Analysis of genetic factors involved in interaction between rhizobium and host plant through type III secretion system

• Project/Area Number: 26650089
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tohoku University
• Principal Investigator: Sato Shusei 東北大学, 生命科学研究科, 准教授 (70370921)
• Co-Investigator(Kenkyū-buntansha): KANEKO TAKAKAZU 京都産業大学, 総合生命科学部, 教授 (80370922)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 根粒菌-宿主相互作用 / TypeIII分泌系 / エフェクタータンパク質 / ミヤコグサ / 組換え近交系統 / ナチュラルバリエーション / 根粒菌ー宿主相互作用

Outline of Final Research Achievements

In an attempt to accumulate the information on the interaction between rhizobium and legume plants medicated by type III secretion system (T3SS) effectors, we conducted molecular genetic analyses using a model legume Lotus japonicus and Bradyrhizobium elkanii. As the results of infection analysis and Tn5 mutant screening, we identified that two types of immune responses, block of infection and prevention of nodule maturation, induced by different effectors. Based on the QTL analysis using RILs and GWAS using wild accessions of L. japonicus, we detected multiple candidate loci related to the responses caused by T3SS effectors.

Research Products

• [Int'l Joint Research] Aarhus University(Denmark)
• [Journal Article] Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Soybean Plants Carrying the Rj4 Allele (2015)
  • Author(s): Faruque OM., Miwa H., Yasuda M., Fujii Y., Kaneko T., Sato S. and Okazaki S.
  • Journal Title: Applied Environental Microbiology Volume: 81 Issue: 19 Pages: 6710-6717
  • DOI: 10.1128/aem.01942-15
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lotus japonicus cytokinin receptors work partially redundantly to mediate nodule formation (2014)
  • Author(s): Held M, Hou H, Miri M, Huynh C, Ross L, Hossain MS, Sato S, Tabata S, Perry J, Wang TL, Szczyglowski K.
  • Journal Title: Plant Cell Volume: 26 Issue: 2 Pages: 678-694
  • DOI: 10.1105/tpc.113.119362
  • Peer Reviewed / Open Access
• [Presentation] Re-sequencing of recombinant inbred lines of Lotus japonicus toward upgrading of genome information (2016)
  • Author(s): Shusei Sato, Niraj Shah, Mikkel Heide Schierup, Shohei Kusakabe, Hideki Hirakawa, Jens Stougaard, Stig U. Andersen
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学上田キャンパス（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] Analysis of type III effector proteins of Bradyrhizobium elkanii USDA61 which cause effector triggered immunity response in Lotus japonicus. (2016)
  • Author(s): Shohei Kusakabe, Takakazu Kaneko, Michiko Yasuda, Hiroki Miwa, Shin Okazaki, Shusei Sato
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学上田キャンパス（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] ミヤコグサとBradyrhizobium elkanii USDA61株とのⅢ型分泌系を介した相互作用に関与する因子の解析 (2015)
  • Author(s): 日下部 翔平、金子 貴一、安田 美智子、三輪 大樹、岡崎 伸、佐藤 修正
  • Organizer: 植物微生物研究会 第25回研究交流会
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2015-09-14
• [Presentation] Bradyrhizobium elkanii USDA61株のエフェクターに対する反応のミヤコグサ系統間差の解析 (2015)
  • Author(s): 日下部 翔平、金子 貴一、安田 美智子、三輪 大樹、岡崎 伸、佐藤 修正
  • Organizer: 第56回 日本植物生理学会
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Establishment of the Lotus japonicus genomic information toward functional genomics. (2014)
  • Author(s): Shusei Sato
  • Organizer: 3rd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: New International Convention & Exposition Center (Chengdu, China)
  • Year and Date: 2014-10-28 – 2014-10-30
  • Invited
• [Presentation] Bradyrhizobium elkanii USDA61株接種に対するミヤコグサ系統間差の解析 (2014)
  • Author(s): 日下部 翔平、金子 貴一、安田 美智子、三輪 大樹、岡崎 伸、佐藤 修正
  • Organizer: 植物微生物研究会 第24回研究交流会
  • Place of Presentation: 佐賀大学（佐賀県佐賀市）
  • Year and Date: 2014-09-19 – 2014-09-21
• [Book] The Lotus japonicus genome (Chapter 4. Genome sequencing) (2014)
  • Author(s): Shusei Sato and Stig Andersen
  • Total Pages: 6
  • Publisher: Springer
• [Book] The Lotus japonicus genome (Chapter 3. Genetic linkage maps, synteny and map-based cloning) (2014)
  • Author(s): Niels Sandal and Shusei Sato
  • Total Pages: 11
  • Publisher: Springer