic reticulum. Although it has been known that fOSs are transported into the cytosol and catabolized by the cytosol/vacuolar mannosidase, the molecular mechanism underlying the fOS catabolism remains largely elusive. Here we used non-essential gene knockout collection of budding yeast to screen genes involved in fOS catabolism and found that the knockout of HRD1 results in the two-fold increase in the amounts of fOSs. This result strongly indicate that HRD1 is involved in the catabolism of fOSs.
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