Oocyte-specific spindle scaling

• Project/Area Number: 26650072
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Kitajima Tomoya 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, チームリーダー (00376641)
• Co-Investigator(Renkei-kenkyūsha): Kyogoku Hirohisa 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, 訪問研究員 (20726038)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 卵母細胞 / 紡錘体 / 染色体分配 / 減数分裂

Outline of Final Research Achievements

This study aimed to reveal the mechanisms and roles of spindle scaling in oocytes. Four-dimensional live analysis of mouse oocytes following artificial cytoplasmic increase or decrease demonstrated that spindle volume is influenced by cytoplasmic volume. Further investigation of the functionality of the spindles indicated that a larger cytoplasm lowers the spindle functionality and increases the frequency of chromosome segregation errors. These results suggested that the large cytoplasm of oocytes is a cell-intrinsic feature that predisposes to chromosome segregation errors (Kyogoku & Kitajima 2017 Dev Cell).

Research Products

• [Journal Article] Bayesian Inference of Forces Causing Cytoplasmic Streaming in Caenorhabditis elegans Embryos and Mouse Oocytes. (2016)
  • Author(s): Niwayama R., Nagao H., Kitajima T. S., Hufnagel L., Shinohara K., Higuchi T., Ishikawa T., Kimura A.
  • Journal Title: PLoS ONE Volume: 11 Issue: 7 Pages: e0159917-e0159917
  • DOI: 10.1371/journal.pone.0159917
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Super-resolution mapping of neuronal circuitry using an index optimized clearing agent (2016)
  • Author(s): Meng-Tsen Ke, Yasuhiro Nakai, Satoshi Fujimoto, Rie Takayama, Shuhei Yoshida, Tomoya S. Kitajima, Makoto Sato, Takeshi Imai
  • Journal Title: Cell Reports Volume: 14 Issue: 11 Pages: 2718-2732
  • DOI: 10.1016/j.celrep.2016.02.057
  • Peer Reviewed / Open Access
• [Journal Article] Inherent instability of correct kinetochore-microtubule attachments during meiosis I in oocytes. (2015)
  • Author(s): Yoshida, S., Kaido, M., and Kitajima, T.S.
  • Journal Title: Developmental Cell Volume: 33 Issue: 5 Pages: 589-602
  • DOI: 10.1016/j.devcel.2015.04.020
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 減数分裂における染色体分配の制御機構とエラー (2015)
  • Author(s): 北島智也
  • Journal Title: 細胞 Volume: 47 Pages: 17-20
• [Journal Article] 卵母細胞の染色体分配異常 (2015)
  • Author(s): 吉田周平・北島智也
  • Journal Title: 細胞工学 Volume: 34 Pages: 1063-1066
• [Journal Article] Meikin is a conserved regulator of meiosis-I-specific kinetochore function (2015)
  • Author(s): Kim, J., Ishiguro, K., Nambu, A., Akiyoshi, B., Yokobayashi, S., Kagami, A., Ishiguro, T., Pendas, A.M., Takeda, N., Sakakibara, Y., Kitajima, T.S., Tanno, Y., Sakuno, T., and Watanabe, Y.
  • Journal Title: Nature Volume: 517 Issue: 7535 Pages: 466-471
  • DOI: 10.1038/nature14097
  • Peer Reviewed
• [Journal Article] Multiple requirements of PLK1 during mouse oocyte maturation. (2015)
  • Author(s): Solc P, Kitajima TS, Yoshida S, Brzakova A, Kaido M, Baran V, Mayer A, Samalova P, Motlik J, Ellenberg J.
  • Journal Title: PLoS One Volume: 10 Issue: 2 Pages: e0116783-e0116783
  • DOI: 10.1371/journal.pone.0116783
  • Peer Reviewed / Open Access
• [Journal Article] Inherent instability of correct kinetochore-microtubule attachments during meiosis I in oocytes (2015)
  • Author(s): Yoshida S, Kaido M, Kitajima TS.
  • Journal Title: Developmental Cell Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 卵母細胞における染色体分配と老化によるエラー (2014)
  • Author(s): 北島智也
  • Journal Title: 実験医学 Volume: 32 Pages: 865-869
• [Journal Article] Nucleolus precursor body (NPB): A distinct structure in mammalian oocytes and zygotes (2014)
  • Author(s): Kyogoku H, Kitajima TS, Miyano T
  • Journal Title: Nucleus Volume: 5(6) Issue: 6 Pages: 493-498
  • DOI: 10.4161/19491034.2014.990858
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 卵母細胞では動原体が紡錘体の二極化に必要である (2017)
  • Author(s): 吉田周平・北島智也
  • Organizer: 第34回染色体ワークショップ・第15回核ダイナミクス研究会
  • Place of Presentation: かずさアカデミアホール(千葉県)
  • Invited
• [Presentation] The causes of aneuploidy in eggs (2017)
  • Author(s): Kitajima T.S.
  • Organizer: RIKEN Epigenetics 2017
  • Place of Presentation: 理研つくば研究所(茨城）
  • Invited
• [Presentation] 卵子における染色体数異常の細胞生物学的な要因 (2017)
  • Author(s): 北島智也
  • Organizer: 第23回ホルモン療法を考える神奈川県医師の会
  • Place of Presentation: ホテルキャメロットジャパン（神奈川県）
  • Invited
• [Presentation] 卵子の染色体数異常の原因～マウス卵母細胞のライブイメージングを用いた研究から (2016)
  • Author(s): 北島智也
  • Organizer: 日本A-PART学術講演会2016（招待講演）
  • Place of Presentation: 品川インターシティホール（東京）
  • Year and Date: 2016-03-20
  • Invited
• [Presentation] Intrinsic and age-related sources of aneuploidy in eggs (2016)
  • Author(s): Kitajima, T.S.
  • Organizer: International Symposium on Epigenome Dynamics and Regulation in Germ Cells（招待講演)
  • Place of Presentation: 京都大学百周年時計台記念館（京都）
  • Year and Date: 2016-02-18
  • Int'l Joint Research / Invited
• [Presentation] 卵子の染色体数異常の原因 (2016)
  • Author(s): 北島智也
  • Organizer: ナースのためのART医学セミナー2016年
  • Place of Presentation: 六本木アカデミーヒルズ49（東京都）
  • Invited
• [Presentation] 老化にともなう卵子の染色体数異常の原因 (2016)
  • Author(s): 北島智也
  • Organizer: 第68回日本細胞生物学会大会・第11回日本ケミカルバイオロジー学会 合同大会
  • Place of Presentation: 京都テルサ（京都府）
  • Invited
• [Presentation] 卵子の染色体数異常の原因 (2016)
  • Author(s): 北島智也
  • Organizer: 第38回中部生殖医学会学術集会
  • Place of Presentation: アスト津（三重県）
  • Invited
• [Presentation] Effects of oocyte size on chromosome segregation during meiosis (2016)
  • Author(s): Kyogoku H. and Kitajima T.S.
  • Organizer: Meiosis: Gordon Research Conference
  • Place of Presentation: Colby-Sawyer College (米国New London)
  • Int'l Joint Research / Invited
• [Presentation] 卵母細胞の巨大な細胞サイズの意義 (2016)
  • Author(s): 北島智也
  • Organizer: 第18回京都大学生命科学研究科シンポジウム
  • Place of Presentation: 京都大学(京都府)
  • Int'l Joint Research / Invited
• [Presentation] Oocytes - why so error-prone? (2016)
  • Author(s): 北島智也
  • Organizer: 国立遺伝学研究所 セミナー
  • Place of Presentation: 国立遺伝学研究所(静岡県)
  • Invited
• [Presentation] Large cytoplasm predisposes oocytes to chromosome segregation errors (2016)
  • Author(s): Kitajima T.S.
  • Organizer: Conference Pigmod and Czechoslovak Biological Society: From reproductive biology to large animal models of human diseases
  • Place of Presentation: Institute of Animal Physiology and Genetics(チェコLibechov)
  • Int'l Joint Research / Invited
• [Presentation] 卵子の染色体数異常の原因 (2016)
  • Author(s): 北島智也
  • Organizer: 第６１回日本生殖医学会学術講演会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Invited
• [Presentation] 卵母細胞がエラーを起こしやすい原因 (2016)
  • Author(s): 北島智也
  • Organizer: 第３７回インターゲノミクスセミナー「染色体分配システム研究の最前線」
  • Place of Presentation: 神戸大学（兵庫県）
  • Invited
• [Presentation] 卵母細胞がエラーを起こしやすい原因 (2016)
  • Author(s): 北島智也
  • Organizer: 第４回公開シンポジウム 新学術領域会議「生殖細胞のエピゲノムダイナミクスとその制御」「ステムセルエイジングから解明する疾患原理」
  • Place of Presentation: 三島市民文化会館(静岡県)
  • Invited
• [Presentation] 卵子の染色体数異常の原因 (2016)
  • Author(s): 北島智也
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Invited
• [Presentation] 細胞動態のイメージングと定量 (2016)
  • Author(s): 北島智也
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Invited
• [Presentation] Intrinsic and age-related sources of aneuploidy in eggs (2015)
  • Author(s): Kitajima, T.S.
  • Organizer: The 38th Annual Meeting of the Molecular Biology Society of Japan（招待講演）
  • Place of Presentation: 神戸国際会議場（神戸）
  • Year and Date: 2015-12-03
  • Int'l Joint Research / Invited
• [Presentation] 卵子における染色体数異常の内在的な原因と老化依存的な原因 (2015)
  • Author(s): 北島智也
  • Organizer: 神戸大学重点研究チーム「資源動物のシグナル伝達制御に関する研究」ワークショップ「生殖細胞研究の最先端技術」（招待講演）
  • Place of Presentation: 神戸大学農学研究科（神戸）
  • Year and Date: 2015-09-11
  • Invited
• [Presentation] Intrinsic and age-related sources of chromosome segregation errors in mouse oocytes (2015)
  • Author(s): Kitajima, T.S.
  • Organizer: Lecture at the Institute of Animal Physiology and Genetics AS CR (招待講演)
  • Place of Presentation: Libechov (Czech Republic)
  • Year and Date: 2015-09-04
  • Int'l Joint Research / Invited
• [Presentation] Intrinsic and age-related sources of chromosome segregation errors during meiosis I in oocytes (2015)
  • Author(s): Kitajima, T.S.
  • Organizer: EMBO Workshop Dynamic Kinetochore(招待講演)
  • Place of Presentation: Copenhagen (Denmark)
  • Year and Date: 2015-05-21
  • Int'l Joint Research / Invited
• [Presentation] Intrinsic and age-related sources of chromosome segregation errors during meiosis I in oocytes (2015)
  • Author(s): Kitajima, T.S.
  • Organizer: EMBO Workshop - Dynamic kinetochore
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2015-05-18 – 2015-05-21
  • Invited
• [Presentation] Major causes of age-related chromosome segregation errors at meiosis I in oocytes. (2014)
  • Author(s): Kitajima, T.S.
  • Organizer: International Microscopy Congress (IMC)
  • Place of Presentation: Prague Congress Centre, Prague, Czech Republic
  • Year and Date: 2014-09-08
  • Invited
• [Presentation] Major causes of age-related chromosome segregation errors at meiosis I in oocytes. (2014)
  • Author(s): Kitajima, T.S.
  • Organizer: World Congress of Reproductive Biology 2014
  • Place of Presentation: Edinburgh International Conference Centre, Edinburgh, UK
  • Year and Date: 2014-09-04
  • Invited
• [Presentation] Causes of age-related chromosome segregation errors in oocytes (2014)
  • Author(s): Kitajima, T, S
  • Organizer: 第16回京都大学生命科学研究科シンポジウム
  • Place of Presentation: 京都大学芝蘭会館 稲盛ホール、京都
  • Year and Date: 2014-07-01
  • Invited
• [Book] Oogenesis: Methods and Protocols. (2016)
  • Author(s): Yoshida S., Sakakibara Y., and Kitajima T.S.
  • Total Pages: 11
  • Publisher: Springer New York
• [Remarks] 理化学研究所 染色体分配研究チーム
  • URL: http://www.cdb.riken.jp/lcs/index.html
• [Remarks] 染色体分配研究チーム 理化学研究所
  • URL: http://www.riken.jp/research/labs/cdb/chromo_segr/
• [Remarks] 染色体分配研究チーム 理化学研究所多細胞システム形成研究センター
  • URL: http://www.cdb.riken.jp/research/laboratory/kitajima.html
• [Remarks] 卵母細胞の分裂では微小管と染色体の接続が不安定
  • URL: http://www.riken.jp/pr/press/2015/20150529_1/
• [Remarks] 卵子の細胞分裂に見られる不安定性
  • URL: http://www.cdb.riken.jp/news/2015/researches/0609_8071.html
• [Remarks] 研究室Webページ
  • URL: http://www.cdb.riken.jp/lcs/index.html