Analysis of Cdc25-like phosphatases involved in cytokinin-induced cell division

• Project/Area Number: 26650099
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: UMEDA Masaaki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (80221810)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 細胞周期 / サイクリン依存性キナーゼ / 植物ホルモン / ゼニゴケ / 発生・分化 / シグナル伝達 / 植物 / 蛋白質 / 酵素

Outline of Final Research Achievements

Cdc25 is a protein phosphatase that plays an important role in controlling the G2-to-M transition in animals and yeasts. However, no orthologous gene has been identified in plants. In this study, we aimed to characterize Cdc25-like phosphatases in plants. Our analyses using Arabidopsis thaliana and Marchantia polymorpha suggested that plant Cdc25-like phosphatases are associated with cell cycle progression from the G1 to the S phase, and that they are originated from the same ancestor as animal and yeast Cdc25.

Research Products

• [Journal Article] Cytrap marker systems for in vivo visualization of cell cycle progression in Arabidopsis (2016)
  • Author(s): Sugamata Aki, S. and Umeda, M.
  • Journal Title: Methods Mol. Biol. Volume: 1370 Pages: 51-57
  • DOI: 10.1007/978-1-4939-3142-2_4
  • ISBN: 9781493931415, 9781493931422
• [Journal Article] Cyclin-dependent kinase-activating kinases CDKD;1 and CDKD;3 are essential for preserving mitotic activity in Arabidopsis thaliana (2015)
  • Author(s): Takatsuka, H., Umeda-Hara, C. and Umeda, M.
  • Journal Title: Plant J. Volume: 82 Issue: 6 Pages: 1004-1017
  • DOI: 10.1111/tpj.12872
  • NAID: 120006537393
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Transcriptional repression by MYB3R proteins regulates plant organ growth. (2015)
  • Author(s): Kobayashi K, Suzuki T, Iwata E, Nakamichi N, Suzuki T, Chen P, Ohtani M, Ishida T, Hosoya H, Müller S, Leviczky T, Pettkó-Szandtner A, Darula Z, Iwamoto A, Nomoto M, Tada Y, Higashiyama T, Demura T, Doonan JH, Hauser MT, Sugimoto K, Umeda M, Magyar Z, Bögre L, Ito M
  • Journal Title: EMBO Journal Volume: 34 Issue: 15 Pages: 1992-2007
  • DOI: 10.15252/embj.201490899
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Epigenetic control of cell division and cell differentiation in the root apex (2015)
  • Author(s): Takatsuka, H. and Umeda, M.
  • Journal Title: Front. Plant Sci. Volume: 6 Pages: 1178-1178
  • DOI: 10.3389/fpls.2015.01178
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A dual-color marker system for in vivo visualization of cell cycle progression in Arabidopsis (2014)
  • Author(s): Yin, K., Ueda, M., Takagi, H., Kajihara, T., Sugamata Aki, S., Nobusawa, T., Umeda-Hara, M. Umeda
  • Journal Title: Plant J. Volume: 80 Issue: 3 Pages: 541-552
  • DOI: 10.1111/tpj.12652
  • NAID: 120006537394
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cytokinins promote onset of endoreplication by controlling cell cycle machinery (2014)
  • Author(s): Takahashi, N. and Umeda, M.
  • Journal Title: Plant Signal. Behav. Volume: 9 Issue: 8 Pages: e29396-e29396
  • DOI: 10.4161/psb.29396
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Maintenance of genome integrity in root stem cells under DNA stress (2016)
  • Author(s): 高橋直紀、藤本啓介、梅田正明
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大（岩手県盛岡市）
  • Year and Date: 2016-03-18
  • Invited
• [Presentation] 根端分裂組織のサイズ制御機構 (2015)
  • Author(s): 高橋直紀、丸池加奈子、高塚大知、梅田正明
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ・新潟コンベンションセンター（新潟県新潟市中央区）
  • Year and Date: 2015-09-06
  • Invited
• [Presentation] Functional analysis of cytokinin response regulators in Marchantia polymorpha (2015)
  • Author(s): 安喜（菅又）詩織、三神達也、西浜竜一、河内孝之、梅田正明
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大（東京都世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Functional analysis of cytokinin response regulators in Marchantia polymorpha (2014)
  • Author(s): 安喜（菅又）詩織、三神達也、西浜竜一、河内孝之、梅田正明
  • Organizer: Marchantia Workshop 2014
  • Place of Presentation: 神戸大（兵庫県神戸市）
  • Year and Date: 2014-12-08 – 2014-12-10
• [Presentation] Visualization of cell cycle progression using S/G2- and G2/M-specific markers (2014)
  • Author(s): 安喜（菅又）詩織、高木瞳、Ke Yin、梅田（原）千景、植田美那子、梅田正明
  • Organizer: Auxins and Cytokinins in Plant Development International Symposium 2014
  • Place of Presentation: プラハ（チェコ）
  • Year and Date: 2014-06-29 – 2014-07-04
• [Remarks] 植物成長制御研究室ホームページ
  • URL: http://bsw3.naist.jp/umeda/index.html