Isolation of novel-type photosynthesis mutants of Chlamydomonas by phototaxis-based screening

• Project/Area Number: 26650093
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Wakabayashi Ken-ichi 東京工業大学, 科学技術創成研究院, 准教授 (80420248)
• Co-Investigator(Kenkyū-buntansha): 島袋 勝弥 宇部工業高等専門学校, 物質工学科, 准教授 (70618446)
• Research Collaborator: Hisabori Toru 東京工業大学, 科学技術創成研究院, 教授 ; Ueki Noriko 東京工業大学, 科学技術創成研究院, 博士研究員 ; Ide Takahiro 東京工業大学, 資源化学研究所, 博士研究員
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 走光性 / 光合成 / レドックス / 鞭毛 / クラミドモナス

Outline of Final Research Achievements

For phototrophic microorganisms, it is important to inhabit under suitable light environment for phototaxis. Previously, we found that Chlamydomonas reinhardtii, a model organism to study photo-behavioral responses, shows positive phototaxis when cell is oxidized, and negative phototaxis when it is reduced. We came up with the idea to use this phenomenon as a new criterion for screening of novel-type photosynthesis mutants. In this research, we isolated three types of phototaxis-sign mutants, and one of them shows significantly high photosynthetic activity.

Research Products

• [Journal Article] 緑藻クラミドモナスの鞭毛運動と光行動 (2017)
  • Author(s): 若林憲一、中島昌子、井手隆広、植木紀子
  • Journal Title: 日本プランクトン学会報 Volume: 64 Pages: 61-66
  • NAID: 130007609952
  • Peer Reviewed
• [Journal Article] A New Morphological Type of Volvox from Japanese Large Lakes and Recent Divergence of this Type and V. ferrisii in Two Different Freshwater Habitats. (2016)
  • Author(s): Nozaki H, Ueki N, Isaka N, Saigo T, Yamamoto K, Matsuzaki R, Takahashi F, Wakabayashi K, Kawachi M.
  • Journal Title: PLoS One Volume: 11 Issue: 11 Pages: e0167148-e0167148
  • DOI: 10.1371/journal.pone.0167148
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of the agg1 mutation responsible for negative phototaxis in a "wild-type" strain of Chlamydomonas reinhardtii (2016)
  • Author(s): Ide, T., Mochiji, S., Ueki, N., Yamaguchi, K., Shigenobu, S., Hirono, M., Wakabayashi, K.
  • Journal Title: Biochemistry and Biophysics Reports Volume: 7 Pages: 379-385
  • DOI: 10.1016/j.bbrep.2016.07.016
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii (2016)
  • Author(s): Ueki, N., Ide, T., Mochiji, S., Kobayashi, Y., Tokutsu, R., Ohnishi, N., Yamaguchi, K., Shigenobu, S., Tanaka, K., Minagawa, J., Hisabori, T., Hirono, M., Wakabayashi, K.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 印刷中 Issue: 19 Pages: 5266-5304
  • DOI: 10.1073/pnas.1525538113
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cooperative binding of the outer arm docking complex underlies the regular arrangement of outer arm dynein in the axoneme. (2014)
  • Author(s): Mikito Owa, Akane Furuta, Jiro Usukura, Fumio Arisaka, Stephen M. King, George B. Witman, Ritsu Kamiya, Ken-ichi Wakabayashi
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 111(26) Issue: 26 Pages: 9455-9460
  • DOI: 10.1073/pnas.1403101111
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 緑藻の光行動における鞭毛運動調節：クラミドモナスとボルボックス (2017)
  • Author(s): 植木紀子、若林憲一
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島・鹿児島大学群元キャンパスンパス
  • Year and Date: 2017-03-16
• [Presentation] クラミドモナスのγ-tubulin 優性突然変異 bld13 (2017)
  • Author(s): 中澤友紀 、堀井麻央 、渡辺早紀 、大月萌子 、苗加彰 、若林憲一 、廣野雅文
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 神戸・神戸国際会議場
  • Year and Date: 2017-01-06
• [Presentation] クラミドモナスにおける走光性の正負と「利き鞭毛」の関係 (2017)
  • Author(s): 中島昌子 、植木紀子 、若林憲一
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 神戸・神戸国際会議場
  • Year and Date: 2017-01-06
• [Presentation] ボルボックスのカルシウム依存的繊毛打逆転と走光性 (2017)
  • Author(s): 植木紀子、若林憲一
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 神戸・神戸国際会議場
  • Year and Date: 2017-01-06
• [Presentation] Photo-behavior in green algae: how light modulates flagellar motility (2017)
  • Author(s): Masako Nakajima, Noriko Ueki, Ken-ichi Wakabayashi
  • Organizer: 光合成科学：エネルギーとバイオマス
  • Place of Presentation: 横浜・東工大すずかけホール
  • Int'l Joint Research / Invited
• [Presentation] Modulation of flagellar motility during phototactic turning in Chlamydomonas (2016)
  • Author(s): Masako Nakajima, Noriko Ueki, Ken-ichi Wakabayashi
  • Organizer: The 28th CDB Meeting: Cilia and Centrosomes: Current Advances and Future Directions
  • Place of Presentation: 神戸・理研CDB
  • Year and Date: 2016-11-27
  • Int'l Joint Research
• [Presentation] The molecular toolbox for building axonemal microtubules (2016)
  • Author(s): Mikito Owa, Takayuki Uchihashi, Haruaki Yanagisawa, Takashi Yamano, Hiro Iguchi, Hideya Fukuzawa, Ken-ichi Wakabayashi, Toshio Ando, Masahide Kikkawa
  • Organizer: The 28th CDB Meeting: Cilia and Centrosomes: Current Advances and Future Directions
  • Place of Presentation: 神戸・理研CDB
  • Year and Date: 2016-11-27
  • Int'l Joint Research
• [Presentation] 緑藻クラミドモナスの走光性発現メカニズム (2016)
  • Author(s): 若林憲一
  • Organizer: 遺伝研研究会「水圏微生物のエネルギー利用戦略とその相互作用に関する研究会」
  • Place of Presentation: 三島・遺伝研
  • Year and Date: 2016-11-03
  • Invited
• [Presentation] Visualization and quantification of the redox potential in the Chlamydomonas flagella (2016)
  • Author(s): Yuta Nishimaki, Kazunori Sugiura, Toru Hisabori, Ken-ichi Wakabayashi
  • Organizer: The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: 京都・京都国際会館
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii (2016)
  • Author(s): Noriko Ueki, Takahiro Ide, Shota Mochiji, Yuki Kobayashi, Ryutaro Tokutsu, Norikazu Ohnishi, Katushi Yamaguchi, Shuji Shigenobu, Kan Tanaka, Jun Minagawa, Toru Hisabori, Masafumi Hirono, Ken-ichi Wakabayashi
  • Organizer: The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: 京都・京都国際会館
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Isolation of a non-phototactic Chlamydomonas mutant that shows alternate flagellar beatings (2016)
  • Author(s): Akinori Koitabashi, Masako Nakajima, Noriko Ueki, Kogiku Shiba, Takashi Ymano, Hideya Fukuzawa, Inaba Kazuo, Toru Hisabori, Ken-ichi Wakabayashi
  • Organizer: The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: 京都・京都国際会館
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Identification of the agg1 mutation responsible for negative phototaxis in a "wild-type" strain of Chlamydomonas reinhardtii (2016)
  • Author(s): Takahiro Ide, Shota Mochuji, Noriko Ueki, Katsushi Yamaguchi, Shuji Shigenobu, Masafumi Hirono, Ken-ichi Wakabayashi
  • Organizer: The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: 京都・京都国際会館
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] 緑藻クラミドモナスとボルボックスの走光性のしくみ (2016)
  • Author(s): 若林憲一
  • Organizer: 第34回エアロアクアバイオメカニズム学会定例講演会
  • Place of Presentation: 東京・東京工業大学大岡山キャンパス
  • Year and Date: 2016-03-23
  • Invited
• [Presentation] 緑藻クラミドモナスの走光性メカニズム - 光受容と鞭毛運動の連関 - (2016)
  • Author(s): 若林憲一
  • Organizer: 日本プランクトン学会春季シンポジウム「光環境を巡る植物プランクトンの生理生態学的最前線」
  • Place of Presentation: 東京・東京大学本郷キャンパス
  • Year and Date: 2016-03-14
  • Invited
• [Presentation] Calcium-dependent photobehavior of the unicellular green alga Chlamydomonas reinhardtii (2015)
  • Author(s): Ken-ichi Wakabayashi
  • Organizer: 第38回日本分子生物学会年会・第８８回日本生化学会大会合同大会
  • Place of Presentation: 神戸・ポートアイランド
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] クラミドモナス走光性符号決定における眼点の役割 (2015)
  • Author(s): 植木紀子、持地翔太 、井手隆広 、山口勝司 、重信秀治 、得津隆太郎 、大 西紀和、皆川純、廣野雅文、若林憲一
  • Organizer: 日本植物生理学会第56回年会
  • Place of Presentation: 東京農業大学（東京都）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] クラミドモナス走光性符号決定における眼点の役割 (2015)
  • Author(s): 植木紀子, 持地翔太, 井手隆広, 廣野雅文, 若林憲一
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 学習院大学（東京都）
  • Year and Date: 2015-01-07 – 2015-01-09
• [Presentation] クラミドモナス走光性符号決定における眼点の役割 (2014)
  • Author(s): 若林憲一 、持地翔太 、植木紀子 、井手隆広 、山口勝司 、重信秀治 、得津隆太郎 、大 西紀和、皆川純、廣野雅文
  • Organizer: 第11回クラミドモナス研究会
  • Place of Presentation: かるぽーと（高知県）
  • Year and Date: 2014-10-03 – 2014-10-04
• [Presentation] 緑藻ボルボックス目における光反応行動調節機構 (2014)
  • Author(s): 植木紀子、箕浦高子、若林憲一
  • Organizer: 第11回クラミドモナス研究会
  • Place of Presentation: かるぽーと（高知県）
  • Year and Date: 2014-10-03 – 2014-10-04
• [Presentation] Sign-Control of Chlamydomonas Phototaxis by Redox Poise (2014)
  • Author(s): Ken-ichi Wakabayashi
  • Organizer: 16th International Congress on Photobiology
  • Place of Presentation: コルドバ国立大学
  • Year and Date: 2014-09-08 – 2014-09-12
  • Invited
• [Remarks] 久堀・若林研究室ウェブサイト
  • URL: http://www.res.titech.ac.jp/~junkan/Hisabori_HomePage/index.html