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Isolation of novel-type photosynthesis mutants of Chlamydomonas by phototaxis-based screening

Research Project

Project/Area Number 26650093
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionTokyo Institute of Technology

Principal Investigator

Wakabayashi Ken-ichi  東京工業大学, 科学技術創成研究院, 准教授 (80420248)

Co-Investigator(Kenkyū-buntansha) 島袋 勝弥  宇部工業高等専門学校, 物質工学科, 准教授 (70618446)
Research Collaborator Hisabori Toru  東京工業大学, 科学技術創成研究院, 教授
Ueki Noriko  東京工業大学, 科学技術創成研究院, 博士研究員
Ide Takahiro  東京工業大学, 資源化学研究所, 博士研究員
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords走光性 / 光合成 / レドックス / 鞭毛 / クラミドモナス
Outline of Final Research Achievements

For phototrophic microorganisms, it is important to inhabit under suitable light environment for phototaxis. Previously, we found that Chlamydomonas reinhardtii, a model organism to study photo-behavioral responses, shows positive phototaxis when cell is oxidized, and negative phototaxis when it is reduced. We came up with the idea to use this phenomenon as a new criterion for screening of novel-type photosynthesis mutants. In this research, we isolated three types of phototaxis-sign mutants, and one of them shows significantly high photosynthetic activity.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (26 results)

All 2017 2016 2015 2014 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results,  Acknowledgement Compliant: 4 results) Presentation (20 results) (of which Int'l Joint Research: 7 results,  Invited: 6 results) Remarks (1 results)

  • [Journal Article] 緑藻クラミドモナスの鞭毛運動と光行動2017

    • Author(s)
      若林憲一、中島昌子、井手隆広、植木紀子
    • Journal Title

      日本プランクトン学会報

      Volume: 64 Pages: 61-66

    • NAID

      130007609952

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A New Morphological Type of Volvox from Japanese Large Lakes and Recent Divergence of this Type and V. ferrisii in Two Different Freshwater Habitats.2016

    • Author(s)
      Nozaki H, Ueki N, Isaka N, Saigo T, Yamamoto K, Matsuzaki R, Takahashi F, Wakabayashi K, Kawachi M.
    • Journal Title

      PLoS One

      Volume: 11 Issue: 11 Pages: e0167148-e0167148

    • DOI

      10.1371/journal.pone.0167148

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of the agg1 mutation responsible for negative phototaxis in a "wild-type" strain of Chlamydomonas reinhardtii2016

    • Author(s)
      Ide, T., Mochiji, S., Ueki, N., Yamaguchi, K., Shigenobu, S., Hirono, M., Wakabayashi, K.
    • Journal Title

      Biochemistry and Biophysics Reports

      Volume: 7 Pages: 379-385

    • DOI

      10.1016/j.bbrep.2016.07.016

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii2016

    • Author(s)
      Ueki, N., Ide, T., Mochiji, S., Kobayashi, Y., Tokutsu, R., Ohnishi, N., Yamaguchi, K., Shigenobu, S., Tanaka, K., Minagawa, J., Hisabori, T., Hirono, M., Wakabayashi, K.
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 印刷中 Issue: 19 Pages: 5266-5304

    • DOI

      10.1073/pnas.1525538113

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cooperative binding of the outer arm docking complex underlies the regular arrangement of outer arm dynein in the axoneme.2014

    • Author(s)
      Mikito Owa, Akane Furuta, Jiro Usukura, Fumio Arisaka, Stephen M. King, George B. Witman, Ritsu Kamiya, Ken-ichi Wakabayashi
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 111(26) Issue: 26 Pages: 9455-9460

    • DOI

      10.1073/pnas.1403101111

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 緑藻の光行動における鞭毛運動調節:クラミドモナスとボルボックス2017

    • Author(s)
      植木紀子、若林憲一
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島・鹿児島大学群元キャンパスンパス
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] クラミドモナスのγ-tubulin 優性突然変異 bld132017

    • Author(s)
      中澤友紀 、堀井麻央 、渡辺早紀 、大月萌子 、苗加彰 、若林憲一 、廣野雅文
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      神戸・神戸国際会議場
    • Year and Date
      2017-01-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] クラミドモナスにおける走光性の正負と「利き鞭毛」の関係2017

    • Author(s)
      中島昌子 、植木紀子 、若林憲一
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      神戸・神戸国際会議場
    • Year and Date
      2017-01-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] ボルボックスのカルシウム依存的繊毛打逆転と走光性2017

    • Author(s)
      植木紀子、若林憲一
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      神戸・神戸国際会議場
    • Year and Date
      2017-01-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] Photo-behavior in green algae: how light modulates flagellar motility2017

    • Author(s)
      Masako Nakajima, Noriko Ueki, Ken-ichi Wakabayashi
    • Organizer
      光合成科学:エネルギーとバイオマス
    • Place of Presentation
      横浜・東工大すずかけホール
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Modulation of flagellar motility during phototactic turning in Chlamydomonas2016

    • Author(s)
      Masako Nakajima, Noriko Ueki, Ken-ichi Wakabayashi
    • Organizer
      The 28th CDB Meeting: Cilia and Centrosomes: Current Advances and Future Directions
    • Place of Presentation
      神戸・理研CDB
    • Year and Date
      2016-11-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The molecular toolbox for building axonemal microtubules2016

    • Author(s)
      Mikito Owa, Takayuki Uchihashi, Haruaki Yanagisawa, Takashi Yamano, Hiro Iguchi, Hideya Fukuzawa, Ken-ichi Wakabayashi, Toshio Ando, Masahide Kikkawa
    • Organizer
      The 28th CDB Meeting: Cilia and Centrosomes: Current Advances and Future Directions
    • Place of Presentation
      神戸・理研CDB
    • Year and Date
      2016-11-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 緑藻クラミドモナスの走光性発現メカニズム2016

    • Author(s)
      若林憲一
    • Organizer
      遺伝研研究会「水圏微生物のエネルギー利用戦略とその相互作用に関する研究会」
    • Place of Presentation
      三島・遺伝研
    • Year and Date
      2016-11-03
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Visualization and quantification of the redox potential in the Chlamydomonas flagella2016

    • Author(s)
      Yuta Nishimaki, Kazunori Sugiura, Toru Hisabori, Ken-ichi Wakabayashi
    • Organizer
      The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
    • Place of Presentation
      京都・京都国際会館
    • Year and Date
      2016-06-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii2016

    • Author(s)
      Noriko Ueki, Takahiro Ide, Shota Mochiji, Yuki Kobayashi, Ryutaro Tokutsu, Norikazu Ohnishi, Katushi Yamaguchi, Shuji Shigenobu, Kan Tanaka, Jun Minagawa, Toru Hisabori, Masafumi Hirono, Ken-ichi Wakabayashi
    • Organizer
      The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
    • Place of Presentation
      京都・京都国際会館
    • Year and Date
      2016-06-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Isolation of a non-phototactic Chlamydomonas mutant that shows alternate flagellar beatings2016

    • Author(s)
      Akinori Koitabashi, Masako Nakajima, Noriko Ueki, Kogiku Shiba, Takashi Ymano, Hideya Fukuzawa, Inaba Kazuo, Toru Hisabori, Ken-ichi Wakabayashi
    • Organizer
      The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
    • Place of Presentation
      京都・京都国際会館
    • Year and Date
      2016-06-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of the agg1 mutation responsible for negative phototaxis in a "wild-type" strain of Chlamydomonas reinhardtii2016

    • Author(s)
      Takahiro Ide, Shota Mochuji, Noriko Ueki, Katsushi Yamaguchi, Shuji Shigenobu, Masafumi Hirono, Ken-ichi Wakabayashi
    • Organizer
      The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
    • Place of Presentation
      京都・京都国際会館
    • Year and Date
      2016-06-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 緑藻クラミドモナスとボルボックスの走光性のしくみ2016

    • Author(s)
      若林憲一
    • Organizer
      第34回エアロアクアバイオメカニズム学会定例講演会
    • Place of Presentation
      東京・東京工業大学大岡山キャンパス
    • Year and Date
      2016-03-23
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 緑藻クラミドモナスの走光性メカニズム - 光受容と鞭毛運動の連関 -2016

    • Author(s)
      若林憲一
    • Organizer
      日本プランクトン学会春季シンポジウム「光環境を巡る植物プランクトンの生理生態学的最前線」
    • Place of Presentation
      東京・東京大学本郷キャンパス
    • Year and Date
      2016-03-14
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Calcium-dependent photobehavior of the unicellular green alga Chlamydomonas reinhardtii2015

    • Author(s)
      Ken-ichi Wakabayashi
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸・ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] クラミドモナス走光性符号決定における眼点の役割2015

    • Author(s)
      植木紀子、持地翔太 、井手隆広 、山口勝司 、重信秀治 、得津隆太郎 、大 西紀和、皆川純、廣野雅文、若林憲一
    • Organizer
      日本植物生理学会第56回年会
    • Place of Presentation
      東京農業大学(東京都)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] クラミドモナス走光性符号決定における眼点の役割2015

    • Author(s)
      植木紀子, 持地翔太, 井手隆広, 廣野雅文, 若林憲一
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      学習院大学(東京都)
    • Year and Date
      2015-01-07 – 2015-01-09
    • Related Report
      2014 Research-status Report
  • [Presentation] クラミドモナス走光性符号決定における眼点の役割2014

    • Author(s)
      若林憲一 、持地翔太 、植木紀子 、井手隆広 、山口勝司 、重信秀治 、得津隆太郎 、大 西紀和、皆川純、廣野雅文
    • Organizer
      第11回クラミドモナス研究会
    • Place of Presentation
      かるぽーと(高知県)
    • Year and Date
      2014-10-03 – 2014-10-04
    • Related Report
      2014 Research-status Report
  • [Presentation] 緑藻ボルボックス目における光反応行動調節機構2014

    • Author(s)
      植木紀子、箕浦高子、若林憲一
    • Organizer
      第11回クラミドモナス研究会
    • Place of Presentation
      かるぽーと(高知県)
    • Year and Date
      2014-10-03 – 2014-10-04
    • Related Report
      2014 Research-status Report
  • [Presentation] Sign-Control of Chlamydomonas Phototaxis by Redox Poise2014

    • Author(s)
      Ken-ichi Wakabayashi
    • Organizer
      16th International Congress on Photobiology
    • Place of Presentation
      コルドバ国立大学
    • Year and Date
      2014-09-08 – 2014-09-12
    • Related Report
      2014 Research-status Report
    • Invited
  • [Remarks] 久堀・若林研究室ウェブサイト

    • URL

      http://www.res.titech.ac.jp/~junkan/Hisabori_HomePage/index.html

    • Related Report
      2016 Annual Research Report 2015 Research-status Report 2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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