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Analysis of higher-order structures of mitochondria DNA using the enChIP technology

Research Project

Project/Area Number 26650059
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionOsaka University

Principal Investigator

Fujii Hodaka  大阪大学, 微生物病研究所, 准教授 (30302665)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsミトコンドリア / 遺伝子座特異的クロマチン免疫沈降法 / クロマチン / enChIP / ミトコンドリアDNA / TAL / CRISPR
Outline of Final Research Achievements

Genetic information in mammalian cells is separately stored in two organelles, the nucleus and the mitochondria. Extensive analyses have been done to elucidate chromatin structures and transcription factors of the nuclear DNA, whereas only limited information is available on higher order structures and transcription mechanisms of mitochondria DNA (mtDNA). In this study, we aimed to isolate transcriptional regulatory regions of mtDNA, which retains molecular interactions, using the engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) technology to perform non-biased identification of molecules (proteins, RNAs, and others) associated with the region. Subsequently, their roles in regulation of functions of mtDNA such as gene expression will be explored in the hope to elucidate higher-order structures of mtDNA chromatin and gain mechanistic insight on transcriptional regulation of genes on mtDNA.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2016 2015 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 5 results,  Acknowledgement Compliant: 5 results) Remarks (1 results)

  • [Journal Article] Biochemical Analysis of Genome Functions Using Locus-Specific Chromatin Immunoprecipitation Technologies.2016

    • Author(s)
      Fujita T, Fujii H.
    • Journal Title

      Gene Regul Syst Bio

      Volume: 10 Pages: 1-9

    • DOI

      10.4137/grsb.s32520

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins.2016

    • Author(s)
      Fujita T, Yuno M, Fujii H.
    • Journal Title

      Genes Cells

      Volume: 21 Issue: 4 Pages: 370-377

    • DOI

      10.1111/gtc.12341

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Isolation of Specific Genomic Regions and Identification of Associated Molecules by enChIP.2016

    • Author(s)
      Fujita T, Fujii H.
    • Journal Title

      J Vis Exp

      Volume: 107 Issue: 107

    • DOI

      10.3791/53478

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Isolation of Specific Genomic Regions and Identification of Their Associated Molecules by Engineered DNA-Binding Molecule-Mediated Chromatin Immunoprecipitation (enChIP) Using the CRISPR System and TAL Proteins.2015

    • Author(s)
      Fujita T, Fujii H.
    • Journal Title

      Int J Mol Sci

      Volume: 16 Issue: 9 Pages: 21802-21812

    • DOI

      10.3390/ijms160921802

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research.2015

    • Author(s)
      Fujita T, Fujii H.
    • Journal Title

      Int J Mol Sci

      Volume: 16 Issue: 10 Pages: 23143-23164

    • DOI

      10.3390/ijms161023143

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Remarks] 藤井研究室ホームページ

    • URL

      http://www.biken.osaka-u.ac.jp/lab/microimm/fujii/index.html

    • Related Report
      2015 Annual Research Report 2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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