| Project/Area Number |
26650048
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | 凍結保護 / バイオ医薬品添加剤 / コールドチェイン / 分子盾効果 / 天然変性タンパク質 / 医薬品添加剤 / 国内優先権による出願 / 国際特許出願 / バイオ医薬品安定化剤 / 凍結保護剤 / 再生・細胞医療 / 原著論文発表 / 医療応用 / 国内優先権に基づく出願 |
|---|
| Outline of Final Research Achievements |
Intrinsically disordered proteins (IDPs), polypeptides that lack a stable compact structure, are known to exert the two major molecular functions, “fly-casting mechanism” and “coupled folding and binding”. Plant stress-responsible protein dehydrins and silkworm sericins are the examples of IDPs. These exert cryoprotective activity against enzymes and cultivated cells, respectively. If IDP’s molecular functions are originated from their physical properties, similar functions may also exist in human-genome derived IDPs.
Based on the concept, we started to screen several human IDPs using cryoprotective activity against the model enzyme lactose dehydrogenase as an index. Finally, we succeeded in finding a 20 aa IDP peptide that showed cryoprotective activity against NHDF cell.
|
