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Mechanistic studies on the functional switching of plant LysM receptors from defense to symbiotic responses.

Research Project

Project/Area Number 26650104
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionMeiji University

Principal Investigator

Shibuya Naoto  明治大学, 農学部, 教授 (70350270)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords植物免疫 / 共生 / LysM受容体 / シグナル伝達 / 自己リン酸化 / 構造・機能解析 / 構造・機能 / キチン
Outline of Final Research Achievements

Symbiosis-inducing ability of the kinase domains of CERK1/NFR1 receptor-like kinases relies on the presence of YAQ sequence in their kinase domains. To clarify the background of such “switching” of the downstream responses, autophosphorylation sites of the kinase domain of CERK1 or YAQ-CERK1 were analyzed. Interestingly, no difference was observed between autophosphorylation sites of these proteins, indicating that the structural changes induced by the presence of YAQ sequence itself plays critical roles for the “switching” of the downstream responses.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (18 results)

All 2016 2015 2014

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (13 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Book (1 results)

  • [Journal Article] Quantification of stimulus-induced callose spots on plant materials.2016

    • Author(s)
      M. Kohari, K. Yashima, Y. Desaki, N. Shibuya
    • Journal Title

      Plant Biotechnol.

      Volume: 33 Pages: 11-17

    • NAID

      130005146720

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Chitin-mediated plant–fungal interactions: catching, hiding and handshaking2015

    • Author(s)
      Tomonori Shinya, Tomomi Nakagawa, Hanae Kaku, Naoto Shibuya
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 26 Pages: 64-71

    • DOI

      10.1016/j.pbi.2015.05.032

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] キチンオリゴ糖は受容体のサンドイッチ型ダイマー形成を介して植物免疫を活性化する2015

    • Author(s)
      賀来華江、渋谷直人
    • Journal Title

      化学と生物

      Volume: 53 Pages: 726-727

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bifunctional plant receptor, OsCERK1, regulates both chitin-triggered immunity and arbuscular mycorrhizal symbiosis in rice.2014

    • Author(s)
      K. Miyata, T. Kozaki, Y. Kouzai, K. Ozawa, K. Ishii, E. Asamizu, Y. Okabe, Y. Umehara, A. Miyamoto, Y. Kobae, K. Akiyama, H. Kaku, Y. Nishizawa, N. Shibuya, T. Nakagawa
    • Journal Title

      Plant and Cell Physiology

      Volume: 55 Issue: 11 Pages: 1859-1863

    • DOI

      10.1093/pcp/pcu129

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] シロイヌナズナのユビキチンリガーゼPUB4はCERK1との相互作用を介して免疫応答を制御する2016

    • Author(s)
      出崎能丈、高橋昌平、小泉春樹、三浦駿希、八嶋航平、石橋裕子、紀藤圭治、鳴坂真理、鳴坂義弘、賀来華江、渋谷直人
    • Organizer
      平成28年度日本植物病理学会大会
    • Place of Presentation
      岡山市
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] シロイヌナズナキチン受容体キナーゼCERK1の自己リン酸化部位の機能解析2016

    • Author(s)
      鈴木丸陽、須藤健吉、渋谷匡俊、島田日加瑠、元山記子、高橋昌平、吉田一誠、松井 紗樹、中島正登、大西美帆子、紀藤圭治、出崎能丈、賀来華江、渋谷直人
    • Organizer
      平成28年度日本植物病理学会大会
    • Place of Presentation
      岡山市
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] Identification and functional analysis of phosphorylation sites in Arabidopsis CERK1.2016

    • Author(s)
      K. Suto, M. Suzuki, M. Shibuya, H. Shimada, N. Motoyama, S., Takahashi, I. Yoshida, S. Matsui, M. Nakashima, M. Ohnishi, K. Kito, Y., Desaki, H. Kaku, N. Shibuya
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      盛岡市
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] An E3 ubiquitin ligase, PUB4, regulates immune signaling through the interaction with Arabidopsis CERK1.2016

    • Author(s)
      S. Takahashi, H. Koizumi, T. Miura, K. Yashima, Y. Ishibashi, K. Kito, M. Narusaka, Y. Narusaka, Y. Desaki, H. Kaku, N. Shibuya
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      盛岡市
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Evaluation of membrane traffic components involved in the MAMP-induced callose deposition in Arabidopsis.2016

    • Author(s)
      Kohei Yashima, Masaki Kohari, Takashi Ueda, Ikuko Hara-Nishimura, Yoshitake Desaki, Hanae Kaku, Naoto Shibuya.
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      盛岡市
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Membrane traffic components involved in MAMP-triggered callose accumulation in Arabidopsis.2015

    • Author(s)
      Y. Desaki, K. Yashima, M.Kohari, T. Ueda, H. Kaku, N. Shibuya.
    • Organizer
      36th New Phytologist Symposium, “Cell biology at the plant–microbe interface”
    • Place of Presentation
      ミュンヘン市
    • Year and Date
      2015-11-29
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular mechanisms of chitin recognition and immune signaling by LysM-receptors.2015

    • Author(s)
      H. Kaku, N. Shibuya
    • Organizer
      11th Japan-US seminar: Molecular contact points in host-pathogen co-evolution.
    • Place of Presentation
      高松市
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Regulation of immune signaling by an E3 ubiquitin ligase that interacts with Arabidopsis CERK1.2015

    • Author(s)
      Y. Desaki, S. Takahashi, K. yashima, H. Koizumi, T. Miura, H. Ishibashi, K. Kito, M. Narusaka, Y. Narusaka, H. Kaku, . Shibuya.
    • Organizer
      11th Japan-US seminar: Molecular contact points in host-pathogen co-evolution.
    • Place of Presentation
      高松市
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] キチン受容体を介した植物免疫制御機構2014

    • Author(s)
      渋谷直人
    • Organizer
      日本応用糖質科学会平成26年度大会
    • Place of Presentation
      新潟県新潟市
    • Year and Date
      2014-09-24 – 2014-09-26
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] ネ受容体型キナーゼOsCERK1による共生応答と防御応答の選択2014

    • Author(s)
      宮田佳奈・ 香西雄介・小澤憲二郎・小八重善裕・賀来華江・西澤洋子・渋谷直人, 中川知己
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      川崎市
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Research-status Report
  • [Presentation] A SINGLE LysM RECEPTOR KINASE, RICE OsCERK1, SWITCHES REJECTION OR ACCEPTANCE OF INFECTING MICROBES.2014

    • Author(s)
      Tomomi Nakagawa, Kana Miyata, Toshinori Kozaki, Yusuke Kouzai, Kenjirou Ozawa, Kazuo Ishii, Erika Asamizu, Yoshihiro Okabe, Yosuke Umehara, Ayano Miyamoto, Yoshihiro Kobae, Kohki Akiyama, Hanae Kaku, Yoko Nishizawa, Naoto Shibuya
    • Organizer
      第16回植物・微生物相互作用の分子機構に関する国際シンポジウム
    • Place of Presentation
      ギリシャ、ロードス市
    • Year and Date
      2014-07-06 – 2014-07-10
    • Related Report
      2014 Research-status Report
  • [Presentation] The role of a LysM receptor-like kinase, OsNFR5 for root mycorrhization in Oryza sativa.2014

    • Author(s)
      Kana Miyata, Yusuke Kouzai, Kenjirou Ozawa, Yoshihiro Kobae, Hanae Kaku, Yoko Nishizawa, Naoto Shibuya, Tomomi Nakagawa
    • Organizer
      第16回植物・微生物相互作用の分子機構に関する国際シンポジウム
    • Place of Presentation
      ギリシャ、ロードス市
    • Year and Date
      2014-07-06 – 2014-07-10
    • Related Report
      2014 Research-status Report
  • [Presentation] Ligand recognition and membrane signaling by plant chitin receptors2014

    • Author(s)
      Naoto Shibuya
    • Organizer
      Networks in Immunity Workshop
    • Place of Presentation
      米国、デービス市
    • Year and Date
      2014-04-01 – 2014-04-03
    • Related Report
      2014 Research-status Report
    • Invited
  • [Book] The Lotus japonicus genome2014

    • Author(s)
      T. Nakagawa, S. Okazaki, N. Shibuya
    • Total Pages
      267
    • Publisher
      Springer
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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