Project/Area Number |
26650086
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Developmental biology
|
Research Institution | Kwansei Gakuin University |
Principal Investigator |
|
Co-Investigator(Renkei-kenkyūsha) |
KIM Hon-Song 関西学院大学, 理工学研究科, 博士研究員 (70469899)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | ADAMTSプロテアーゼ / リボソーム / 細胞移動 / 線虫 / 器官形成 / 細胞外マトリックス |
Outline of Final Research Achievements |
MIG-17, an ADAMTS metalloprotease controls migration of gonadal distal tip cells (DTCs) through regulation of extracellular matrix in C. elegans. In the present study, we asked why the mutation in a ribosomal protein RPL-20(G82R) can suppress the DTC migration defect in the mig-17 mutants. Although we could not reach a clear understanding of the mechanism, we found that RPL-20(G82R) does not affect the regulatory mechanism of translational initiation. We also showed that RPL-20(G82R) is not secreted, but that it appears to act through unknown secreted proteins to suppress the mig-17 defects. We found that the RPL-20 activity is especially important in the intestine for the suppression to occur.
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