Studies on metabolic pathway of free oligosaccharides generated in the ER lumen
Project/Area Number |
26650040
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Functional biochemistry
|
Research Institution | Kagoshima University (2015) The Institute of Physical and Chemical Research (2014) |
Principal Investigator |
Harada Yoichiro 鹿児島大学, 医歯(薬)学総合研究科, 特任准教授 (80464147)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 小胞体 / アスパラギン結合型糖鎖 / 遊離糖鎖 / 代謝 / 輸送 |
Outline of Final Research Achievements |
In buddin yeast, oligosaccharyltransferase generates free oligosaccharides (fOSs) by degrading dolichol-linked oligosaccharides in the lumen of the endoplasmic reticulum. Although it has been known that fOSs are transported into the cytosol and catabolized by the cytosol/vacuolar mannosidase, the molecular mechanism underlying the fOS catabolism remains largely elusive. Here we used non-essential gene knockout collection of budding yeast to screen genes involved in fOS catabolism and found that the knockout of HRD1 results in the two-fold increase in the amounts of fOSs. This result strongly indicate that HRD1 is involved in the catabolism of fOSs.
|
Report
(3 results)
Research Products
(11 results)