| Project/Area Number |
26650107
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | Ehime University (2017)
Hokkaido University (2014-2016) |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 集光特性 / 固定脳 / 生体脳 / 超解像顕微鏡 / 生体内集光特性 / 透徹化 / 収差 / ベクトルビーム / in vivoイメージング / 固定脳イメージング / 自己治癒効果 |
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| Outline of Final Research Achievements |
Evaluation of the effect of vector beam in fixed brain slice implanted fluorescent beads suggests that it shows different optical characteristics and focusing properties even in linearly polarized light. Next, Evaluating the optical properties in the fixed brain with a novel clearing reagent, conditions of that were maintained at a depth of several micrometer from the surface. Therefore, we tried to be applied the methods to super-resolution microscope (structured illumination microscope), the effect was obtained in the deep region and the spine structure of neuronal synapse in more detail which was not normally observable. Next, we tried to analyze the optical properties in the living brain, and found some conditions by adjusting the angle of the cover glass and the refractive index of the immersion liquid.
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