Development of a new method for the quantification of isoprenoid intermediates and analysis of the localization of the isoprenoid metabolic pathways in chloroplasts
| Project/Area Number |
26650088
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Hokkaido University |
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Principal Investigator |
TANAKA Ryouichi 北海道大学, 低温科学研究所, 准教授 (20311516)
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| Project Period (FY) |
2014-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 葉緑体 |
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| Outline of Final Research Achievements |
We aimed at the development of a method to quantify geranylgeranyl-diphosphate (GGDP) and phytyl-diphosphate (PDP) in this study. First, we have tested whether geranylgeranyl transferase can conjugate GGDP and PDP to a dansylated peptide, which can be easily detected by a liquid chromatography system according to the method of Tong et al. 2013. However, this method only detect GGDP-conjugated peptide. Then, we attempted to use chlorophyll synthase activity to detect GGDP- or PDP-conjugate to chlorophyllide. However, we failed to overexpress this enzyme by an E. coli system, cultured insect cells (Sf9), and the MembraneMax cell-free expression system.
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Report
(2 results)
Research Products
(2 results)
