| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
Plant MYB transcription factor EPR1 can repress transcription in yeasts and animal cells as well as in plant cells. In this study, we aim to identify the transcriptional repression motifs of EPR1 in plants, yeasts, and animals, respectively and clarify the molecular mechanisms of transcriptional repression conserved in eukaryotes. Our analysis showed that EPR1 has two transcriptional repression motifs. The domain D in the C-terminal region cannot suppress the transcriptional activity in animal cells, while domain A in the N-terminal region suppress the transcriptional activity in plants, yeasts, and animal cells. Transcriptional repression of EPR1 across kingdoms might depend on two independent motifs with different mechanisms.
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