Role of a ribosomal protein in the ADAMTS protease pathway regulating cell migration

• Project/Area Number: 26650086
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kwansei Gakuin University
• Principal Investigator: Nishiwaki Kiyoji 関西学院大学, 理工学部, 教授 (30342827)
• Co-Investigator(Renkei-kenkyūsha): KIM Hon-Song 関西学院大学, 理工学研究科, 博士研究員 (70469899)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ADAMTSプロテアーゼ / リボソーム / 細胞移動 / 線虫 / 器官形成 / 細胞外マトリックス

Outline of Final Research Achievements

MIG-17, an ADAMTS metalloprotease controls migration of gonadal distal tip cells (DTCs) through regulation of extracellular matrix in C. elegans. In the present study, we asked why the mutation in a ribosomal protein RPL-20(G82R) can suppress the DTC migration defect in the mig-17 mutants. Although we could not reach a clear understanding of the mechanism, we found that RPL-20(G82R) does not affect the regulatory mechanism of translational initiation. We also showed that RPL-20(G82R) is not secreted, but that it appears to act through unknown secreted proteins to suppress the mig-17 defects. We found that the RPL-20 activity is especially important in the intestine for the suppression to occur.

Research Products

• [Journal Article] Organ Length Control by an ADAMTS Extracellular Protease in Caenorhabditis elegans (2016)
  • Author(s): Shibata, Y., Kawakado,Y., Hori,N., Tanaka, K., Inoue, R., Takano, T., Kubota, Y., Nishiwaki,K.
  • Journal Title: G3 Volume: 6 Issue: 5 Pages: 1449-1457
  • DOI: 10.1534/g3.116.028019
  • Peer Reviewed
• [Journal Article] Mg2+ Extrusion from Intestinal Epithelia by CNNM Proteins Is Essential for Gonadogenesis via AMPK-TORC1 Signaling in Caenorhabditis elegans. (2016)
  • Author(s): Ishii T, Funato Y, Hashizume O, Yamazaki D, Hirata Y, Nishiwaki K, Kono N, Arai H, Miki H.
  • Journal Title: PLoS Genet. Volume: 12 Issue: 8 Pages: e1006276-e1006276
  • DOI: 10.1371/journal.pgen.1006276
  • Peer Reviewed / Open Access
• [Journal Article] Control of the basement membrane and cell migration by ADAMTS proteinases: Lessons from C. elegans genetics. (2015)
  • Author(s): Kim, H.-S.and Nishiwaki, K.
  • Journal Title: Matrix Biology Volume: 44-46C Pages: 64-69
  • DOI: 10.1016/j.matbio.2015.01.001
  • Peer Reviewed / Open Access
• [Journal Article] The BED finger domain protein MIG-39 halts migration of distal tip cells in Caenorhabditis elegans. (2015)
  • Author(s): Kikuchi, T., Shibata, Y., Kim, H.-S., Kubota, Y., Yoshina, S., Mitani, S. and Nishiwaki, K.
  • Journal Title: Developmental Biology Volume: 397 Pages: 151-161
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] BLMP-1/Blimp-1 Regulates the Spatiotemporal Cell Migration Pattern in C. elegans. (2014)
  • Author(s): Huang, T-F., Cho, C-Y., Cheng, Y-T., Huang, J-W., Wu, Y-Z., Yeh, A. Y-C., Nishiwaki, K., Chang, S-C., and Wu, Y-C
  • Journal Title: PLoS Genetics Volume: 10
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] HTZ-1/H2A.z and MYS-1/MYST HAT act redundantly to maintain cell fates in somatic gonadal cells through repression of ceh-22 in Caenorhabditis elegans. (2014)
  • Author(s): Shibata Y, Sawa H, Nishiwaki K
  • Journal Title: Development Volume: 141(1) Issue: 1 Pages: 209-218
  • DOI: 10.1242/dev.090746
  • Peer Reviewed
• [Presentation] 線虫の咽頭サイズを制御するgob-1遺伝子の解析 (2016)
  • Author(s): 井上領、松井那美香、井関晶太、 柴田幸政、西脇清二
  • Organizer: 第３９回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] 線虫C. elegansにおけるROSを介した咽頭サイズ制御機構の解析 (2016)
  • Author(s): 足立将大、坂東里美、平本和也、加嶋紀彦、森部弘樹、柴田幸政、西脇清二
  • Organizer: 第３９回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] 線虫のADAMTSプロテアーゼによる基底膜IV型コラーゲン制御の解析 (2016)
  • Author(s): 中村久子、高野智美、柴田幸政、西脇清二
  • Organizer: 第３９回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] Regulation of pharynx size by apical and basal extracellular matrices in C. elegans. (2016)
  • Author(s): Shibata, Y., Sakata, S., Kawakado, Y., Hori, N., Tanaka, K., Inoue, R., Takano, T., Kubota, Y., Nishiwaki, K.
  • Organizer: CDB Symposium 2016
  • Place of Presentation: 理化学研究所（兵庫県神戸市）
  • Year and Date: 2016-03-28
  • Int'l Joint Research
• [Presentation] Cell-fate-determination mechanism that functions redundantly with BET family protein. (2015)
  • Author(s): Shibata, Y., Konakawa, R., Sawa, H., Nishiwaki, K.
  • Organizer: 第３８回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] Involvement of a ribosomal protein in the MIG-17/ADAMTS-dependent regulation of cell migration in C. elegans (2015)
  • Author(s): Kondo, S., Yamaoka, R., Kim, H.-S., Nishiwaki, K.
  • Organizer: 第３８回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] pqn-74 regulates pharynx size in C. elegans. (2015)
  • Author(s): Sakata, S., Tanaka, K., Iseki, M., Nishiwaki, K.
  • Organizer: 第３８回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] The TFIIH and SWI/SNF complexes are required for the cell fate maintenance in C. elegans. (2015)
  • Author(s): Shibata, Y., Konakawa, R., Sawa, H., Nishiwaki, K.
  • Organizer: 20th International C. elegans Meeting
  • Place of Presentation: Los Angeles (USA)
  • Year and Date: 2015-06-24
  • Int'l Joint Research
• [Presentation] The TFIIH complex and the SWI/SNF complex is required for the cell fate maintenance in C. elegans (2014)
  • Author(s): Shibata, Y., Takeshita, H., Konakawa, R., Sawa, H., Nishiwaki, K.
  • Organizer: 第３７回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Nuclear membrane proteins act in transport of the Netrin receptor UNC-5 in cell migration in C. elegans. (2014)
  • Author(s): Kim, H.-S., Nishiwaki, K.
  • Organizer: 第３７回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Analysis of flp-10 that controls migration of the gonadal leader cells in C. elegans (2014)
  • Author(s): Morita, K., Nakaya, K., Kim, H.-S., Nishiwaki, K.
  • Organizer: 第３７回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Genetic interactions among ADAMTS metalloproteases and basement membrane molecules in cell migration in C. elegans (2014)
  • Author(s): Imanishi, A., Takano, T., Nishiwaki, K.
  • Organizer: 第３７回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] pqn-74 regulates pharynx size in C. elegans (2014)
  • Author(s): Sakata, S., Tanaka, K., Iseki, M., Shibata, Y., Nishiwaki, K.
  • Organizer: 第３７回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Involvement of a ribosomal protein in the MIG-17/ADAMTS-dependent regulation of cell migration in C. elegans (2014)
  • Author(s): Kondo, S., Yamaoka, R., Kim, H.-S., Nishiwaki, K.
  • Organizer: 第３７回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] HTZ-1 and MYS-1 act redundantly to maintain cell fates in somatic gonadal cells through repression of ceh-22 in Caenorhabditis elegans (2014)
  • Author(s): Shibata, Y., Sawa, H., Nishiwaki, K.
  • Organizer: The 6th Asia-Pacific C. elegans Meeting
  • Place of Presentation: 奈良県立新公会堂（奈良県奈良市）
  • Year and Date: 2014-07-15 – 2014-07-19
• [Presentation] Nuclear membrane proteins act in transport of the Netrin receptor UNC-5 in cell migration in C. elegans. (2014)
  • Author(s): Kim, H.-S., Nishiwaki, K.
  • Organizer: The 6th Asia-Pacific C. elegans Meeting
  • Place of Presentation: 奈良県立新公会堂（奈良県奈良市）
  • Year and Date: 2014-07-15 – 2014-07-19
• [Presentation] Analysis of flp-10 that controls migration of the gonadal leader cells in C. elegans (2014)
  • Author(s): Morita, K., Kim, H.-S., Nishiwaki, K
  • Organizer: The 6th Asia-Pacific C. elegans Meeting
  • Place of Presentation: 奈良県立新公会堂（奈良県奈良市）
  • Year and Date: 2014-07-15 – 2014-07-19
• [Presentation] Genetic interactions among ADAMTS metalloproteases and basement membrane molecules in cell migration in C. elegans (2014)
  • Author(s): Imanishi, A., Takano, T., Nishiwaki, K.
  • Organizer: The 6th Asia-Pacific C. elegans Meeting
  • Place of Presentation: 奈良県立新公会堂（奈良県奈良市）
  • Year and Date: 2014-07-15 – 2014-07-19
• [Remarks] 西脇研究室ＨＰ
  • URL: http://sci-tech.ksc.kwansei.ac.jp/~nishiwaki/
• [Remarks] 西脇研究室ホームページ
  • URL: http://sci-tech.ksc.kwansei.ac.jp/~nishiwaki/