• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analyses of nuclear fusion during plant reproduction using gametophyte cell-specific gene induction systems

Research Project

Project/Area Number 26650094
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionNiigata University

Principal Investigator

Nishikawa Shuh-ichi  新潟大学, 自然科学系, 教授 (10252222)

Research Collaborator KAMEI Yasuhiro  基礎生物学研究所, 生物機能解析センター, 特任准教授
URAWA Hiroko  岐阜聖徳学園大学, 教育学部, 准教授
YAMAGUCHI Yuki  新潟大学, 大学院自然科学研究科, 大学院生
WADA Satomi  新潟大学, 大学院自然科学研究科, 大学院生
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords核膜融合 / 有性生殖 / 遺伝子発現誘導系 / シロイヌナズナ / 配偶体 / 雌性配偶体 / 遺伝子発現誘導
Outline of Final Research Achievements

In this study, we developed a new gene induction method, which allows cell-specific gene induction in Arabidopsis female gametophytes using heat shock-induced Cre-loxP recombination and female gametophyte-specific promoters. Female gametophyte-specific gene expressions were induced by heat treatment of flower buds of constructed transgenic lines. We also showed induction of cell-specific gene expression by irradiating isolated female gametophytes with the infrared laser. The developed method will be a good tool to analyze functions of proteins involved in the fusion of polar nuclei during female gametophyte development.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (13 results)

All 2016 2015 2014

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 5 results,  Invited: 3 results)

  • [Journal Article] Rapid Elimination of the Persistent Synergid through a Cell Fusion Mechanism.2015

    • Author(s)
      丸山大輔、Ronny Völz、竹内英憲、森稔幸、井川智子、栗原大輔、河島友和、植田美那子、伊藤正樹、梅田正明、西川周一、Rita Groß-Hardt、東山哲也
    • Journal Title

      Cell

      Volume: 未定 Issue: 4 Pages: 907-918

    • DOI

      10.1016/j.cell.2015.03.018

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] BiP3 supports the early stages of female gametogenesis in the absence of BiP1 and BiP2 in Arabidopsis thaliana.2015

    • Author(s)
      Daisuke Maruyama, Toshiya Endo, Shuh-ichi Nishikawa
    • Journal Title

      Plant Signaling & Behavior

      Volume: 10 Issue: 7 Pages: e1035853-e1035853

    • DOI

      10.1080/15592324.2015.1035853

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The Plant Organelles Database 3 (PODB3) update 2014: integrating electron micrographs and new options for plant organelle research.2014

    • Author(s)
      Mano S, Nakamura T, Kondo M, Miwa T, Nishikawa S, Mimura T, Nagatani A, Nishimura M.
    • Journal Title

      Plant Cell Physiol.

      Volume: 55 Issue: 1 Pages: e1-e1

    • DOI

      10.1093/pcp/pct140

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Different Sets of ER-Resident J-Proteins Regulate Distinct Polar Nuclear-Membrane Fusion Events in Arabidopsis thaliana.2014

    • Author(s)
      丸山大輔、山本雅也、遠藤斗志也、西川周一
    • Journal Title

      Plant Cell Physiol.

      Volume: 55 Issue: 11 Pages: 1937-1944

    • DOI

      10.1093/pcp/pcu120

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Fertilization-coupled sperm nuclear membrane fusion is required for proper endosperm proliferation in Arabidopsis thaliana2016

    • Author(s)
      西川周一
    • Organizer
      24th International Congress on Sexual Plant Reproduction
    • Place of Presentation
      Tucson Marriott Univesity Park
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Chaperone-dependent nuclear fusion in Arabidopsis reproduction2016

    • Author(s)
      西川周一
    • Organizer
      RISE workshop
    • Place of Presentation
      University of Arizona
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 小胞体分子シャペロンによる 植物有性生殖過程の核膜融合の制御2015

    • Author(s)
      西川周一
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] シロイヌナズナの花粉成熟過程の葯における小胞体分子シャペロンの発現解析2015

    • Author(s)
      宇治周平
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター
    • Year and Date
      2015-09-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シロイヌナズナ小胞体品質管理欠損株は特異的な細胞膜受容体様キナーゼの機能欠損のため花粉成熟が異常となる2015

    • Author(s)
      西川周一
    • Organizer
      第67回日本細胞生物学会大会
    • Place of Presentation
      タワーホール船堀
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 花粉形成過程に必要な受容体キナーゼの機能発現におけるAtERdj3Bの役割の特異性2015

    • Author(s)
      加藤詩織、杉山智之、山本雅也、野元美佳、多田安臣、遠藤斗志也、西川周一
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学世田谷キャンパス(東京都世田谷区)
    • Year and Date
      2015-03-16
    • Related Report
      2014 Research-status Report
  • [Presentation] Roles of ER-resident molecular chaperonesin functions of recepto-like kinases involved in male gametogenesis2015

    • Author(s)
      加藤詩織、杉山智之、山本雅也、野元美佳、多田安臣、遠藤斗志也、西川周一
    • Organizer
      The 2nd International Symposium on Plant Environmental Sensing
    • Place of Presentation
      産業技術総合研究所臨海副都心センター(別館)(東京都江東区)
    • Year and Date
      2015-03-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Chaperone-dependent nuclear membrane fusion in Arabidopsis fertilization2014

    • Author(s)
      西川周一、丸山大輔、山本雅也、東山哲也、遠藤斗志也
    • Organizer
      KAAB International Symposium 2014
    • Place of Presentation
      新潟大学(新潟県新潟市)
    • Year and Date
      2014-09-29
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 小胞体品質管理による花粉成熟過程の解析2014

    • Author(s)
      杉山智之、山本雅也、遠藤斗志也、西川周一
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県川崎市)
    • Year and Date
      2014-09-13
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi