Identification of genome mutation that induce C4 evolution by Quantitative Trait Locus (QTL) analysis

• Project/Area Number: 26650097
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kwansei Gakuin University (2015-2016); Nara Institute of Science and Technology (2014)
• Principal Investigator: Munekage Yuri 関西学院大学, 理工学部, 准教授 (30423247)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 光合成 / 遺伝学的解析

Outline of Final Research Achievements

To identify the genome mutation that induce C4 evolution, we performed Quantitative Trait Locus (QTL) analysis in F2 progeny of hybrids between C3-C4 intermediate species and C4-like species of genus Flaveria. Our results suggested that expression level of C4 cycle enzymes was regulated by different factors that regulate expression region of C4 cycle enzymes or Kranz anatomy. We also succeeded to visualize 26S and 5s rDNA as a chromosome marker in genus Flaveria by Fluorescence in situ hybridization.

Research Products

• [Journal Article] C4 型光合成の進化過程からC4 化の鍵を探る (2015)
  • Author(s): 宗景ゆり
  • Journal Title: 光合成研究 Volume: 25 Pages: 3-3
  • Peer Reviewed / Open Access
• [Presentation] NADP-ME型C4 Flaveria bidentisにおける循環型電子伝達で機能するNDH複合体及びPGR5-PGRL1複合体の光合成への寄与 (2017)
  • Author(s): 小林加奈、中村有哉、森川かおる、横田明穂、谷口（山本）幸美、宗景（中島）ゆり
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県、鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] Flaveria属C4種の本葉の発達過程におけるFbDOFの発現パターン解析 (2017)
  • Author(s): 奥殿健、谷口（山本）幸美、宗景（中島）ゆり
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県、鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] C4種Flaveria bidentisにおけるRETICULATE-RELATED3の局在解析 (2017)
  • Author(s): 花田裕昭、谷口（山本）幸美、西村健司、坂本亘、古本強、宗景（中島）ゆり
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県、鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] キク科Flaveria属形質転換系と遺伝子発現誘導系の確立 (2016)
  • Author(s): 岡 美慧, 谷口 幸美, 宗景 ゆり
  • Organizer: 第68回日本生物工学会大会
  • Place of Presentation: 富山国際会議場（富山県、富山市）
  • Year and Date: 2016-09-28
• [Presentation] Role of cyclic electron transport around photosystem I in NADP-ME type C4 Flaveria. (2016)
  • Author(s): Munekage Y.N
  • Organizer: Satellite workshop on C4 photosynthesis.
  • Place of Presentation: Dusseldorf (Germany)
  • Year and Date: 2016-08-05
  • Int'l Joint Research / Invited
• [Presentation] Light harvesting and chloroplast electron transport in NADP-malic enzyme type C4 plants (2016)
  • Author(s): Munekage Y.N
  • Organizer: C4:50: Photosynthesis Conference
  • Place of Presentation: Canberra (Australia)
  • Year and Date: 2016-04-10
  • Int'l Joint Research / Invited
• [Presentation] Flaveria属植物の解析から見えるC3 型からC4 型光合成への進化モデル (2015)
  • Author(s): 宗景ゆり
  • Organizer: 日本光合成学会
  • Place of Presentation: 岡山国際交流センター（岡山県岡山市）
  • Year and Date: 2015-05-22
  • Invited
• [Presentation] Flaveria属C3-C4中間型F.floridanaとC4様型F.browniiの交雑F2集団を用いたC4型進化過程の遺伝学的解析 (2015)
  • Author(s): 宗景（中島）ゆり、井上史生、谷口幸美、横田明穂
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 東京農業大学世田谷キャンパス（東京都世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Flaveria属植物の解析から見えるC4型進化モデル (2014)
  • Author(s): 宗景（中島）ゆり
  • Organizer: 日本植物学会
  • Place of Presentation: 明治大学生田キャンパス（神奈川県川崎市）
  • Year and Date: 2014-09-12 – 2014-09-14
  • Invited