| Project/Area Number |
26650047
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
Sasaki Hiroshi 東京大学, 分子細胞生物学研究所, 助教 (30611454)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | RNA / 1分子イメージング / RNAサイレンシング / 核酸 / RISC / Argonaute / 生物物理学 / 全反射顕微鏡 |
|---|
| Outline of Final Research Achievements |
RNA silencing, such as RNA interference and gene silencing by microRNAs, is one of the fundamental gene regulation mechanisms and universally conserved in eukaryotes. The RNA-induced silencing complex, which is composed of an Argonaute protein and a single-stranded short RNA, plays a central role in this system. However, the detailed mechanisms of RISC assembly as well as target recognition and cleavage remained unclear. By single-molecule imaging approach using total-internal reflection microscopy, here I revealed the fundamental steps in the assembly and target cleavage of RISCs.
|
