| Project/Area Number |
26650092
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Ozeki Yoshihiro 東京農工大学, 工学(系)研究科(研究院), 教授 (50185592)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | クサギ / トリコトミン / インドールアルカロイド / N-グルコシルトランスフェラーゼ |
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| Outline of Final Research Achievements |
Trichotomine produced as a deep blue pigment in Clerodendron trichotomum. The structure was known as a bis(indole) alkaloid and biosynthesized from tryptophan, however the biosynthetic pathway of trichotomine has not been confirmed. This study intended to be clarified the biosynthetic pathway and identified their enzymatic genes. The transcriptional gene sequencing data of next generation sequence was constructed from total RNA that extracted from C. trichotomum fruit. Seventeen genes of the enzymes in tryptophan metabolic pathway were extracted and also extracted six candidate genes of the N-glucosyltransferase that might be involved in glucosylation of trichotomine. The defect of trichotomine biosynthetic mutant callus was isolated from the trichotomine spontaneous biosynthesis calli. In future, the expression analysis of the extracted genes in this study is going to compare from normal trichotomine calli and mutant callus reveal the reason of the mutant is occurred.
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