| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
To produce transgenic animals using ES cells, ES cells must be capable of germline transmission. We previously established novel germline-competent chicken ES cell lines using chicken leukemia inhibitory factor (chLIF); however, they possessed very little capacity to differentiate into germline cells. In this study, we have selected the Japanese quail as a model animal by the advantages of short generation time. Using quail ES (qES) cell lines derived from various strains, we examined the optimal culture conditions and analyzed the effect of gene modification on chromosomal abnormalities. We demonstrate that qES-like (qESL) cells cultured have the capacity for long-term successive subculture and the competency for chimeric formation after injection into recipient embryos. To confirm the signaling pathways required for maintenance of self-renewal and survival of qESL cells, they were cultured in the presence of JAK inhibitor.
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