• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of genetic factors involved in interaction between rhizobium and host plant through type III secretion system

Research Project

Project/Area Number 26650089
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionTohoku University

Principal Investigator

Sato Shusei  東北大学, 生命科学研究科, 准教授 (70370921)

Co-Investigator(Kenkyū-buntansha) KANEKO TAKAKAZU  京都産業大学, 総合生命科学部, 教授 (80370922)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords根粒菌-宿主相互作用 / TypeIII分泌系 / エフェクタータンパク質 / ミヤコグサ / 組換え近交系統 / ナチュラルバリエーション / 根粒菌ー宿主相互作用
Outline of Final Research Achievements

In an attempt to accumulate the information on the interaction between rhizobium and legume plants medicated by type III secretion system (T3SS) effectors, we conducted molecular genetic analyses using a model legume Lotus japonicus and Bradyrhizobium elkanii. As the results of infection analysis and Tn5 mutant screening, we identified that two types of immune responses, block of infection and prevention of nodule maturation, induced by different effectors. Based on the QTL analysis using RILs and GWAS using wild accessions of L. japonicus, we detected multiple candidate loci related to the responses caused by T3SS effectors.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (6 results) (of which Invited: 1 results) Book (2 results)

  • [Int'l Joint Research] Aarhus University(Denmark)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Soybean Plants Carrying the Rj4 Allele2015

    • Author(s)
      Faruque OM., Miwa H., Yasuda M., Fujii Y., Kaneko T., Sato S. and Okazaki S.
    • Journal Title

      Applied Environental Microbiology

      Volume: 81 Issue: 19 Pages: 6710-6717

    • DOI

      10.1128/aem.01942-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Lotus japonicus cytokinin receptors work partially redundantly to mediate nodule formation2014

    • Author(s)
      Held M, Hou H, Miri M, Huynh C, Ross L, Hossain MS, Sato S, Tabata S, Perry J, Wang TL, Szczyglowski K.
    • Journal Title

      Plant Cell

      Volume: 26 Issue: 2 Pages: 678-694

    • DOI

      10.1105/tpc.113.119362

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Re-sequencing of recombinant inbred lines of Lotus japonicus toward upgrading of genome information2016

    • Author(s)
      Shusei Sato, Niraj Shah, Mikkel Heide Schierup, Shohei Kusakabe, Hideki Hirakawa, Jens Stougaard, Stig U. Andersen
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学上田キャンパス(岩手県盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Analysis of type III effector proteins of Bradyrhizobium elkanii USDA61 which cause effector triggered immunity response in Lotus japonicus.2016

    • Author(s)
      Shohei Kusakabe, Takakazu Kaneko, Michiko Yasuda, Hiroki Miwa, Shin Okazaki, Shusei Sato
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学上田キャンパス(岩手県盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] ミヤコグサとBradyrhizobium elkanii USDA61株とのⅢ型分泌系を介した相互作用に関与する因子の解析2015

    • Author(s)
      日下部 翔平、金子 貴一、安田 美智子、三輪 大樹、岡崎 伸、佐藤 修正
    • Organizer
      植物微生物研究会 第25回研究交流会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] Bradyrhizobium elkanii USDA61株のエフェクターに対する反応のミヤコグサ系統間差の解析2015

    • Author(s)
      日下部 翔平、金子 貴一、安田 美智子、三輪 大樹、岡崎 伸、佐藤 修正
    • Organizer
      第56回 日本植物生理学会
    • Place of Presentation
      東京農業大学(東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Establishment of the Lotus japonicus genomic information toward functional genomics.2014

    • Author(s)
      Shusei Sato
    • Organizer
      3rd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Place of Presentation
      New International Convention & Exposition Center (Chengdu, China)
    • Year and Date
      2014-10-28 – 2014-10-30
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Bradyrhizobium elkanii USDA61株接種に対するミヤコグサ系統間差の解析2014

    • Author(s)
      日下部 翔平、金子 貴一、安田 美智子、三輪 大樹、岡崎 伸、佐藤 修正
    • Organizer
      植物微生物研究会 第24回研究交流会
    • Place of Presentation
      佐賀大学(佐賀県佐賀市)
    • Year and Date
      2014-09-19 – 2014-09-21
    • Related Report
      2014 Research-status Report
  • [Book] The Lotus japonicus genome (Chapter 4. Genome sequencing)2014

    • Author(s)
      Shusei Sato and Stig Andersen
    • Total Pages
      6
    • Publisher
      Springer
    • Related Report
      2014 Research-status Report
  • [Book] The Lotus japonicus genome (Chapter 3. Genetic linkage maps, synteny and map-based cloning)2014

    • Author(s)
      Niels Sandal and Shusei Sato
    • Total Pages
      11
    • Publisher
      Springer
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi