Interactomics of plant-pathogen interaction

• Project/Area Number: 26650106
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Nakagami Hirofumi 国立研究開発法人理化学研究所, 環境資源科学研究センター, ユニットリーダー (20435663)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: プロテオミクス / エフェクター

Outline of Final Research Achievements

Identification of pathogen effectors and their targets in host plants are important subjects for understanding plant immunity to improve disease resistance. We have optimized and established the proteomics method to identify effectors and their targets all at once.

Research Products

• [Journal Article] TDM1 regulation determines the number of meiotic divisions (2016)
  • Author(s): Cifuentes M, Jolivet S, Cromer L, Harashima H, Bulankova P, Renne C, Crismani W, Nomura, Nakagami H, Sugimoto K, Schnittger A, Riha K, Mercier R
  • Journal Title: PLOS Genetics Volume: 12(2) Issue: 2 Pages: e1005856-e1005856
  • DOI: 10.1371/journal.pgen.1005856
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Quantitative circadian phosphoproteomic analysis of Arabidopsis reveals extensive clock control of key components in physiological, metabolic and signaling pathways (2015)
  • Author(s): Choudhary M, Nomura Y, Wang L, Nakagami H, Somers D
  • Journal Title: Molecular & Cellular Proteomics Volume: 14(8) Issue: 8 Pages: 2243-2260
  • DOI: 10.1074/mcp.m114.047183
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Two Distinct Families of Protein Kinases Are Required for Plant Growth under High External Mg2+ Concentrations in Arabidopsis (2015)
  • Author(s): Junro Mogami, Yasunari Fujita, Takuya Yoshida, Yoshifumi Tsukiori, Hirofumi Nakagami, Yuko Nomura, Toru Fujiwara, Sho Nishida, Shuichi Yanagisawa, Tetsuya Ishida, Fuminori Takahashi, Kyoko Morimoto, Satoshi Kidokoro, Junya Mizoi, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki
  • Journal Title: Plant Physiology Volume: 167 Issue: 3 Pages: 1039-1057
  • DOI: 10.1104/pp.114.249870
  • Peer Reviewed / Open Access
• [Journal Article] Plasma membrane localization is essential for OsPti1a-mediated negative regulation of immune signaling in rice (2014)
  • Author(s): Matsui H, Fujiwara M, Hamada S, Shimamoto K, Nomura Y, Nakagami H, Takahashi A, Hirochika H
  • Journal Title: Plant Physiology Volume: 166(1) Issue: 1 Pages: 327-336
  • DOI: 10.1104/pp.114.243873
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Analysis of differential expression patterns of mRNA and protein during cold- and de-acclimation in Arabidopsis. (2014)
  • Author(s): Nakaminami K, Matsui A, Nakagami H, Minami A, Nomura Y, Tanaka M, Morosawa T, Ishida J, Takahashi S, Uemura M, Shirasu K, Seki M.
  • Journal Title: Molecular and Cellular Proteomics Volume: 13 Issue: 12 Pages: 3602-3611
  • DOI: 10.1074/mcp.m114.039081
  • Peer Reviewed / Open Access
• [Presentation] オオムギうどんこ病菌の付着器分泌型エフェクター候補の解析 (2016)
  • Author(s): 香口智宏、和原未季、武井博、菅井維之、野村有子、小林括平、西口正通、山岡直人、中神弘史、八丈野孝
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター・岡山・岡山
  • Year and Date: 2016-03-23
• [Presentation] MAMP応答性リン酸化タンパク質MARK1は病原菌感染による細胞死を制御する (2016)
  • Author(s): 松井英譲、野村有子、玄康洙、江草真由美、上中弘典、中神弘史
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター・岡山・岡山
  • Year and Date: 2016-03-23
• [Presentation] MAMP応答性プロテインキナーゼMRPK1のリン酸化部位変異体の解析 (2016)
  • Author(s): 玄康洙、松井英譲、野村有子、中神弘史
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター・岡山・岡山
  • Year and Date: 2016-03-22
• [Presentation] Phospho-regulation of MARK1 and MARK32 (2016)
  • Author(s): Matsui H, Hyon G, Nomura Y, Nakagami H
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学 上田キャンパス・盛岡・岩手
  • Year and Date: 2016-03-19
• [Presentation] When proteomics meets Marchantia - Highroad to understand plant immune signaling - (2016)
  • Author(s): Nakagami H
  • Organizer: Special Symposium for International Marchantia Training Course
  • Place of Presentation: 京都大学・京都・京都
  • Year and Date: 2016-03-03
  • Int'l Joint Research / Invited
• [Presentation] Towards durable and broad-spectrum resistance: Post-genomic strategies to understand conserved immune system in land plants (2015)
  • Author(s): Matsui H, Yotsui I, Hyon G, Nomura Y, Nakagami H
  • Organizer: Tsukuba Global Science Week
  • Place of Presentation: つくば国際会議場・つくば・茨城
  • Year and Date: 2015-09-28
  • Int'l Joint Research / Invited
• [Presentation] 薬剤師が植物免疫システムを考える (2015)
  • Author(s): 中神弘史
  • Organizer: 日本植物病理学会 “「若手の会」の統合を目指して”
  • Place of Presentation: 明治大学駿河台キャンパス
  • Year and Date: 2015-03-30
  • Invited
• [Presentation] エンバクの細胞表面に存在するチオレ ドキシンは宿主特異的毒素ビクトリン の標的である (2015)
  • Author(s): 兼市大輝、桑田啓子、斎藤隆一 郎、中神弘史、中屋敷均、土佐幸雄、眞山滋 志、秋光和也、多田安臣
  • Organizer: 平成27年度日本植物病理学会大会
  • Place of Presentation: 明治大学駿河台キャンパス
  • Year and Date: 2015-03-30
• [Presentation] Proteomics to understand pathogen-recognition systems at plant cell surface and their downstream events (2015)
  • Author(s): Hidenori Matsui, Izumi Yotsui, Gang-Su Hyon, Yuko Nomura, Hirofumi Nakagami
  • Organizer: Front lines of plant cell wall research
  • Place of Presentation: Kinsho Hall in Todaiji Temple Culture Center, Nara
  • Year and Date: 2015-03-20
  • Invited
• [Presentation] Dead or Alive: early MAMP-responsive phosphoprotein watches hypersensitive cell death induction (2015)
  • Author(s): Hidenori Matsui, Yuko Nomura, Hirofumi Nakagami
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学世田谷キャンパス
  • Year and Date: 2015-03-16
  • Invited
• [Presentation] エンバクの細胞表面に存在するチオレドキシンはエフェクタービクトリンの標的である (2015)
  • Author(s): 兼市大輝、中神弘史、斎藤隆一郎、中屋敷均、土佐幸雄、眞山滋志、多田安臣
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学世田谷キャンパス
  • Year and Date: 2015-03-16
• [Presentation] MAMP-recognition system in Marchantia polymorpha (2014)
  • Author(s): Izumi Yotsui, Hidenori Matsui, Yuko Nomura, Ryuichi Nishihama, Takayuki Kohchi, Hirofumi Nakagami
  • Organizer: Marchantia Workshop 2014
  • Place of Presentation: 百年記念館、神戸大学
  • Year and Date: 2014-12-09
  • Invited
• [Presentation] MAMP-responsive phosphoprotein MARK1 negatively regulates cell death during pathogen infection (2014)
  • Author(s): Hirofumi Nakagami
  • Organizer: 第12回日仏植物科学ワークショップ
  • Place of Presentation: 弥生講堂、東京大学
  • Year and Date: 2014-10-28
  • Invited
• [Presentation] オオムギうどんこ病菌の付着器から分泌されるエフェクタータンパク質の同定 (2014)
  • Author(s): 八丈野孝、菅井維之、野村有子、佐藤繭子、若崎眞由美、香口智宏、小林括 平、西口正通、豊岡公徳、中神弘史、山岡直人
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 富山
  • Year and Date: 2014-09-27 – 2014-09-28
• [Presentation] Phosphoproteomics as a tool to unravel plant signal transduction pathways (2014)
  • Author(s): Hirofumi Nakagami
  • Organizer: 日本植物学会 第78回大会
  • Place of Presentation: 明治大学生田キャンパス
  • Year and Date: 2014-09-12
  • Invited
• [Presentation] 植物のプロテオミクス (2014)
  • Author(s): 中神弘史
  • Organizer: 日本プロテオーム学会2014年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2014-07-17
  • Invited
• [Presentation] Phosphorylation in plant immunity (2014)
  • Author(s): Hirofumi Nakagami
  • Organizer: 第14回日本蛋白質科学会年会
  • Place of Presentation: ワークピア横浜
  • Year and Date: 2014-06-26
  • Invited
• [Presentation] MAMP-responsive phosphoprotein MARK1 negatively regulates cell death during pathogen infection (2014)
  • Author(s): Hirofumi Nakagami, Hidenori Matsui, Yuko Nomura, Ken Shirasu
  • Organizer: 13th international symposium of plant protein phosphorylation
  • Place of Presentation: University of Missouri, Columbia, Mo. USA
  • Year and Date: 2014-05-29
  • Invited