Development of an inducible gene expression system with a plant UV-B receptor and pulse laser

• Project/Area Number: 26650096
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: Aoyama Takashi 京都大学, 化学研究所, 教授 (80202498)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 転写誘導系 / 紫外線 / シロイヌナズナ / UVレーザー / UV-B受容体 / 誘導系 / 形質転換植物 / 転写因子 / UV / 植物転写因子 / 超短パルスレーザー

Outline of Final Research Achievements

The Arabidopsis UV-B receptor UVR8 and UV pulse laser are utilized for the purpose of developing an artificial induction system of gene expression with a high spatiotemporal resolution. UVR8 forms a homodimer in the nucleus under normal plant growth conditions, and reversibly dissociates to interact with other regulatory proteins in response to UV-B irradiation. Taking advantage of these characteristics of UVR8, construction of an UV-inducible transcription system was attempted by combining molecular functions of LexA, the transcription activation domain of VP16, the SRDX motif, and the WD40 domain of COP1.

Research Products

• [Int'l Joint Research] 北京大学(中国)
• [Journal Article] RNA binding proteins RZ-1B and RZ-1C play critical roles in regulating pre-mRNA splicing and gene expression during development in Arabidopsis. (2016)
  • Author(s): Wu Z, Zhu D, Lin X, Miao J, Gu L, Deng X, Yang Q, Sun K, Zhu D, Cao X, Tsuge T, Dean C, Aoyama T, Gu H, Qu LJ.
  • Journal Title: Plant Cell Volume: 28 Issue: 1 Pages: 55-73
  • DOI: 10.1105/tpc.15.00949
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] GLABRA2 directly suppresses basic helix-loop-helix transcription factor genes with diverse functions in root hair development. (2015)
  • Author(s): Lin, Q., Ohashi, Y., Kato, M., Tsuge, T., Gu ,H., Qu, L.-J., and Aoyama, T.
  • Journal Title: Plant Cell Volume: 27 Pages: 2894-2906
  • DOI: 10.1105/tpc.15.00607
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Phosphatidylinositol phosphate 5-kinase genes respond to phosphate deficiency for root hair elongation in Arabidopsis thaliana. (2015)
  • Author(s): Wada, Y., Kusano, H., Tsuge, T., and Aoyama, T.
  • Journal Title: Plant J. Volume: 81 Issue: 3 Pages: 426-437
  • DOI: 10.1111/tpj.12741
  • Peer Reviewed / Open Access
• [Journal Article] Characteristics of a root hair-less line of Arabidopsis thaliana under physiological stresses. (2014)
  • Author(s): Tanaka, N., Kato, M., Tomioka, R., Kurata, R., Fukao, Y., Aoyama, T., and Maeshima, M.
  • Journal Title: Journal of Experimental Botany Volume: 65 Issue: 6 Pages: 1497-1512
  • DOI: 10.1093/jxb/eru014
  • Peer Reviewed / Open Access
• [Journal Article] TRANSLUCENT GREEN, an ERF family transcription factor, controls water balance in Arabidopsis by activating the expression of aquaporin genes. (2014)
  • Author(s): Zhu, D., Wu, Z., Cao, G., Li, J., Wei, J., Tsuge, T., Gu, H., Aoyama, T., and Qu, L.-J.
  • Journal Title: Mol. Plant Volume: 7 Issue: 4 Pages: 601-615
  • DOI: 10.1093/mp/sst152
  • Peer Reviewed
• [Journal Article] Hayashi K, Nakamura S, Fukunaga S, Nishimura T, Jenness MK, Murphy AS, Motose H, Nozaki H, Furutani M, Aoyama T. (2014)
  • Author(s): Auxin transport sites are visualized in planta using fluorescent auxin analogs.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 111 Issue: 31 Pages: 111557-111562
  • DOI: 10.1073/pnas.1408960111
  • Peer Reviewed / Open Access
• [Presentation] Cell Biological Function of the Arabidopsis PLDζ2 (2016)
  • Author(s): 島村亮太, 谷口（山本）幸美、加藤真理子、柘植知彦 、青山卓史
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡
  • Year and Date: 2016-03-18
• [Presentation] Regulatory Roles of PIP5K Genes in Plant Cell Morphogenesis and Their Redundancy (2016)
  • Author(s): 亘真智子、Blanc-Mathieu Romain、加藤真理子、柘植知彦 、青山卓史
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡
  • Year and Date: 2016-03-18
• [Presentation] PIP5K3と他のタイプB PIP5K遺伝子との機能重複に関する解析 (2015)
  • Author(s): 齊藤 涼、和田悠貴香、加藤真理子、柘植知彦、青山卓史
  • Organizer: 日本植物生理学会・2015年度年会
  • Place of Presentation: 東京農業大学、東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] シロイヌナズナ根毛におけるCa2+結合タンパク質PCaP2とホスファチジルイノシトール4,5二リン酸の細胞内動態 (2015)
  • Author(s): 加藤真理子、柘植知彦、前島正義、青山卓史
  • Organizer: 日本植物生理学会・2015年度年会
  • Place of Presentation: 東京農業大学、東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 側方根冠におけるシロイヌナズナPLDDζ2の役割 (2015)
  • Author(s): 島村亮太、谷口（山本）幸美、谷口雅俊、加藤真理子、柘植知彦、林 謙一郎、青山卓史
  • Organizer: 日本植物生理学会・2015年度年会
  • Place of Presentation: 東京農業大学、東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] シロイヌナズナの側方根冠におけるPLDDζ2-PAシグナルの役割 (2014)
  • Author(s): 島村亮太、谷口（山本）幸美、谷口雅俊、加藤真理子、柘植知彦、青山卓史
  • Organizer: 第27回植物脂質シンポジウム
  • Place of Presentation: 静岡市産学交流センター、静岡
  • Year and Date: 2014-11-28 – 2014-11-29
• [Presentation] 根毛伸長時におけるシロイヌナズナCa2+結合タンパク質PCaP2とホスファチジルイノシトール-4,5二リン酸の細胞内動態 (2014)
  • Author(s): 加藤真理子、柘植知彦、前島正義、青山卓史
  • Organizer: 第27回植物脂質シンポジウム
  • Place of Presentation: 静岡市産学交流センター、静岡
  • Year and Date: 2014-11-28 – 2014-11-29
• [Presentation] シロイヌナズナの根毛伸長におけるPIP5K遺伝子の役割 (2014)
  • Author(s): 和田悠貴香、草野博彰、亘真智子、斉藤 涼、加藤真理子、柘植知彦、青山卓史
  • Organizer: 第27回植物脂質シンポジウム
  • Place of Presentation: 静岡市産学交流センター、静岡
  • Year and Date: 2014-11-28 – 2014-11-29
• [Book] Plant Cell Wall Patterning and Cell Shape (Ed., Fukuda H.) (2014)
  • Author(s): Kusano, H., Tominaga, R., Wada, T., Kato, M., and Aoyama, T.
  • Total Pages: 432
  • Publisher: Wiley-Blackwell