| Project/Area Number |
26650057
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Hamamatsu Photonics K.K. |
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Principal Investigator |
Kimura Yuji 浜松ホトニクス株式会社, 中央研究所, 専任部員 (90713371)
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| Research Collaborator |
KAZAMI Sayaka 浜松ホトニクス株式会社, 中央研究所, 研究員 (80727991)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 生体膜 / イオン駆動型分子モーター / 1分子計測（SMD） / マイクロ・ナノデバイス / ナノバイオ / イオン駆動型モーター / 呼吸鎖複合体I |
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| Outline of Final Research Achievements |
The purpose of this research is to drive FoF1-ATP synthase with optical illumination. For this purpose, 1) we developed an evaluation and manipulation system for FoF1-ATP synthase by using microfluidics device. The device enabled us to image proton pumping activity of respiratory chain complex I and FoF1-ATP synthase embedded on a membrane of a large-sized protoplast from E-coli, in real time. 2) To drive the ATP-synthase activity, the membrane of the large-sized E-coli protoplast was illuminated with evanescent field. The synthesized ATP was monitored with luciferin/luciferase system. In the observation period (~10 min), however, the activity was not detected. It suggests more detailed analyses on electro-chemical potential are needed.
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