| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Outline of Final Research Achievements |
In this project, we aimed to develop optogenetic tools to selectively activate a specific pathway of biochemical signaling in synapses. These tools enable us to identify the essential pathways and signaling molecules required for the induction of long-term potentiation. By far, we have developed a prototype photo-activatable CaMKII (paCaMKII), and found that the insertion of LOV2 domain into CaMKII decreases the folding efficiency and induces aggregation of paCaMKII. Therefore, we developed a method to improve the folding efficiency and expression pattern of signaling molecule. An application of this method to paCaMKII also improved the expression pattern and dynamic range of activity in HeLa cells.
|
