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Visualization of biological phenomena by the second harmonic generation imaging using the next generation dye.

Research Project

Project/Area Number 26650052
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionKeio University

Principal Investigator

Nuriya Mutsuo  慶應義塾大学, 医学部, 講師 (60453544)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords光第二高調波発生 / 2光子顕微鏡 / SHG / イメージング / アストロサイト / 2光子顕微鏡 / グリア細胞
Outline of Final Research Achievements

One of the two-photon imaging techniques, called the second harmonic generation (SHG) imaging, can visualize unique biological phenomena because of its specific requirements for signal generation. In this study, we attempted to develop an SHG-specific dye to overcome the limitations of SHG imaging associated with uses of currently available general fluorescent dyes. We have successfully developed a non-fluorescent SHG specific dye, Ap3, and realized pure SHG imaging and true multimodal two-photon imaging using this dye. This SHG-specific dye should greatly facilitate the applications of SHG imaging in biological researches.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (8 results)

All 2016 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (3 results) (of which Invited: 2 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Multimodal two-photon imaging using a second harmonic generation-specific dye2016

    • Author(s)
      Mutsuo Nuriya, Shun Fukushima, Atsuya Momotake, Takanori Shinotsuka, Masato Yasui & Tatsuo Arai
    • Journal Title

      Nature Communications

      Volume: - Issue: 1 Pages: 11557-11557

    • DOI

      10.1038/ncomms11557

    • NAID

      120007135505

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Involvement of astrocytes in neurovascular communication2016

    • Author(s)
      Nuriya M, Hirase, H.
    • Journal Title

      Progress in Brain Research

      Volume: in press Pages: 41-62

    • DOI

      10.1016/bs.pbr.2016.02.001

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Sustained Downregulation of β-Dystroglycan and Associated Dysfunctions of Astrocytic Endfeet in Epileptic Cerebral Cortex.2014

    • Author(s)
      Gondo A, Shinotsuka T, Morita A, Abe Y, Yasui M, and Nuriya M.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 289 Issue: 44 Pages: 30279-30288

    • DOI

      10.1074/jbc.m114.588384

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 蛍光だけじゃない!多光子現象を利用した新規顕微鏡法2015

    • Author(s)
      塗谷 睦生
    • Organizer
      第88回日本薬理学会年会
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2015-03-20
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 2光子顕微鏡の神経薬理学研究への応用2014

    • Author(s)
      塗谷 睦生
    • Organizer
      第22回創薬薬理フォーラム
    • Place of Presentation
      日本薬学会長井記念ホール(東京都・渋谷区)
    • Year and Date
      2014-09-26
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Sustained downregulation of β-dystroglycan in astrocytic endfeet in epileptic cerebral cortex.2014

    • Author(s)
      Gondo A, Shinotsuka T, Morita A, Abe Y, Yasui M and Nuriya M.
    • Organizer
      Neuro 2014, 日本神経科学会大会
    • Place of Presentation
      パシフィコ横浜(神奈川・横浜)
    • Year and Date
      2014-09-13
    • Related Report
      2014 Research-status Report
  • [Remarks] 塗谷グループのホームページ

    • URL

      http://user.keio.ac.jp/~aa606547/homepage.html

    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] 光第二高調波発生化合物、光第二高調波発生色素組成物及び細胞検査方法2014

    • Inventor(s)
      塗谷睦生、安井正人、新井達郎、百武篤也、福嶋瞬
    • Industrial Property Rights Holder
      塗谷睦生、安井正人、新井達郎、百武篤也、福嶋瞬
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-05-23
    • Related Report
      2014 Research-status Report
    • Overseas

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Published: 2014-04-04   Modified: 2017-05-10  

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