Development of transcriptional study using new transcriptional repression motif ERD
| Project/Area Number |
26650101
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 植物 / 発現制御 |
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| Outline of Final Research Achievements |
Plant MYB transcription factor EPR1 can repress transcription in yeasts and animal cells as well as in plant cells. In this study, we aim to identify the transcriptional repression motifs of EPR1 in plants, yeasts, and animals, respectively and clarify the molecular mechanisms of transcriptional repression conserved in eukaryotes. Our analysis showed that EPR1 has two transcriptional repression motifs. The domain D in the C-terminal region cannot suppress the transcriptional activity in animal cells, while domain A in the N-terminal region suppress the transcriptional activity in plants, yeasts, and animal cells. Transcriptional repression of EPR1 across kingdoms might depend on two independent motifs with different mechanisms.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] DELLA-GAF1 complex is a main component in gibberellin feedback regulation of GA20ox2 in Arabidopsis2017
Author(s)
Fukazawa, J., Mori, M., Watanabe, S., Miyamoto, C., Ito, T. and Takahashi, Y.
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Journal Title
Plant Physiol.
Volume: 印刷中
Related Report
Peer Reviewed
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[Journal Article] Binding of GID1 to DELLAs Promotes Dissociation of GAF1 from DELLA in GA Dependent Manner2015
Author(s)
Fukazawa, J., Ito, T., Yoshida, M., Kamiya, Y., Yamaguchi, S., and Takahashi, Y.
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Journal Title
Plant Signaling and Behavior
Volume: 10
Issue: 10
Pages: e1052923-e1052923
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] GAF1, A DELLA INTERACTING PROTEIN, REGULATES GIBBERELLIN HOMEOSTASIS AND SIGNALING2014
Author(s)
Fukazawa J, Fujiki T, Mori M, Miyamoto C, Mishima Y, Kamiya Y, Yamaguchi S, Takahashi Y.
Organizer
25th International Conference on Arabidopsis Research
Place of Presentation
Vancouver, Canada
Year and Date
2014-07-28 – 2014-08-01
Related Report
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[Presentation] Scaffold function of Ca2+-dependent protein kinase: NtCDPK1 transfers 14-3-3 to the substrate RSG after phosphorylation2014
Author(s)
Ito, T., Nakata, M., Fukazawa, J., Ishida, S., and Takahashi, Y.
Organizer
Plant Biology 2014
Place of Presentation
Portland, Oregon, USA.
Year and Date
2014-07-12 – 2014-07-26
Related Report
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