Physiological significance of the inter-chromosomal glue that loosely clusters mitotic chromosomes

• Project/Area Number: 26650070
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Takagi Masatoshi 国立研究開発法人理化学研究所, 今本細胞核機能研究室, 専任研究員 (60324779)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 分裂期染色体 / Ki-67 / AID / 染色体安定性 / 染色体間グルー / 微小核

Outline of Final Research Achievements

Observation of chromosome dynamics at the early stage of mitosis in human cultured cells revealed that most chromosomes behave as a gently consolidated group. In this study, we investigated the possibility that the Ki-67 antigen might function as "inter-chromosomal glue" which promotes the behavior of whole mitotic chromosomes in a group. In addition, we proposed a possibility that the inter-chromosomal glue might contribute for the maintenance of chromosome stability via raising the probability that all segregated chromosomes in anaphase are stored in one nucleus in telophase.

Research Products

• [Journal Article] ELYS regulates the localization of LBR by modulating its phosphorylation state. (2017)
  • Author(s): Mimura Y, Takagi M, Clever M, Imamoto N.
  • Journal Title: J Cell Sci. Volume: 129(22) Issue: 22 Pages: 4200-4212
  • DOI: 10.1242/jcs.190678
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prichromosomal protein Ki67 supports mitotic chromosome architecture. (2016)
  • Author(s): Takagi M, Natsume T, Kanemaki MT, Imamoto N.
  • Journal Title: Genes to Cells Volume: 21(10) Issue: 10 Pages: 1113-1124
  • DOI: 10.1111/gtc.12420
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ki-67 is a PP1-interacting protein that organizes the mitotic chromosome periphery (2014)
  • Author(s): Booth, D., Takagi, M., Sanchez-Pulido, L., Petfalski, E., Vargiu, G., Samejima, K., Imamoto, N., Ponting, C., Tollervey, D., Earnshaw, W., and Vagnerelli, P.
  • Journal Title: eLife Volume: 27;3
  • DOI: 10.7554/elife.01641
  • Peer Reviewed
• [Journal Article] Ki67 antigen contributes to the timely accumulation of protein phosphatase 1γ on anaphase chromosomes. (2014)
  • Author(s): Takagi M, Nishiyama Y, Taguchi A, Imamoto N.
  • Journal Title: J Biol Chem. Volume: 15;289(33) Issue: 33 Pages: 22877-87
  • DOI: 10.1074/jbc.m114.556647
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Analytic 3D imaging of mammalian nucleus at nanoscale using coherent x-rays and optical fluorescence microscopy. (2014)
  • Author(s): Song C, Takagi M, Park J, Xu R, Gallagher-Jones M, Imamoto N, Ishikawa T.
  • Journal Title: Biophys J. Volume: 107(5) Issue: 5 Pages: 1074-81
  • DOI: 10.1016/j.bpj.2014.07.028
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] The prichromosomal protein Ki67 supports mitotic chromosome architecture. (2016)
  • Author(s): Takagi M, Natsume T, Kanemaki MT, Imamoto N.
  • Organizer: 第３９回日本分子生物学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] Exploration of the Histone Code Read by the LR Domain of Ki67 (2015)
  • Author(s): 高木昌俊、今本尚子
  • Organizer: 第６７回日本細胞生物学会
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Year and Date: 2015-07-01
• [Presentation] Ki67抗原が分裂期表層領域にPCFを樹立する分子機構 (2014)
  • Author(s): 高木昌俊、今本尚子
  • Organizer: 第３２回染色体ワークショップ、第１３回核ダイナミクス研究会（合同）
  • Place of Presentation: 広島
  • Year and Date: 2014-12-15 – 2014-12-17