Molecular dynamics of RNA silencing mechanism in cell
| Project/Area Number |
26650047
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Sasaki Hiroshi 東京大学, 分子細胞生物学研究所, 助教 (30611454)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | RNA / 1分子イメージング / RNAサイレンシング / 核酸 / RISC / Argonaute / 生物物理学 / 全反射顕微鏡 |
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| Outline of Final Research Achievements |
RNA silencing, such as RNA interference and gene silencing by microRNAs, is one of the fundamental gene regulation mechanisms and universally conserved in eukaryotes. The RNA-induced silencing complex, which is composed of an Argonaute protein and a single-stranded short RNA, plays a central role in this system. However, the detailed mechanisms of RISC assembly as well as target recognition and cleavage remained unclear. By single-molecule imaging approach using total-internal reflection microscopy, here I revealed the fundamental steps in the assembly and target cleavage of RISCs.
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Report
(3 results)
Research Products
(8 results)
