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Direct reprograming to neutrophil-like cells by the compound 2057 that induces lobulated nuclei

Research Project

Project/Area Number 26650062
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionKumamoto University

Principal Investigator

TANI Tokio  熊本大学, 自然科学研究科, 教授 (80197516)

Project Period (FY) 2014-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywords分葉核 / 好中球 / 化合物 / チューブリン / ラミン / HeLa細胞 / 核分葉化 / リプログラミング / Protein Kinase C / 放線菌培養上清
Outline of Final Research Achievements

We identified the compound 2057 that induced the formation of the lobulated nuclei, a typical morphology of neutrophil nuclei, in HeLa cells. In this study, we tested the possibility that the compound 2057 triggers the direct reprograming of HeLa cells to neutrophil-like cells. After the treatment with 2057, we could not detect the expression of the surface antigens specific to the neutrophil cells, although enhanced cell motility was observed in HeLa cells. Our results suggest that 2057 activates Protein Kinase C, leading to the aberrant polymerization of the tubulin and delocalization of Lamin A/C, and induces the lobulation of the nuclei in HeLa cells.

Report

(2 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (6 results)

All 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (5 results)

  • [Journal Article] Involvement of satellite I RNA associating with Aurora B in regulation of chromosome segregation2014

    • Author(s)
      T.Ideue, Y. Cho, K. Nishimura and T. Tani
    • Journal Title

      Genes to Cells

      Volume: 19 Issue: 6 Pages: 528-538

    • DOI

      10.1111/gtc.12149

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] HeLa 細胞核の分葉化を誘導する化合物の同定と解析2014

    • Author(s)
      平田久峰、五十嵐雅之、谷時雄
    • Organizer
      第13回核ダイナミクス研究会
    • Place of Presentation
      安芸グランドホテル(広島県廿日市市)
    • Year and Date
      2014-12-15 – 2014-12-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 生殖細胞特異的構造体ヌアージュの形成を阻害する天然化合物の スクリーニング2014

    • Author(s)
      竹下友佳子、石川聡美、五十嵐雅之、Ramesh S. Pillai、谷時雄
    • Organizer
      第13回核ダイナミクス研究会
    • Place of Presentation
      安芸グランドホテル(広島県廿日市市)
    • Year and Date
      2014-12-15 – 2014-12-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Characterization of compounds that induce formation of lobulated nuclei in HeLa cells2014

    • Author(s)
      Hisataka Hirata, Masayuki Igarashi, Tokio Tani
    • Organizer
      2nd International Symposium for ISMCBC
    • Place of Presentation
      ホテルグリーンピア南阿蘇(熊本県阿蘇郡南阿蘇)
    • Year and Date
      2014-11-30 – 2014-12-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] HeLa 細胞核の分葉化を誘導する化合物 Teleocidin A1 の解析2014

    • Author(s)
      平田久峰、五十嵐雅之、谷時雄
    • Organizer
      第37回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Polycomb group body の形成に影響を与える化合物のスクリーニングと解析2014

    • Author(s)
      佐堂晃太、平田久峰、田中千晶、五十嵐雅之、谷時雄
    • Organizer
      日本ケミカルバイオロジー学会第八回年会
    • Place of Presentation
      東京医科歯科大鈴木章夫記念講堂(東京都文京区)
    • Year and Date
      2014-06-19 – 2014-06-21
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2016-06-03  

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