Direct reprograming to neutrophil-like cells by the compound 2057 that induces lobulated nuclei
| Project/Area Number |
26650062
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Kumamoto University |
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Principal Investigator |
TANI Tokio 熊本大学, 自然科学研究科, 教授 (80197516)
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| Project Period (FY) |
2014-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 分葉核 / 好中球 / 化合物 / チューブリン / ラミン / HeLa細胞 / 核分葉化 / リプログラミング / Protein Kinase C / 放線菌培養上清 |
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| Outline of Final Research Achievements |
We identified the compound 2057 that induced the formation of the lobulated nuclei, a typical morphology of neutrophil nuclei, in HeLa cells. In this study, we tested the possibility that the compound 2057 triggers the direct reprograming of HeLa cells to neutrophil-like cells. After the treatment with 2057, we could not detect the expression of the surface antigens specific to the neutrophil cells, although enhanced cell motility was observed in HeLa cells. Our results suggest that 2057 activates Protein Kinase C, leading to the aberrant polymerization of the tubulin and delocalization of Lamin A/C, and induces the lobulation of the nuclei in HeLa cells.
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Report
(2 results)
Research Products
(6 results)
