| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
This research is aimed at creating non-natural interaction between proteins based on genetically modified natural proteins carrying random mutations. Initially we used artificial pentapeptide repeat proteins (PRP) for templates. However, RNA/DNA of PRP, recovered from displayed complex including template RNA/DNA and expressed protein, expresses almost insoluble proteins. Next we tried new templates, E. coli YncE and DIX. The former still yielded insoluble proteins but the latter produced relatively highly soluble ones. The selected DIX proteins after CIS-display generally showed higher affinity compared to the wild-type.
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