Mechanistic studies on the functional switching of plant LysM receptors from defense to symbiotic responses.

• Project/Area Number: 26650104
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Meiji University
• Principal Investigator: Shibuya Naoto 明治大学, 農学部, 教授 (70350270)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 植物免疫 / 共生 / LysM受容体 / シグナル伝達 / 自己リン酸化 / 構造・機能解析 / 構造・機能 / キチン

Outline of Final Research Achievements

Symbiosis-inducing ability of the kinase domains of CERK1/NFR1 receptor-like kinases relies on the presence of YAQ sequence in their kinase domains. To clarify the background of such “switching” of the downstream responses, autophosphorylation sites of the kinase domain of CERK1 or YAQ-CERK1 were analyzed. Interestingly, no difference was observed between autophosphorylation sites of these proteins, indicating that the structural changes induced by the presence of YAQ sequence itself plays critical roles for the “switching” of the downstream responses.

Research Products

• [Journal Article] Quantification of stimulus-induced callose spots on plant materials. (2016)
  • Author(s): M. Kohari, K. Yashima, Y. Desaki, N. Shibuya
  • Journal Title: Plant Biotechnol. Volume: 33 Pages: 11-17
  • NAID: 130005146720
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Chitin-mediated plant–fungal interactions: catching, hiding and handshaking (2015)
  • Author(s): Tomonori Shinya, Tomomi Nakagawa, Hanae Kaku, Naoto Shibuya
  • Journal Title: Current Opinion in Plant Biology Volume: 26 Pages: 64-71
  • DOI: 10.1016/j.pbi.2015.05.032
  • Peer Reviewed / Open Access
• [Journal Article] キチンオリゴ糖は受容体のサンドイッチ型ダイマー形成を介して植物免疫を活性化する (2015)
  • Author(s): 賀来華江、渋谷直人
  • Journal Title: 化学と生物 Volume: 53 Pages: 726-727
  • Peer Reviewed
• [Journal Article] Bifunctional plant receptor, OsCERK1, regulates both chitin-triggered immunity and arbuscular mycorrhizal symbiosis in rice. (2014)
  • Author(s): K. Miyata, T. Kozaki, Y. Kouzai, K. Ozawa, K. Ishii, E. Asamizu, Y. Okabe, Y. Umehara, A. Miyamoto, Y. Kobae, K. Akiyama, H. Kaku, Y. Nishizawa, N. Shibuya, T. Nakagawa
  • Journal Title: Plant and Cell Physiology Volume: 55 Issue: 11 Pages: 1859-1863
  • DOI: 10.1093/pcp/pcu129
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] シロイヌナズナのユビキチンリガーゼPUB4はCERK1との相互作用を介して免疫応答を制御する (2016)
  • Author(s): 出崎能丈、高橋昌平、小泉春樹、三浦駿希、八嶋航平、石橋裕子、紀藤圭治、鳴坂真理、鳴坂義弘、賀来華江、渋谷直人
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山市
  • Year and Date: 2016-03-21
• [Presentation] シロイヌナズナキチン受容体キナーゼCERK1の自己リン酸化部位の機能解析 (2016)
  • Author(s): 鈴木丸陽、須藤健吉、渋谷匡俊、島田日加瑠、元山記子、高橋昌平、吉田一誠、松井 紗樹、中島正登、大西美帆子、紀藤圭治、出崎能丈、賀来華江、渋谷直人
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山市
  • Year and Date: 2016-03-21
• [Presentation] Identification and functional analysis of phosphorylation sites in Arabidopsis CERK1. (2016)
  • Author(s): K. Suto, M. Suzuki, M. Shibuya, H. Shimada, N. Motoyama, S., Takahashi, I. Yoshida, S. Matsui, M. Nakashima, M. Ohnishi, K. Kito, Y., Desaki, H. Kaku, N. Shibuya
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡市
  • Year and Date: 2016-03-18
• [Presentation] An E3 ubiquitin ligase, PUB4, regulates immune signaling through the interaction with Arabidopsis CERK1. (2016)
  • Author(s): S. Takahashi, H. Koizumi, T. Miura, K. Yashima, Y. Ishibashi, K. Kito, M. Narusaka, Y. Narusaka, Y. Desaki, H. Kaku, N. Shibuya
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡市
  • Year and Date: 2016-03-18
• [Presentation] Evaluation of membrane traffic components involved in the MAMP-induced callose deposition in Arabidopsis. (2016)
  • Author(s): Kohei Yashima, Masaki Kohari, Takashi Ueda, Ikuko Hara-Nishimura, Yoshitake Desaki, Hanae Kaku, Naoto Shibuya.
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡市
  • Year and Date: 2016-03-18
• [Presentation] Membrane traffic components involved in MAMP-triggered callose accumulation in Arabidopsis. (2015)
  • Author(s): Y. Desaki, K. Yashima, M.Kohari, T. Ueda, H. Kaku, N. Shibuya.
  • Organizer: 36th New Phytologist Symposium, “Cell biology at the plant–microbe interface”
  • Place of Presentation: ミュンヘン市
  • Year and Date: 2015-11-29
  • Int'l Joint Research
• [Presentation] Molecular mechanisms of chitin recognition and immune signaling by LysM-receptors. (2015)
  • Author(s): H. Kaku, N. Shibuya
  • Organizer: 11th Japan-US seminar: Molecular contact points in host-pathogen co-evolution.
  • Place of Presentation: 高松市
  • Year and Date: 2015-10-26
  • Int'l Joint Research / Invited
• [Presentation] Regulation of immune signaling by an E3 ubiquitin ligase that interacts with Arabidopsis CERK1. (2015)
  • Author(s): Y. Desaki, S. Takahashi, K. yashima, H. Koizumi, T. Miura, H. Ishibashi, K. Kito, M. Narusaka, Y. Narusaka, H. Kaku, . Shibuya.
  • Organizer: 11th Japan-US seminar: Molecular contact points in host-pathogen co-evolution.
  • Place of Presentation: 高松市
  • Year and Date: 2015-10-26
  • Int'l Joint Research
• [Presentation] キチン受容体を介した植物免疫制御機構 (2014)
  • Author(s): 渋谷直人
  • Organizer: 日本応用糖質科学会平成26年度大会
  • Place of Presentation: 新潟県新潟市
  • Year and Date: 2014-09-24 – 2014-09-26
  • Invited
• [Presentation] ネ受容体型キナーゼOsCERK1による共生応答と防御応答の選択 (2014)
  • Author(s): 宮田佳奈・ 香西雄介・小澤憲二郎・小八重善裕・賀来華江・西澤洋子・渋谷直人, 中川知己
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 川崎市
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] A SINGLE LysM RECEPTOR KINASE, RICE OsCERK1, SWITCHES REJECTION OR ACCEPTANCE OF INFECTING MICROBES. (2014)
  • Author(s): Tomomi Nakagawa, Kana Miyata, Toshinori Kozaki, Yusuke Kouzai, Kenjirou Ozawa, Kazuo Ishii, Erika Asamizu, Yoshihiro Okabe, Yosuke Umehara, Ayano Miyamoto, Yoshihiro Kobae, Kohki Akiyama, Hanae Kaku, Yoko Nishizawa, Naoto Shibuya
  • Organizer: 第16回植物・微生物相互作用の分子機構に関する国際シンポジウム
  • Place of Presentation: ギリシャ、ロードス市
  • Year and Date: 2014-07-06 – 2014-07-10
• [Presentation] The role of a LysM receptor-like kinase, OsNFR5 for root mycorrhization in Oryza sativa. (2014)
  • Author(s): Kana Miyata, Yusuke Kouzai, Kenjirou Ozawa, Yoshihiro Kobae, Hanae Kaku, Yoko Nishizawa, Naoto Shibuya, Tomomi Nakagawa
  • Organizer: 第16回植物・微生物相互作用の分子機構に関する国際シンポジウム
  • Place of Presentation: ギリシャ、ロードス市
  • Year and Date: 2014-07-06 – 2014-07-10
• [Presentation] Ligand recognition and membrane signaling by plant chitin receptors (2014)
  • Author(s): Naoto Shibuya
  • Organizer: Networks in Immunity Workshop
  • Place of Presentation: 米国、デービス市
  • Year and Date: 2014-04-01 – 2014-04-03
  • Invited
• [Book] The Lotus japonicus genome (2014)
  • Author(s): T. Nakagawa, S. Okazaki, N. Shibuya
  • Total Pages: 267
  • Publisher: Springer