| Project/Area Number |
26650095
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NISHIHAMA Ryuichi 京都大学, 大学院生命科学研究科, 講師 (70283455)
YAMAOKA Shohei 京都大学, 大学院生命科学研究科, 助教 (00378770)
ISHIZAKI Kimitsune 神戸大学, 大学院理学研究科, 准教授 (00452293)
SUGANO Shigeo 京都大学, 大学院理学研究科, 研究員 (60726313)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 植物発生学 / 植物環境応答 / ゼニゴケ / ゲノム編集 / ゲノミクス / CRISPR/Cas / 植物分子遺伝学 / 転写制御 / 植物発生制御 / 転写因子 / 苔類ゼニゴケ |
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| Outline of Final Research Achievements |
Land plants acquired a system to coordinate developmental program with environment during evolution. Transcriptional regulation is one of the major mechanisms for the adaptive growth and development in plants. As a basal land plant, the liverwort Marchantia polymorpha is an attractive model organism with haploid-dominant life cycle. In addition, sophisticated experimental systems such as efficient genetic transformation and genome information have been developed in M. polymorpha. We deduced the entire set of genes for transcription factors from the draft genome sequences of M. polymorpha. To experimentally analyze gene function efficiently, the CRISPR/Cas9-mediated genome editing is a simple and straightforward method to disrupt genes of interest. We significantly improved the efficiency of genome editing by optimizing Cas9 expression and CRISPR design. Simple, rapid and efficient mutagenesis by CRISPR/Cas9 enabled systematic analysis of transcription factors in M. polymorpha.
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