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Identification of specific protein composes "fenestration" of glomerular endothelial cells

Research Project

Project/Area Number 26650065
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionJuntendo University

Principal Investigator

ONDA Kisara  順天堂大学, 医学部, 非常勤助教 (60465044)

Co-Investigator(Renkei-kenkyūsha) INOSHITA Hiroyuki  順天堂大学, 医学部, 准教授 (80646117)
Research Collaborator KUSABA Gaku  順天堂大学, 医学部
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords糸球体内皮細胞 / fenestration / 質量分析 / 新規蛋白質の同定 / カベオラ / 透過型電子顕微鏡 / 新規マーカーの発見 / IgA腎症
Outline of Final Research Achievements

Renal dysfunction means declining of glomerular filtration which consists of glomerular basement membrane, podocyte slit diaphragm, and glomerular endothelial cell (GEnC) fenestrations. In this study, we aimed to identify specific proteins compose the fenestration. First, morphology of ultrastructure of human GEnC including fenestration was observed using electroscopy. To isolate GEnC from mice glomeruli, GEnC was cultured from Immortomouse glomeruli and purified by positive selection using anti-CD31 antibody. The selected GEnC was analyzed by FACS. Mass spectrometry should be done in next.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (1 results)

All 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results)

  • [Journal Article] Properdin has an ascendancy over factor H regulation in complement-mediated renal tubular damage2014

    • Author(s)
      長町誠嗣、大澤勲、佐藤信之、他7名
    • Journal Title

      BMC Nephrol

      Volume: 15 Issue: 1 Pages: 82-82

    • DOI

      10.1186/1471-2369-15-82

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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