Development of scanning optical microscope capable of the excitation-emission spectral measurement
| Project/Area Number |
26650043
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Tohoku University |
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Principal Investigator |
Shibabta Yutaka 東北大学, 理学(系)研究科(研究院), 准教授 (20300832)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 顕微分光 / 励起スペクトル / １分子分光 |
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| Outline of Final Research Achievements |
Although the fluorescence microscopy has been applied to obtain the fluorescence spectra of a sample, it has not been applied to obtain the excitation spectra. A measurement of an excitation spectrum takes more time than that of an emission spectrum because it requires scanning of the excitation wavelength. I used the photonic-crystal fiber to convert the femto-second pulsed laser light to a white light for the excitation. I developed an optical design in which the excitation laser spots with different wavelengths are focused to different positions of the sample. This optical design enable me to realize a microscope capable of the measurement of both excitation and emissino spectra.
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Report
(3 results)
Research Products
(24 results)
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[Presentation] CHARACTERIZATION OF CHLOROSOMES VARIANTS CONTAINING BACTERIOCHLOROPHYLL c, d, e, OR f IN THE MUTANTS DERIVED FROM THE IDENTICAL STRAIN OF BROWN-COLORED GREEN SULFUR BACTERIUM2014
Author(s)
Jiro Harada, Yutaka Shibata, Misaki Ryono, Ken Yamamoto, Tadashi Mizoguchi, Hitoshi Tamiaki
Organizer
2014 International Conference on Artificial Photosynthesis
Place of Presentation
Awaji City, Hyogo, Japan
Year and Date
2014-11-24 – 2014-11-27
Related Report
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