Development of scanning optical microscope capable of the excitation-emission spectral measurement

• Project/Area Number: 26650043
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Tohoku University
• Principal Investigator: Shibabta Yutaka 東北大学, 理学(系)研究科(研究院), 准教授 (20300832)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 顕微分光 / 励起スペクトル / １分子分光

Outline of Final Research Achievements

Although the fluorescence microscopy has been applied to obtain the fluorescence spectra of a sample, it has not been applied to obtain the excitation spectra. A measurement of an excitation spectrum takes more time than that of an emission spectrum because it requires scanning of the excitation wavelength. I used the photonic-crystal fiber to convert the femto-second pulsed laser light to a white light for the excitation. I developed an optical design in which the excitation laser spots with different wavelengths are focused to different positions of the sample. This optical design enable me to realize a microscope capable of the measurement of both excitation and emissino spectra.

Research Products

• [Journal Article] Structure based modeling of fluorescence kinetics of photosystem II: Relation between its dimeric form and photoregulation (2016)
  • Author(s): A. Mohamed, R. Nagao, T. Noguchi, H. Fukumura, Y. Shibata
  • Journal Title: J. Phys. Chem. B Volume: 120 Issue: 3 Pages: 365-376
  • DOI: 10.1021/acs.jpcb.5b09103
  • Peer Reviewed
• [Journal Article] Scrambled Self-Assembly of Bacteriochlorophylls c and e in Aqueous Triton X-100 Micelles (2014)
  • Author(s): Yoshitaka Saga, Tatsuya Saiki, Naoya Takahashi, Yutaka Shibata, and Hitoshi Tamiaki
  • Journal Title: Photochem. and Photobiol. Volume: 90 Issue: 3 Pages: 552-559
  • DOI: 10.1111/php.12219
  • Peer Reviewed
• [Journal Article] Development of a novel cryogenic microscope with numerical aperture of 0.9 and its application to photosynthesis research (2014)
  • Author(s): Y. Shibata, W. Katoh, T. Chiba, K. Namie, N. Ohnishi, J. Minagawa, H. Nakanishi, T. Noguchi and H. Fukumura
  • Journal Title: Biochim. Biophys. Acta Volume: 1837 Issue: 6 Pages: 880-887
  • DOI: 10.1016/j.bbabio.2014.03.006
  • Peer Reviewed
• [Journal Article] 光合成系の光捕集過程を構造に立脚して理解する：理論と実験の融合で見えてきたこと (2014)
  • Author(s): 柴田 穣
  • Journal Title: 日本物理学会誌 Volume: 69 Pages: 639-643
  • Peer Reviewed
• [Journal Article] 開口数0.9を持つ極低温顕微鏡の開発 (2014)
  • Author(s): 柴田穣，加藤渉，浪江慶祐
  • Journal Title: 分光研究 Volume: 63 Pages: 103-105
• [Presentation] 極低温顕微鏡を用いた緑化途上トウモロコシ生葉の光合成タンパク質構築過程の追跡 (2016)
  • Author(s): 柴田 穣，千葉 智史，福村 裕史
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] Fluorescence spectroscopy of single Photosystem I at liquid nitrogen temperatures (2016)
  • Author(s): Du Ting, Ryo Nagao, Takumi Noguchi, Hiroshi Fukumura, Yutaka Shibata
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] クラミドモナスのステート遷移における光捕集タンパク質の移動を極低温顕微分光法で検証する (2016)
  • Author(s): 伊藤 稚菜，鷲山 研人，福村 裕史，柴田 穣
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] 極低温顕微鏡を用いた緑化途上トウモロコシ生葉の光合成タンパク質前駆体の空間分布の測定 (2015)
  • Author(s): 千葉 知史、福村 裕史、柴田 穣
  • Organizer: 日本生物物理学会第53回年会
  • Place of Presentation: 金沢大学 角間キャンパス
  • Year and Date: 2015-09-13
• [Presentation] Fluorescence spectroscopy of single Photosystem I at liquid nitrogen temperatures (2015)
  • Author(s): Du Ting, Ryo Nagao, Takumi Noguchi, Hiroshi Fukumura, Yutaka Shibata
  • Organizer: 日本生物物理学会第53回年会
  • Place of Presentation: 金沢大学 角間キャンパス
  • Year and Date: 2015-09-13
• [Presentation] 新開発の極低温顕微鏡による光合成研究の新展開 (2015)
  • Author(s): 柴田 穣
  • Organizer: 平成27年度化学系学協会東北大会
  • Place of Presentation: 弘前大学文京キャンパス
  • Year and Date: 2015-09-12
  • Invited
• [Presentation] Two-Photon Fluorescence of Protein-Protected Gold Nanoclusters of Different Sizes (2015)
  • Author(s): Hamza Al-Kindi, Mayu Nakagomi, Rie Kusama, Ahmed Ali, Shinji Kajimoto, Izabela Rzeznicka, Yutaka Shibata, Hiroshi Fukumura
  • Organizer: 第３７回日本光医学・光生物学会年会
  • Place of Presentation: シーガイアコンベンションセンター
  • Year and Date: 2015-07-17
• [Presentation] 植物型光合成タンパク質の高効率かつ柔軟な光捕集 (2015)
  • Author(s): 柴田 穣
  • Organizer: 分子系の複合電子機能第181委員会 第22回研究会
  • Place of Presentation: 大阪大学
  • Year and Date: 2015-07-07
  • Invited
• [Presentation] Refined Compartment Target Analysis of Photosystem II-Enriched Membrane based on Its Structure (2015)
  • Author(s): Ahmed Ali, Ryo Nagao, Takumi Noguchi, Hiroshi Fukumura, Yutaka Shibata
  • Organizer: 27th International Conference on Photochemistry
  • Place of Presentation: International Convention Center Jeju, Jeju, Korea
  • Year and Date: 2015-06-28
  • Int'l Joint Research
• [Presentation] Photo-Physicochemical Processes of Gold Nanoclusters Synthesized in Bovine Serum Albumin (2015)
  • Author(s): Hamza Al-Kindi, Ahmed Ali, Shinji Kajimoto, Izabela Rzeznicka, Yutaka Shibata, Hiroshi Fukumura
  • Organizer: 27th International Conference on Photochemistry
  • Place of Presentation: International Convention Center Jeju, Jeju, Korea
  • Year and Date: 2015-06-28
  • Int'l Joint Research
• [Presentation] 緑色イオウ光合成細菌Chlorobaculum limnaeum RK-j-1株の変異体から単離精製した色素の異なるクロロソームの比較評価 (2015)
  • Author(s): 原田 二朗、柴田 穣、溝口 正、漁野 岬、寺村 美里、山本 健、民秋 均
  • Organizer: 日本化学会第95春季年会
  • Place of Presentation: 日本大学
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] ピコ秒時間分解蛍光分光による極地に生育する蘚類および藻類の乾燥耐性の分子機構の研究 (2015)
  • Author(s): 柴田 穣、谷山 航一朗、Ahmed Ali、小杉 真貴子、福村 裕史
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] CHARACTERIZATION OF CHLOROSOMES VARIANTS CONTAINING BACTERIOCHLOROPHYLL c, d, e, OR f IN THE MUTANTS DERIVED FROM THE IDENTICAL STRAIN OF BROWN-COLORED GREEN SULFUR BACTERIUM (2014)
  • Author(s): Jiro Harada, Yutaka Shibata, Misaki Ryono, Ken Yamamoto, Tadashi Mizoguchi, Hitoshi Tamiaki
  • Organizer: 2014 International Conference on Artificial Photosynthesis
  • Place of Presentation: Awaji City, Hyogo, Japan
  • Year and Date: 2014-11-24 – 2014-11-27
• [Presentation] 極低温顕微鏡を用いた緑化途上トウモロコシ生葉の光合成タンパク質前駆体の空間分布の測定 (2014)
  • Author(s): 千葉 知史、福村 裕史、柴田 穣
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Target analysis of the photosystem II-enriched membrane: The effect of oxidizing agent on the fluorescence quenching in PSII (2014)
  • Author(s): Ahmed Ali, Ryo Nagao, Takumi Noguchi, Hiroshi Fukumura, Yutaka Shibata
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 光合成蛋白質と金属ナノ粒子による水素発生人工光合成ナノデバイスの開発 (2014)
  • Author(s): 田原 一輝、河原 弘典、浪江 慶祐、井上 名津子、長尾 遼、加藤 祐樹、鞆 達也、柴田 穣、福村 裕史、菓子野 康浩、野口 巧
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 開口数0.9の極低温顕微鏡の開発と光合成タンパク質の1分子分光への応用 (2014)
  • Author(s): 柴田 穣
  • Organizer: 日本分析化学会 第６３年会特別シンポジウム
  • Place of Presentation: 広島大学
  • Year and Date: 2014-09-17 – 2014-09-19
  • Invited
• [Presentation] 新開発の開口数0.9の極低温光学顕微鏡による細胞内光合成タンパク質の不均一分布測定 (2014)
  • Author(s): 柴田 穣、千葉 知史、福村 裕史
  • Organizer: 第３６回日本光医学・光生物学会年会
  • Place of Presentation: 大阪大学
  • Year and Date: 2014-07-25 – 2014-07-26
• [Presentation] 理論と実験の融合で見えてきた植物型光合成の超高速光反応 (2014)
  • Author(s): 柴田 穣
  • Organizer: 第１１回AMO討論会
  • Place of Presentation: 大阪大学
  • Year and Date: 2014-06-06 – 2014-06-07
  • Invited