Oocyte-specific spindle scaling
Project/Area Number |
26650072
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Cell biology
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Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Kitajima Tomoya 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, チームリーダー (00376641)
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Co-Investigator(Renkei-kenkyūsha) |
Kyogoku Hirohisa 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, 訪問研究員 (20726038)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 卵母細胞 / 紡錘体 / 染色体分配 / 減数分裂 |
Outline of Final Research Achievements |
This study aimed to reveal the mechanisms and roles of spindle scaling in oocytes. Four-dimensional live analysis of mouse oocytes following artificial cytoplasmic increase or decrease demonstrated that spindle volume is influenced by cytoplasmic volume. Further investigation of the functionality of the spindles indicated that a larger cytoplasm lowers the spindle functionality and increases the frequency of chromosome segregation errors. These results suggested that the large cytoplasm of oocytes is a cell-intrinsic feature that predisposes to chromosome segregation errors (Kyogoku & Kitajima 2017 Dev Cell).
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Super-resolution mapping of neuronal circuitry with an index optimized clearing agent.2016
Author(s)
Ke, M.-T., Nakai, Y., Fujimoto, S., Takayama, R., Yoshida, S., Kitajima, T.S., Sato, M., and Imai, T.
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Journal Title
Cell Reports
Volume: 14
Pages: 2718-2732
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Meikin is a conserved regulator of meiosis-I-specific kinetochore function.2015
Author(s)
Kim J, Ishiguro K, Nambu A, Akiyoshi B, Yokobayashi S, Kagami A, Ishiguro T, Pendas AM, Takeda N, Sakakibara Y, Kitajima TS, Tanno Y, Sakuno T, Watanabe Y.
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Journal Title
Nature
Volume: 517
Pages: 466-471
DOI
Related Report
Peer Reviewed
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[Presentation] 細胞動態のイメージングと定量2016
Author(s)
北島智也
Organizer
第39回日本分子生物学会年会
Place of Presentation
パシフィコ横浜(神奈川県)
Year and Date
2016-12-01 – 2016-12-01
Related Report
Invited
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[Presentation] 卵子の染色体数異常の原因2016
Author(s)
北島智也
Organizer
第39回日本分子生物学会年会
Place of Presentation
パシフィコ横浜(神奈川県)
Year and Date
2016-11-30 – 2016-11-30
Related Report
Invited
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[Presentation] 卵子の染色体数異常の原因2016
Author(s)
北島智也
Organizer
第61回日本生殖医学会学術講演会
Place of Presentation
パシフィコ横浜(神奈川県)
Year and Date
2016-11-03 – 2016-11-03
Related Report
Invited
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[Presentation] 卵子の染色体数異常の原因2016
Author(s)
北島智也
Organizer
第38回中部生殖医学会学術集会
Place of Presentation
アスト津(三重県)
Year and Date
2016-06-18 – 2016-06-18
Related Report
Invited
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[Presentation] 卵子の染色体数異常の原因2016
Author(s)
北島智也
Organizer
ナースのためのART医学セミナー2016年
Place of Presentation
六本木アカデミーヒルズ49(東京都)
Year and Date
2016-05-22 – 2016-05-22
Related Report
Invited
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