• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of plant RNA granules with electron microscopy

Research Project

Project/Area Number 26650090
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionThe University of Tokyo

Principal Investigator

HAMADA Takahiro  東京大学, 総合文化研究科, 助教 (20452534)

Co-Investigator(Renkei-kenkyūsha) WATANABE Yuichiro  東京大学, 大学院総合文化研究科, 教授 (60183125)
TOYOOKA Kiminori  理化学研究所, 環境資源科学研究センター, 上級研究員 (10360596)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsRNA顆粒 / ストレス顆粒 / P-body / シロイヌナズナ / 植物 / 免疫電顕
Outline of Final Research Achievements

RNA granules, which composed of various RNAs and proteins, are cytoplasmic granules to participate in post-transcriptional regulations including mRNA transport, translation repression, RNA degradation, small RNA functions and so on. We focused on representative RNA granules, stress granules and P-bodies, and investigated the morphologies of RNA granules by electron microscopy. Our analyses revealed that RNA granules are about from 100 to 300 nm in diameter and have a filamentous pattern in granules. In addition, we also found that ribosomes are not observed in RNA granules. Our research figured out the morphology of RNA granules in plant cells and provided a base for future plant RNA granules researches.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Invited: 3 results)

  • [Journal Article] Lessons from in vitro reconstitution analyses of plant microtubule-associated proteins.2014

    • Author(s)
      Takahiro Hamada
    • Journal Title

      Front Plant Sci.

      Volume: 5 Pages: 409-409

    • DOI

      10.3389/fpls.2014.00409

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Microtubule organization and microtubule-associated proteins in plant cells.2014

    • Author(s)
      Takahiro Hamada
    • Journal Title

      Int Rev Cell Mol Biol.

      Volume: 312 Pages: 1-52

    • DOI

      10.1016/b978-0-12-800178-3.00001-4

    • ISBN
      9780128001783
    • Related Report
      2014 Research-status Report
  • [Presentation] シロイヌナズナにおけるストレス顆粒形成機構の解析2016

    • Author(s)
      矢光真子、福澤麻里奈、佐藤繭子、亀井保博、柳川由紀、西村いくこ、豊岡公徳、渡邊雄一郎、濱田隆宏
    • Organizer
      第5回植物RNAワークショップ
    • Place of Presentation
      東京大学(東京都目黒区)
    • Year and Date
      2016-01-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] 植物におけるRNA顆粒ダイナミクス2015

    • Author(s)
      濱田隆宏、渡邊雄一郎
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Relationships between components of cytoplasmic RNA granules and small RNA metabolism2015

    • Author(s)
      Takahiro Hamada
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      新潟朱鷺メッセ(新潟県新潟市)
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] シロイヌナズナにおけるストレス顆粒形成機構の解析2015

    • Author(s)
      福澤麻里奈、佐藤繭子、元村一基、柳川由紀、西村いくこ、豊岡公徳、渡邊雄一郎、濱田隆宏
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京都・世田谷)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 植物のRNA顆粒を介した温度変化応答2015

    • Author(s)
      濱田隆宏
    • Organizer
      第9回バイオイメージングフォーラム
    • Place of Presentation
      基礎生物学研究所(愛知県・岡崎)
    • Year and Date
      2015-01-26 – 2015-01-27
    • Related Report
      2014 Research-status Report
    • Invited

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi